Project Training

Resume of

Damodara Rao.G

Carrier Objective:

Have +8 years of industrial research experience with last 2.9 years as a Research Scientist in biopharma. Have experience in the fields of Molecular biology, Immunology & Protein Purifications. I have excellent analytical, organizational skills, a good observer, creative and productive, largely self-directed and able to work both independently and as a team member. Highly flexible with fast grasping and quick delivering of committed work.

Professional Experience:

From To Designation Address

2011/08 Till Date Biology Tutor Richmond, British Columbia, Canada.

From To Designation Address

2008/09 2011/06 Research Scientist Panacea Biotec Private Limited, India. www.panaceabiotec.com.

Main Duties

Primer Designing, Cloning, Expression and Molecular level Gene Characterization of Recombinant Protein of therapeutic Importance (Tumor Necrosis factor Receptor II / Etanercept). Involved in the development of Single Vector and Double Vector strategies.

Molecular level Characterization of Recombinant Blood clotting Factor VIII & Von Willebrand factor (vWF).

Identification of Expression Enhancers & Construction of Recombinant Expression vector with the addition of Enhancer Elements for the Increase of protein yield of the therapeutic molecule.

Optimization of ELISA for the quantification of Bio molecules using different ELISA Readers. Performed Direct, Indirect & Sandwich ELISA for the quantification of Bio-similar’s & Therapeutic proteins of High Importance.

Expertise in Mammalian Cell Culture involves in Maintenance, Transfection (Lipofectamine, Dreamfect & Freestyle max reagents) Selection of Clones by drug, Kill curve Generation of drug towards CHO Cell lines, Optimization of Antibiotic Concentration /Amplification of Gene, Single Cell Dilution Cloning, optimization of serum free adaptation process using different media &performed Serum free adaptation of cells/yield Checking and finally done Scale up from Flask to lab scale Fermenter Level of Bio-similar or follow on biologics.

Small-scale Therapeutic protein production from Flask to spinner level, Involved in the Molecular level characterization.

Frequently use to Interact with the IPR department and involved in the development of Expression Enhancers which had helped for the better increase of the Molecule’s yield.

Responsible for meeting regulatory requirements associated with working Molecule product validation.

Responsible for executing scientific research and development strategies for the company.

Investigating concepts, planning laboratory research, spokesperson on corporate research, interacting with various departments and supporting all regulations.

From To Occupation Address

2006/06 2008/09 Senior Research Fellow II Avesthagen Technologies, India.

www.avesthagen.com

Main Duties

Generation of ScFv Phage Display of Proteins of Therapeutic Importance.

Involved as a lab member in the Establishment of molecular biology lab which includes right from the start ordering of chemicals, Discussions with the vendors, performing lab work & regular maintenance of the lab Instruments.

Involved in Cloning, Partial Purification & Lyophilization of Recombinant molecules of therapeutic Importance (Tumor Necrosis factor Receptor II).

Optimization of ELISA & Performed Direct, Indirect and Sandwich ELISA for Quantification of Bio-similar Molecules.

Involved In the purification of Tumor Necrosis factor Receptor II that got expressed & interacted with the team for the process development of the molecule for better increase of yield.

Worked with various filtration systems (TFF systems of Millipore & Pall Life Sciences) for the purification of the Molecule.

Involved in the lyophilization cycle optimization (finding out the Eutectic point) and Involved in the Formulation and Solid Lyophilization of Tumor Necrosis factor Receptor II. Lyophilization is done using Lyolab Lyophilized.

Attended Various Seminars Inside the Company & other places on various Molecular biology & Protein Purification process areas.

Maintenance of Experimental observations, compile and use to summarize complex and large data sets and use to prepare reports, and presentations at group & project Meetings.

Done Standardization and Maintenance of Standard Operating Procedures (SOP) of Equipments, Experiments, Regular Work Data Sheets and Presentations.

From To Occupation Address

2002/09 2006/05 Project Trainee & Research Associate Magene Life Sciences Private Limited.

www.magenelifesciences.com

Main Duties Preparation of Buffers, Cell culture Media, plasmid Isolations (Mini, Midi & Maxi kits).

Assisted the Senior Scientists in the Cloning and protein purifications and routinely use to perform (Agarose gel, SDS PAGE, DNA & Protein Quantification and checking the purity by Nanodrop and U.V Spectrophotometer).

Got trained on various Molecular biology works on Bacterial & Mammalian cells.

Involved in the Cloning of Biomolecule of Therapeutic Importance (Dipeptidyl peptidase DPP-9 & DPP-8).

Got trained on the Chromatographic systems & worked on the chromatographic Systems (Akta Prime & Akta Purifier – Amersham).

Trained on various Chromatographic Apparatus (Akta Purifier & Akta Explorer) and Involved in the purification process as per the GLP & GMP Guide lines.

Collection of Literature on Molecular Biology aspects of the Molecule & use to take into consideration for the development of the project.

Attended Various Seminars Inside the Company & other places on various Purification process.

Trained on preparation of SOP’s for Experiments & for Apparatus for the Molecular Biology lab.

From To Training Address

2002/05 2002/07 Project Training -2 Post Graduate Institute of Medical Education & Research. (PGIMER), Chandigarh, India http://pgimer.nic.in/

Project Invitro Mutagenesis of Entero Agrregative E.Coli.

Trained on the working Priniple of FACS Instrument & quantified the lymphocytes of normal Healthy Humans & Cancer Patients.

Trained on FACS & Under the Scientist Guidance quantified Normal Healthy Cells & Apoptotic Cell by FACS (Becton & Dickenson).

From To Training Address

2001/03 2001/06 Project Training -1 Agharkar Research Institute, Agharkar Road, Pune, India http://www.aripune.org/

Project Cloning, Expression & purification of Alkaline Proteases from Bacillus Sphericus.

Studied the effect of Triton X 100, Tween-20 & DMSO Impact on the Alkaline proteases at different time periods ( 0-72hrs) & Checked the Enzyme Activity by lowry Method & Protein Yield by Bradford Methods.

Trained on performed partial Purification of the protein by Centrifugation, Ammonium Sulphate Precipitations& Dialysis Methods.

From To Institutional Training Address

2001/03 2001/06 Institutional Training Pasteur Institute of India, Coonor, India. http://www.pasteurinstituteindia.com/

Training Observed the DTP & Rabies Vaccine manufacturing & Quality Control of the Vaccines in Large Scale.

Educational Background:

From To Qualification % Secured Name of the University

2000/06 2002/03 Master of Science in Biotechnology 70% Acharya Nagarjuna University Campus, Guntur, Andhra Pradesh, India, Asia.

1997/06 2000/04 Bachelor of Science in Chemistry 70% P.B. Siddhartha College of Arts & Science. Vijayawada, Andhra Pradesh, India, Asia.

From To

2002/03 Till Date Overall Technical Skills & Expertise

Overall Technical Skills & Expertise Direct, Indirect and Sandwich ELISA for Quantification of Various Molecules & Bio-similar Molecules.

Cloning of PCR product into Clonejet PCR Cloning Kit (Fermentas) & Cloning of genes into Expression Vectors.

Bioassay of Blood Clotting Factor by using Kabi Coatest Kit.

Transfection of target DNA Inserted into Mammalian Expression Vector by Lipofectamine 2000 (Invitrogen) & Dreamfect Methods.

Isolation of lymphocytes from Human blood by using Ficoll –Paque Plus.

Isolation of mRNA by Polytract kit (Promega).

RNA isolation by Trizol, RNAgent methods and RNA estimation by Ribogreen Method.

cDNA Synthesis by first strand cDNA synthesis kits (Invitrogen & Promega Kits).

Extraction of DNA from Agarose Gel by using Q1 AQuick Kit method.

Isolation of plasmid DNA by using standard kits like Promega, Eppendorf & iNTRON kits.

Genomic DNA Isolation from Mammalian cells by standard Methods

Separation of Receptor bound ligand from unbound ligand by Pall Vacuum Chamber.

Clarification of the proteins by using Millipore Millistak filters membranes.

Estimation of proteins & RNA by Bradford, BCA, Biuret, Ribogreen & Folin’s method.

Detection of Protein’s by Native-PAGE, SDS-PAGE & Western blotting Techniques.

Purification of recombinant proteins by using AKTA Purifier & AKTA Explorer systems.

Quantification of RNA, DNA & Proteins by using Nanodrop system

Observation on large-scale production & Purifiaction of DTP &Rabies Vaccines.

Lyophilization of recombinant expressed protein by using Lyolab Lyophilizer system.

Bioinformatics Software's & Computer skills:

Similarity search and sequence alignment: Clone Manager, NCBI BLAST, EBI- Fasta, EBI-Clustal W. Excellent computing skills and proficient in the use of Word, Excel, PowerPoint, Adobe Photoshop, Graphpad Prism.Unique ability to learn and work on various computer applications and software efficiently.

References:

Available upon Request

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