Research Analyst

Kanika Kapoor

*** *********** ******, **** ***, Glasgow, Scotland, G2 3LG, UK

Mobile: +44-793******* Email: **************@*****.**.**

CARRER PROFILE:

A holder of MRes in Microbiology with excellent analytical and interpersonal skills. Experience of working in different pharmaceutical companies. A highly motivated and organized individual used working on my own initiative and as part of a team.

EDUCATION:

Establishment Date Qualification

University of Glasgow Nov 2010 MRes Medical and Veterinary Microbiology (Merit)

Punjab Technical University July 2008 B.Tech Biotechnology (81%, 4th position in Merit)

EMPLOYMENT HISTORY:

Piramal Healthcare Limited, Grangemouth, Earls Road, FK3 8XG, Scotland, UK

Research Analyst (Quality control) June 2011 – Sep 2011 Responsibilities:

• Working in cGMP facility (FDA/MHRA audited) learnt 5S programmes in QC.

• Learning FDA/ MHRA regulatory compliance of laboratory, personnel and procedures.

• Effectively performed ELISA and cell based assays and related chemistry analysis using wet chemistry: UV Spectrophotometer, pH-meter, HPLC and Isoelectric focussing.

• Assisted in routine monitoring of environmental program in HPS manufacturing plants to ensure manufacturing processes are monitored within FDA regulatory guidelines.

• Assisted in routine microorganisms identification using API strips.

• Daily perform calibration and performance checks for laboratory equipments to ensure compliance with cGMP, GLP, GDP and SOPs.

Daiichi Sankyo Research Centre (Research Trainee) Dec 2008- June 2009

Working in the microbiology department, I characterized and determined susceptibility profile of Mycobacterium tuberculosis strains following COSHH regulations.

• Job sponsored by Ministry of Science & biotechnology, Govt. of India.

• Performed MIC and MPC concentration checks for clinical samples.

• Screened compounds for newly synthesized molecules (antimicrobial agents).

Daiichi Sankyo Research Centre (Biotech lab trainee) Jan 2008-Aug 2008

I worked in the biotechnology department with Dr. Sadhev on cloning and expression of cyp gene from cytochrome P450 family which is involved in the metabolism of xenobiotics in the body, in particular:

• Baculovirus expression system using TOPO cloning system.

• Estimated protein expression by reductase assay and Bradford assay.

• Microbiology:

Media preparation, Bacterial Culturing, Gram-staining, MIC checks and flow cytometery.

• Molecular Biology:

PCR, ELISA, Gene cloning, Recombinant DNA technology, DNA & RNA Extraction & quantification, Reverse Transcriptase PCR (RT-PCR), UV Spectrophotometry, SDS-PAGE gel Electrophoresis, Western blotting.

RESEARCH EXPERIENCE:

Masters was focused to demonstrate theoretical knowledge and practical skills in molecular

techniques to the study of microbial pathogenesis and new-disease causing strategies in humans and domesticated animals.

Master’s projects

Investigation of the role of single and double knockout mutants in two-component systems (TCS) of Streptococcus pneumoniae. (Jan 2010-Apr 2010, Prof. Tim Mitchell). The main objective behind the project was to construct single and double knockout mutants in TCS06 and TCS08 (followed COSHH regulations).

• Studied TCS03, TCS06, TCS09 and TCS08: involved in pilus regulation.

• Constructed single and double mutants by splice overlap extension (SOE) PCR.

• Evaluated and analysed phenotypic effects of single and double mutants through ECL.

Distribution and molecular evolution of virulence genes (hemolysin genes) in Vibrio parahaemolyticus. (May 2010-Sept 2010, Dr. Robert Davies). The objective behind the project was to determine the distribution and evolution of genes within bacterial population.

• Designed specific primers for the identification of virulence genes using CLC genomics.

• Developed multiplex-PCR for the identification of genes and performed comparative nucleotide sequence analysis using MEGA and CLUSTAL X programme.

• Prepared data review and results were published in the poster.

Bachelor’s Projects

• Identification, isolation & characterisation of Vesicular Arbuscular Mycorrhizae Fungi (VAM) and its utilization as a bio-fertiliser. (July 2007- Dec 2007).

• Gene cloning in Escherichia coli. (May 2006- July 2006, Dr. V. Verma, Indian Institute of Integrative Medicines, CSIR, Jammu, India).

KEY SKILLS:

• Excellent communication skills developed through experience in research environment.

• Research project required co-operation of partners in industry and academia and so further developed negotiation and persuasion.

• Helped undergraduates in their projects.

AWARDS/ACHIEVEMENTS:

• Biological Science Departmental Scholarship, University of Leeds (2009-2010).

• Biotech Consortium India Limited Fellowship to work in Daiichi Sankyo Research Centre. (Ministry of Science and Biotechnology, Dec 2008- June 2009, India).

• Punjab Technical University Scholarship, Merit position (Dec 2006).

HOBBIES AND INTERESTS:

As well as having a strong desire to improve the living standards of people across the globe by the development of new drugs and treatments I have a keen interest in dance; I won second prize in ‘inter-university’ group dance competition ‘PANACHE 2006’ college annual fest, as well taking a leading part in the Diwali (the Hindu festival of light) dance celebrations held in George Square in November 2009.

REFERENCES:

Dr. Ruth Sorbie (QC Manager, Stability & Production, Piramal Healthcare)

Prof. Tim Mitchell (Chair of Microbiology, University of Glasgow, Scotland)

Dr. Dilip Upadhyay (Director Infectious diseases, Daiichi Sankyo, Gurgaon, India)

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