D. Scott Snyder

** ******** **

Greenwood Lake, NY 10925

845-***-****

n123ps@r.postjobfree.com

Education:

B. S., Chemistry, University of Idaho (1987)

Graduated with a 3.8 GPA.

M. S., Biochemistry, Medical College of Wisconsin (1990)

Thesis: Interaction of Elongation Factor 3 from the Yeast S. cerevesiae with Azido ATP:

Study of the enzyme kinetics of a protein involved in yeast translation

Ph. D., Cell Biology and Biological Chemistry, Duke University, (2000)

Mentor: Thomas J. McIntosh

Dissertation: Structure and Antibiotic Permeability of Bacterial Lipopolysaccharide Bilayers

Experience

Senior Research Scientist, Analytical Research and Development, Pfizer Inc.

Research Projects: Conclusively demonstrated a lack of low molecular weight aldehydes in the carbohydrate based vaccine Prevnar 13, allowing FDA approval of a multibillion dollar product. Used HPLC, LC-MS and GC-MS to fully characterize different forms of rLP2086, a vaccine candidate for meningitis, also allowing company to meet FDA commitments. Demonstrated phase 2 and phase 3 production materials of rLP2086 were consistent. Consultation on difficult problems in carbohydrate chemistry. Modified GC/MS fatty acid analysis protocol to detect previously undetectable fatty acids, improve accuracy 10 fold, improve precision over 4 fold all with a decreased chromatographic run time. Developing carbohydrate analysis protocol which should be superior to the state of the art.

Managerial Duties: Interviewing, training and supervising laboratory personnel. Laboratory safety manager.

General Duties: Responsible for promoting Pfizer corporate image through Pfizer social networking site, Think Science Now.

Post Doctoral Research Associate, Complex Carbohydrate Research Center, Athens Georgia (2004 - 2009)

Teaching Responsibilities: NIH Course in Carbohydrate Analysis, DOE Course in Glycoprotein Analysis, Course in LPS Extraction Techniques.

Research Projects: Successfully characterized of a wide variety of compounds including polysaccharides, fatty acids, lipopolysaccharides and glycoproteins using HPLC, GC, GC-MS, LC-MS, MALDI, TLC, UV Visible spectroscopy and SEC. Work was performed in a GLP service lab.

Lab Manager, Integrated Microscopy Core, Baylor College of Medicine, Houston, TX(2003-2004)

Teaching Responsibilities: Annual hands on microscopy course – Both lectures and labs. Training students, technicians, post doctoral fellows and faculty in microscopy. Consultations on special projects.

Managerial Duties: Responsible for developing new techniques in microscopy such as FRET, FLIP and spectral imaging applications, training users, maintaining equipment, grant writing to obtain new equipment such as high throughput microscopes and general managerial duties in regional microscopy facility.

Research Projects: Application of above techniques to study of estrogen receptor interaction with DNA. Application of physicochemical rate laws to nuclear receptor interactions.

Researcher and Confocal Systems Manager, National Institutes of Health, Rocky Mountain Laboratories, Hamilton MT (2001-2003)

Teaching Responsibilities: Training new users in confocal microscopy. Involved in BRASS (Biomedical Research After School Scholars program) which introduced middle school students to biomedical research.

Managerial Duties: Managing confocal facility, repair and upkeep of equipment.

Research Project: Responsible for independent research examining the inflammatory response of cultured mammalian cells in response to the TLR2 lipoprotein ligand OspA and the internalization of OspA in such cells using NF kappa B localization assays, as well as TNF and IL-1 ELISAs and extensive live cell confocal microscopy.

Post Doctoral Fellow, National Institutes of Health, Rocky Mountain Laboratories, Hamilton MT (2000-2001)

Research Project: Determination of the mechanism of action of mycolactone, a toxin derived from Mycobacterium ulcerans.

Research Analyst for Ribi Immunochem Inc., Hamilton, MT (1990-1995)

Process development and scale up of the now highly successful vaccine adjuvant 3D-MPL using HPLC, LC, GC, TLC, NMR, UV/VIS and wet chemical methods

Refined lab bench procedures for industrial setting (scale-up) and for quality control

Developed novel analytical methods for GMP adjuvants and vaccines assayed effects of inflammatory mediators and derivatives on cell cultures and in animal models using both the ELISA and NO assays

Interviewed and trained research technicians

Conducted facility tours for investors, lawmakers and the public

Received 3 raises and 1 promotion in this position

Publications

SR Hagen, JD Thompson, DS Snyder and KR Myers, Analysis of a Monophosphoryl Lipid A Immunostimulant Preparation from Salmonella R595 by HPLC, Journal of Chromatography, 767 (1997) pages 53-61

DS Snyder, D Kim and TJ. McIntosh, LPS Bilayer Structure: Effect of Chemotype, Core Mutations, Divalent Cations and Temperature, Biochemistry, v38, number 33 (1999) pages 107**-*****

K Kulkarni, DS Snyder, and TJ McIntosh, Adhesion Between Cerebroside Bilayers, Biochemistry, v38, number 46, (1999) pages 152**-*****

DS Snyder and TJ. McIntosh, The LPS Permeability Barrier: Correlation of Antibiotic Permeabilities with Antibiotic Susceptibilities and Fluorescent Probe Binding Kinetics Biochemistry, v39, number 38 (2000) pages 11777-87

EB Guererro, TJ McIntosh, GR Barclay, DS Snyder, RJ Gibbs, MG Mythen and IR Poxton, Preparation and Preclinical Evaluation of a Novel Liposomal Complete Core Lipopolysaccharide Vaccine, Infection and Immunity, v68 number 11 (2000) pages 6202-8

DS Snyder and PLC Small, Staining of Cellular Mitochondria with LDS-751, JIM, v257 (2001) pages 35-40

DS Snyder and PLC Small, Uptake and cellular actions of mycolactone, a virulence determinant for Mycobacterium ulcerans. Microb Pathog. 2003 Feb;34(2):91-101.

D Welty, DS Snyder. Internalization of OspA in rsCD14 complex and aggregated forms.

Mol Microbiol. 2003 Nov;50(3):835-43.

KA Jackson, DS Snyder, MA Goodell, Stem Cell Engraftment: Distinguishing GFP from Autofluorescence. Stem Cells. 2004;22(2):180-7

DS Snyder, DL Gibson, C Heiss, WW Kay, P Azadi Structure of a capsular polysaccharide isolated from Salmonella enteritidis. Carbohydr Res. 2006 Oct 16;341(14):2388-97.

J Li, F Ahmed, M Mandal, P Azadi, DS Snyder, C Ryder, T Zahrt, R Gogal, TJ Inzana, Identification of Francisella tularensis LVS specific genes by suppression subtractive hybridization and characterization of an O-antigen deficient mutant Microbiology 153 (2007), 3141-3153

P Zhang, DS Snyder, P Feng, P Azadi, S Zhang, S Bulgheresi, K Sanderson, J He, J Klena, T Chen, Role of N-Acetylglucosamine within core lipopolysaccharide of several species of gram negative bacteria in targeting DC-SIGN (CD209) Journal of Immunology, 2006, 177: 4002-4011

PJ Brett, MN Burtnick, DS Snyder, JG Shannon, P Azadi, FC Gherardini Burkholderia mallei expresses a unique LPS mixture that is a potent activator of human antigen presenting cells Mol Microbiol (2007) 63 (2), 379–390

DS Snyder, B Brahamsha, P Azadi, B Palenik Structure of a Compositionally Simple Lipopolysaccharide from Marine Synechococcus J. Bacteriology (2009) 191 (17), 5499-5509

Aloka B. Bandara, Anna E. Champion, Xiaoshan Wang, Gretchen Berg, Michael A. Apicella, Molly McLendon, Parastoo Azadi, D. Scott Snyder, Thomas J. Inzana Isolation and Mutagenesis of a Capsule-Like Complex (CLC) from Francisella tularensis, and Contribution of the CLC to F. tularensis Virulence in Mice PloS One 6(4), 1-14

Patents

Vaccine Against Lipopolysaccharide Core, Elliott Bennett Guererro, George Robin Barclay, Ian Raymond Poxton, Thomas James McIntosh and David Scott Snyder United States Patent Number 6,749,831 Issued June 15, 2004

Method for the Production of 3 O Deacylated 4’ Monophosphoryl Lipid A, Kent Myers and Scott Snyder United States Patent Application Number 200********, Filed February 13, 2003 (pending)

Invited Presentations

A Tale of Two Ligands University of North Texas Health Sciences Center at Fort Worth, April 2002

Evaluating Receptor Interactions with Molecular Scaffolds Center for Subsurface Sensing and Imaging Systems, Northeastern University, May 2004

Evaluating Receptor Interactions with Molecular Scaffolds National Jewish Medical and Research Ctr. May, 2004

Abstracts

Gibson, D.L., White, A.P., Snyder, D.S., Azadi, P., and W. W. Kay. A

novel capsular exopolysaccharide is co-regulated with the Salmonella

extracellular matrix. 2005 Gordon Research Conference: Microbial Adhesion, Salve

Regina University, RI and the International Union of Microbiological

Societies, San Francisco, California.

Gibson, D.L., White, A.P., Snyder, D.S., Martin, S.G., Azadi, P., and W. W. Kay Identification and Characterization of Novel Extracellular Polysaccharides Associated with the Extracellular Matrix of Salmonella enterica Enteritidis. 2005 Canadian Bacterial Disease Network Meeting, Banff, A.B.

Deanna L. Gibson, Aaron P. White, D. Scott Snyder, Sandra G. Martin, Parastoo Azadi, and William W. Kay Identification and characterization of novel extracellular polysaccharides associated with the extracellular matrix of Salmonella enterica Enteritidis, 2005 American Society of Microbiology Meeting: Salmonella, Sardinia, Italy

Deanna L. Gibson, Aaron P. White, D. Scott Snyder, Sandra G. Martin, Parastoo Azadi, and William W. Kay Identification and characterization of novel extracellular polysaccharides associated with the extracellular matrix of Salmonella enterica Enteritidis, 2005 American Society of Microbiology Meeting: Biofilms, Victoria, B.C.

DS Snyder, KL Whitaker and KR Myers, Structural and Biological Characterization of Unmodified Re Lipopolysaccharide by Normal Phase Chromatography 1995 FASEB meeting

DS Snyder and TJ McIntosh, Bacterial Antibiotic Susceptibility and Lipopolysaccharide Structure/Permeability 1999 International Endotoxin Society Meeting

Scholarships, Honors and Awards

Richard Asofsky Award for Special Achievement in Equal Employment Opportunity, Hoggsey-Cossey Award, WMA Scholarship, 5th Marine Division Scholarship, Wyeth Vaccines Globe Award

NIH R01GM58432-01 Structure/Permeability of Lipopolysaccharide Membranes written with Thomas J. McIntosh

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