CAREER SUMMARY

My experience includes over ** years as a cell, molecular biology and immunology Scientist with over 2 years of industry experience as Microbiologist/Assistant Technical manager. Presently working as Senior Research Scientist/Research Assistant Professor at Stony Brook University since 2007. I have multiple years of experience in cell based assays and cell culture, cell Biology, molecular biology and immunology and related analytical techniques including polymerase chain reaction, cell-based immunoassays, transfection, maintaining primary and immortalized cell lines, ELISAs, receptor binding assays and cell-based bioactivity assays, RNA isolation, genomic DNA isolation, polymerase chain reaction-based methods, and other standard molecular biology techniques. Experienced in data handling/graphing software (e.g. Excel, Graph pad Prism), analyzing and interpreting data, writing reports and presenting data, data validation, using biostatistics on data, ensuring the compliance, accuracy and timeliness of the laboratory. Supervising post-doctoral fellows, research support specialists, graduate and undergraduate students. As Post-Doctoral Fellow in the Department of Microbiology and Immunology at Medical University of South Carolina, Charleston worked on mucosal immunology for over five years. Worked also on a clinical trial on diabetes. As faculty in biotechnology, established a new cell culture facility and mentored students for their research projects in immunology and cell culture. Worked in food industry for two years. Started a new quality control and microbiology laboratory. Was responsible for the production, quality control, managing and training of junior scientists. I have over fifteen years (post-PhD) experience in various other immunological, microbiological, molecular biological, biochemical techniques, cell culture, cell based assays, flow cytometry, cell isolation and stimulation, in vivo and in vitro assays, growing of virus and bacteria, ELISA, PCR, working with animal models etc.

PROFESSIONAL EXPERIENCE

Senior Research Scientist/Research Assistant Professor, 07/2007 – Current

School of Dental Medicine, Department of Oral Biology & Pathology AND Department of Periodontics and Implantology, Stony Brook University, Stony Brook, NY

• Lab management and Research in cell, molecular biology and immunology: Managing all aspects of the research laboratory. Conducting research in cell, molecular biology, immunology, virology, bacteriology and inflammation including animal models as well as patients based research. Also supervising post-doctoral fellows, research support people, graduate and undergraduate students in research. Compiling and analyzing research data and writing research papers for publication in peer reviewed journals.

• Anti-inflammatory drug development: Started new drug development using polyphenols (new curcumin compounds) to be developed as anti-inflammatory drugs in a rat model of type 2 diabetes and periodontitis. Successfully submitted many research proposals as Principal investigator to NIH.

• Research in Periodontitis and atherosclerosis: Conducting research to prove periodontitis as an independent risk factor for atherosclerosis. Tracking the Early Events in Atherosclerosis using Multimodal Molecular Imaging using PET/MRI to track dendritic cells in animal models.

Postdoctoral Fellow, 01/2002 – 07/2007

Department of Microbiology & Immunology, Medical University of South Carolina, Charleston SC

• Research in mucosal Immunology and virology: Conducted research in the area of mucosal immunology & virology. Worked in a clinical trial on an African American population of coastal Carolina, where we looked at the oral health of the people with type 2 diabetes. Planned and conducted all experiments independently. Helped in grant writing, progress reports and animal protocols. Published research papers in top-tiered journals.

Research Scientist, 11/2000 – 12/2001

International Center for Genetic Engineering & Biotechnology New Delhi, India

• Research in parasite immunology: Conducted research in the area of malarial immunology involving patient samples and in animal model. Supervised PhD students, research support staff and undergraduate students. Supervised students in publishing research papers.

Lecturer, 01/1998 – 11/2000

Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, India

• Research and teaching in immunology, microbiology, cell culture: Directed an independent research laboratory. Established a new cell culture facility. Supervised post-doctoral fellows, PhD students, Master’s students, Research support staff and undergraduate students. Mentored research projects in tuberculosis immunology involving patients and animal models. Taught theory and laboratory in Immunology, Animal Tissue Culture, Immunotechnology, Microbiology, Cellular Biochemistry, Techniques in Biotechnology. Participated in biotechnology course curriculum development.

Research Fellow, Junior and Senior Research Fellow, Research Associate, 12/1991 - 12/1997

Post Graduate Institute of Medical Education & Research, Chandigarh, India

• Research in microbiology & immunology: Conducted research for my PhD degree in the area of parasite immunology involving clinical and basic research. Worked on clinical trials. Published research papers.

Asst. Technical Manager/Microbiologist, 12/1989 – 12/1991

Teg’s Masrado (P) Ltd and Snowhite Mushrooms, Chandigarh, India

• Quality control and new laboratory setup: Established a new quality control and microbiology laboratory. Managed production, quality control, supervision and training of junior scientists.

AREAS OF EXPERTISE/CORE COMPETENCIES/SKILLS

Immunological techniques,

ELISA, Western Blotting, Immunofluorescence, Flow cytometry (FACS), In vivo and in vitro assays, Cytotoxicity assays, cell culture, Isolating proteins from human and animal tissues, Preparing and analyzing tissue samples, Cell cryopreservation and recovery, Cell fractionation and protein extraction, Microbiological techniques, Aseptic techniques, Growing and titration of virus, Aerobic and anaerobic bacterial cultures, Identification of bacteria, Infection prevention, Quality control, Clinical research Molecular biology techniques, Isolation of RNA, DNA and proteins from cells, viral and bacterial cultures, Polymerase Chain Reaction (PCR), Reverse transcriptase PCR, Real time PCR, Biochemical techniques, Immunohistochemistry, Cell staining, SDS-PAGE, Parasitology techniques, Protein antigen purification, Signaling pathways, Animal model studies, Harvesting and Isolation of cells and tissues from animal models and patient samples, Analyzing, interpreting and documenting results, Microsoft Office, Adobe Acrobat, Adobe Photoshop, Biostatistics, SPSS, Graph Pad, Writing NIH grant proposals, Research papers, Progress reports, Animal protocols, Poster presentation, Good working knowledge of computer hardware and software, Problem solving, Ability to lead/manage people, Excellent communication skills (verbal/written/listening), Creativity/resourcefulness, Decision making/independent judgement, Interpersonal skills, Organizational skills, Planning, Team work and Time Management skills, Analyzing, Organizing, documenting results, Logical presentation in team/project meetings

EDUCATION

PhD Immunology

Post Graduate Institute of Medical Education & Research, Chandigarh, India

Post graduate Diploma Industrial Relations and Personnel Management

R.P. Institute of Communication and Management, Mumbai, India

M. Sc. (Honors) Microbiology

Panjab University, Chandigarh, India

B. Sc. (Honors) Microbiology (major), biochemistry, biophysics, biostatistics

Panjab University, Chandigarh, India

ADDITIONAL QUALIFICATIONS

• Course in Teaching Techniques from Medical University of South Carolina, Charleston SC (2006)

• Course in HIPAA Privacy Rule from Medical University of South Carolina, Charleston SC (2003)

• Course in Responsible Conduct in Research, Medical University of South Carolina, Charleston SC (2002)

• Course in Use of Personal Computers, Regional Computer Center, Chandigarh (1995)

• Course in “Safe handling of Radioisotopes” Post Graduate Institute of Medical Education & Research, Chandigarh, India (1994)

• Course in “Research Methodology and Biostatistics” Post Graduate Institute of Medical Education & Research, Chandigarh, India (1993)

PUBLICATIONS

• Research papers published in Peer-Reviewed Journals: 19

• Published Abstracts/Conferences attended: 27

EXTRAMURAL FUNDED RESEARCH/APPLIED FOR

• National Institute of Dental and Craniofacial Research/ National Institutes of Health, RO1 DE016652 "Oral Immunobiology of MCMV-infected Mouse Salivary Glands", Role: Co-investigator (2006 – 2011)

• National Institutes of Health RO3 entitled, “Modulation of signaling pathway profiles in inflammation and bone loss” Role: Principal Investigator (applied 2010)

• National Institute of Dental and Craniofacial Research/National Institutes of Health, RO3 entitled, “Curcumin-Mediated inhibition of osteoclastogenesis in an in vitro model, Role: Principal Investigator (applied 2009)

• National Institute of Dental and Craniofacial Research/National Institutes of Health, R21 entitled, “Curcumin-Mediated Tissue Remodeling in a Rat Model of Accelerated Periodontitis” Role: Principal Investigator (applied 2008, priority Score: 279)

SUPERVISOR/MENTOR

• School of Dental Medicine, Stony Brook University, Stony Brook, New York, USA, 07/2007 – Current

Research Assistant Professor, Undergraduate students supervised/supervising: 4

• Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, India, 01/1998 –11/2000

Lecturer, Masters in biotechnology student’s research projects mentored: 6

• In industry (Teg’s Masrado (P) Ltd and Snowhite Mushrooms, Chandigarh, India)

Assistant Technical Manager/Microbiologist, 12/1989 – 12/1991, Supervised & trained junior scientists and technical and non-technical staff

MEMBERSHIP IN PROFESSIONAL/SCIENTIFIC ORGANIZATIONS

• American Association of Immunologists

• International Association for Dental Research/American Association for Dental Research

• Indian Association of Medical Microbiologist (Life member)

• Association of Microbiologists of India (Life member)

• The Indian Society for Parasitology (Life member)

• Indian Association of Pathologists and Microbiologists

• The Association of Food Scientists and Technologists (Mysore)

• Society for Mucosal Immunology, USA

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