Research Associate

SMIT PATEL

** **. ********* ****** #****, Boston, MA 02120

240-***-**** • ************@*****.***

RESEARCH ASSOCIATE

Highly organized, detail-oriented research professional with in-depth knowledge and experience across multiple disciplines of biotechnology, molecular biology, and analytical chemistry. History of contributing to new protocol testing and establishment. Proven success in completing projects on time or ahead of schedule in fast-paced, highly deadline-driven environments. Demonstrated ability to conduct independent research and collaborate effectively with other team members.

AREAS OF EXPERTISE:

• Molecular Cell Biology & Immunoassays

• Biochemical Assays

• Protocol Testing & Development

• Experiment Design & Delivery • Pharmaceutical Product Development

• Quality Control & Quality Assurance

• Statistical Data Analysis

• Troubleshooting & Problem Resolution

TECHNICAL PROFICIENCIES

Molecular Biology Techniques: High Throughput Screening (HTS) of Biologically Active Compounds, PCR, RT-PCR, Cell Culturing, Cell Passaging, Cell Lysis, DNA/mRNA Extraction, DNA Purification, cDNA Synthesis, Restriction Digestion, Ligation, Gel Extraction and Gel Analysis, Enzyme Kinetic Study, Immunohistochemistry, BCA Assay, Bradford Assay, Nanoparticle Formulation and Evaluation

Separation & Detection Techniques: Protein Extraction, Separation, and Purification of Protein of Interest by HPLC, Affinity Chromatography, RPLC, SDS-PAGE, NativePAGE, Detection Techniques including ELISA (Direct, Indirect and Sandwich), Western Blot, LC-MS Analysis of Biomolecules, Peptide Mapping, and Bottom-up LC-MC Analysis

Equipment: Platemate 2X2 Liquid Handler, BioTek Synergy HT Plate Reader, Sciclone ALH 3000 Work Station, QIAcube Miniprep Machine, Benchpro for DNAmaxiprep, Qpix2 Colony Picker & Rearray Machine, Malvern Zeta-Sizer

PROFESSIONAL EXPERIENCE

EMD SERONO, INC., Billerica, MA

Research Assistant, 7/2011 – 12/2011

Gained hands-on experience in core molecular cell biology and automation techniques in fast-paced, team-oriented and independent work environment. Coordinated High Throughput Screening (HTS) of active compounds. Performed high throughput IgG cloning, PCR, restriction digestion, ligation, transformation, colony picking, PCR amplicon purification, DNA purification by DNAminiprep and DNAmaxiprep, gel analysis, and maintenance of glycerol stocks of potential clones. Operated Platemate 2X2 liquid handler, Sciclone ALH 3000 work station for DNA purification, QIAcube miniprep machine, Benchpro for DNAmaxiprep, Qpix2 colony picker, and rearray machine. Evaluated, analyzed, and presented statistical data on a weekly basis. Accurately maintained electronic records of daily experiments.

Effectively completed all projects and tasks in a highly deadline-driven environment.

Decreased contamination by testing and establishing protocols for Qpix2 colony picking machine for picking potential clones from 48-well agar plate

Successfully developed new protocol for rearraying of picked clones.

CENTER FOR DRUG DISCOVERY, NORTHEASTERN UNIVERSITY, Boston, MA

Research Assistant, 5/2011 – 7/2011

Performed independent work in highly innovative environment. Assisted Ph.D. candidate in formulating protein nanodisc with localization of CB1 receptors for structural study. Participated in cell lysis as well as extraction, separation, and purification of membrane protein of interest by HPLC. Detection of the protein of interest by ELISA in 96-well format and Western-Blot analysis.

PHARMACEUTICAL SCIENCE LABORATORY, NORTHEASTERN UNIVERSITY, Boston, MA

Graduate Research Assistant, 1/2011 – 4/2011

Gained in-depth knowledge of various molecular cell biology techniques while working in group and presenting weekly reports to Principal Investigator in highly deadline-driven environment. Performed specific techniques of cell culturing and passaging, transfection, DNA and protein extraction, Cytotoxicity assay, BCA-assay, Immuno-histochemistry, enzyme-kinetics studies, ligand binding assay, protein purification by affinity chromatography, nanoparticle formulation, SDS-PAGE analysis, ELISA , Western blot analysis, PCR, qPCR, restriction digestion, and gel extraction.

Played vital role in project involving design of new pharmaceutical for treating acute renal failure.

BIOTECH APPLICATION LABORATORY, NORTHEASTERN UNIVERSITY, Boston, MA

Graduate Research Assistant, 8/2010 – 12/2010

Developed in-depth knowledge and hands-on experience in various chromatography, protein separation, identification, and purification techniques. Utilized RPLC, affinity chromatography, bottom-up LC-MS, Peptide mapping, SDS-PAGE, Native PAGE, ELISA, and Western blot. Conducted independent research and collaborated with peers in laboratory. Compiled, analyzed, maintained, and presented data to lab instructor on a weekly basis.

LINCOLN PHARMACEUTICAL, Ahmadabad, India

Qualitative and Quantitative Analyst, 5/2009 – 7/2009

Conducted a variety of biochemical assays of multiple drugs to measure estimated content of active pharmaceutical ingredients in fast-paced, deadline-driven environment. Coordinated Quality Control (QC) and Quality Assurance (QA) of processed pharmaceutical products in GLP/GCLP regulated environment. Measured drug properties and provided calculations and documentation of steps taken.

EDUCATION & CREDENTIALS

Master of Science in Biotechnology, Molecular Track, 6/2012

NORTHEASTERN UNIVERSITY, Boston, MA

3.66/4.0 GPA

Bachelor of Pharmacy, 5/2009

BARODA COLLEGE OF PHARMACY, GUJARAT UNIVERSITY, Gujarat, India

3.88/4.0 GPA.

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