lab associate

Yu-Fan Tu

***** ******* **** *** *, Fremont, California, 408-***-****, *******@*****.***

OBJECTIVE

I am seeking any position allowing me to use my qualification, such as lab associate.

EDUCATION

Biochemistry, Bachelor of Science, University of Oregon, Department of Chemistry,Eugene, OR

LABORATORY EXPERIENCE

July 2010 to September 2010 – Laboratory Assistant at National Taiwan University Institute of Biochemical Science Taipei, Taiwan.

Laboratory procedures utilized on a regular basis

Purification of plasmid DNA

Restriction enzyme digestion and separation, by agarose gel electrophoresis, of plasmid DNA.

Gel isolation of digested plasmid vector and PCR fragment ligation of PCR product.

Inoculating small-scale cultures with E.coli transformant

Inoculation and IPTG induction of large-scale culture

Ammonium sulfate precipitation of cell lysate

Protein purification using DEAE Ion-exchange chromatography

LABORATORY SKILLS

1. Qualitative analysis and structure determination of unknown organic compounds.

2. DNA manipulation

3. Cloning of gene using plasmid in E.coli.

4. Protein purification (Akaline phosphatase)

5. DEAE Ion-Exchange chromatography

6. SDS-Page analysis

7. Immunoblot analysis

8. Two- component colorimetry

9. Determination of concentration by atomic absorption spectrometry

10. Determination of concentration ratio of a mixture of three known organic compounds by

internal standard method and NMR analysis.

11. Determination of ion concentration by Potentiometry

12. Quantitative HP liquid chromatography analysis.

13. Identify the component of a mixture of volatile organic compounds, by using gas

chromatography-mass spectrometry

14. Determination of ion concentration by ion chromatography

ADDITIONAL WORKING EXPERIENCE

July 2004 to September 2004 - 7-11 Convenience Store Clerk, Taipei, Taiwan

• Responsible for managing customer experience, inventory and product display

July 2007 to September 2007 - Office Assistant at Hedgehogging Fortune Investment, Taipei, Taiwan.

• Responsible for collecting, classifying and recording all statistics such as cost, benefit, value of stock increased, value of stock decreased, transaction amount and handling all money transfers with the local bank.

September 2010 to June 2011 - Chinese Student Association Volunteer, University of Oregon, Eugene, Oregon

• Member of Freshman Orientation Team

• Hosting social events to build a community among the freshman

• Hosting China Night to introduce Chinese tradition to the UO campus

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