Project Plant

Hardik Patel

**** *** ******* *****, ******, TX - 75063; 806-***-****, ****.****@*****.***

Objective

Seeking an opportunity to work as a key player in challenging & creative environment where I can utilize and enhance my skills and knowledge of Biotechnology.

Laboratory Skills

Molecular Biology & Biochemical Techniques

Genomic DNA, RNA extraction & purification, Cloning, Sub-cloning, Nucleic Acid quantification, PCR, RT-PCR, Designing Primer, SDS-PAGE, Gel electrophoresis, Transformation, MiniPrep, in-gel purification of DNA, Restriction digestion, Colony PCR, site-directed mutagenesis, Protein purification and isolation, Bradford assay, Western Blotting, ELISA.

Plant tissue Culture Techniques

Preparation of media, Sterilization techniques, Inoculation and sub-culture of callus.

Software tools

Bioedit, Vector NTI, Lasergene, Rasmol, BLAST.

Education

Master of Science in Biotechnology, Texas Tech University, Lubbock, Texas.

Graduated - May 2011

GPA-3.78

Bachelor of Technology in Biotechnology, Padmashree Dr. D Y Patil University, Mumbai, India. Graduated - May 2009

Relevant Courses

Introduction to Biotechnology- Genetics, Proteomics

Methods in Biotechnology

Statistical Concepts for Business and Management

Advanced Plant Biotechnology

Biomedical Informatics

Real-Time PCR

Marketing Concepts and Strategies for Business Management

Responsible Conduct of Research & Bioethics

Scientific Communication Skills

Food and Agricultural Biotechnology

Molecular Biology

Cell Biology

Work Experience and Projects

Texas Agrilife Research Station/ Texas Tech University - Research Assistant - July 2010-Feb 2012

Projects handled-

• Screening of the mini-core collection of Arachis hypogea (peanuts) for Acquired thermotolerance. (Project work carried out in U.S. Department of Agriculture, Lubbock, Texas)

• Parental screening of peanuts for DNA level polymorphism.

- DNA extraction, PCR using different primers, Gel electrophoresis, Fragment Analysis of amplified products with CEQ- 8000, data entry.

• Developing genetic linkage map of BC1 tetraploid peanuts.

• DNA extraction and amplification of algae samples by use of PCR.

Other Lab Responsibilities -

- Maintaining Lab records and notebook, Maintenance of Chemicals, Maintaining log of equipments, Making Stock Solutions, Watering greenhouse.

- Working on publishing a scientific paper on Arachis hypogea project in a scientific journal as the first author.

Texas Tech University, Lubbock, Texas - Academic Project Aug 2009-Dec 2009

• Cloning, Over-Expression, Purification, Biochemical Characterization and Functional studies of recombinant Carbonyl reductase.

- Bacterial gene cloning in vectors and its successful transformation in bacteria using competent E Coli JM109

- Performed isolation, purification, characterization of proteins.

Dr. Bhiwgade’s Biotech Research Centre, Mumbai - Research Assistant - Dec 2008-May 2009

• Molecular Phylogenetic Analysis of Urginea species.

- Isolation of plant samples, DNA extraction, primer design, PCR with different combinations of primers, gel electrophoresis, PCR clean-up, DNA sequencing.

- Determine homology in samples that showed PCR amplification using BLAST tool.

Achievements

Received the competitive Scholarship during the two semester of ‘Masters in Biotechnology’ at Texas Tech University.

Received the competitive Scholarship from the Department of Plant & Soil Science for fourth Semester of ‘Masters in Biotechnology’ at Texas Tech University.

Extracurricular Activities

Actively participated in the events conducted by India Students Association at Texas Tech University for the years 2009-10 and 2010-11.

Served as a volunteer to help organize ISA Cultural event 2010-11.

Represented Dr. DY Patil University in Cricket and Soccer Tournaments.

Volunteered in Fine Arts Committee for Species 2008 Annual Cultural University event.

Successfully cleared Maharashtra Cadet Corps Examination.

References available upon request.

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