Name: Sergei Y. Vatolin

Date and Place of Birth: August 10th, 1970, Novosibirsk, Russia

Work address: Department of Translational Hematology and Oncology Research,

Taussig Cancer Center,

The Cleveland Clinic Foundation

9500 Euclid Ave.,

Cleveland, OH 44195

m3pr3s@r.postjobfree.com

work: 216-***-****

Home address: 564 Elmwood Rd.

Bay Village, OH 44140

Phone: 716-***-****

e-mail: m3pr3s@r.postjobfree.com

Professional Experience and Expertise

2009-Present Project Scientist, Department of Translational Hematology and Oncology Research,

Taussig Cancer Center, The Cleveland Clinic Foundation, Cleveland, Ohio, USA

2008-2009 Senior Research Scientist, CUBRC, San Francisco, CA - Buffalo, NY, USA .

2005-2008 Postdoctoral Visiting Fellow, Brain Tumor Institute, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, USA

2000-2005 Postdoctoral Visiting Fellow, Molecular Pathology Section, Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, NIH, Rockville, Maryland, USA

1999-2000 Research Scientist, Laboratory of Molecular Biology of Bacterial Pathogens, Cell and Molecular Microbiology Division, Institute of Microbiology, Czech Academy of Sciences, Czech Republic.

1994-1998 Staff Scientist, Laboratory of Developmental Genetics, Institute of Cytology and Genetics, Novosibirsk, Russia.

1992-1994 Junior Scientist (Ph.D. Fellow), Laboratory of Developmental Genetics, Institute of Cytology and Genetics, Novosibirsk, Russia.

Research Background

Design and development of assays for identification of novel anti-cancer and anti-viral products:

1. Design and development of gene-specific chromatin accessibility assay for high throughput applications to discover epigenetically active anti-cancer drugs.

2. Design and development of a High-throughput plate assay for detection of intact viral capsids in the cell-free system in the absence of antibodies.

3. Design and development of small molecule screening approach in co-culture cell system.

4. Design and implement a variety of cell-based assays, apoptosis, proliferation, adhesion, migration/invasion, reporter (luciferase) assays.

5. Design and development of method for direct detection of micro-RNAs (siRNA) targets in cytoplasm. Strong experience in gene silencing technologies

-Neoplastic transformation assays (in vivo and in vitro). Establishing and executing in vivo cancer models for evaluating anti-tumor efficacy.

-Establishment, cultivation, enrichment and characterization of ES (stem) cell lines from embryos of the American mink (Mustela vision) and mouse. Genotyping and subcloning.

-experienced handling of laboratory animals (rodents)

-Immunofluorescence techniques: immunostaining, FACS BD-caliber (four colors), microscopy. Image processing and analysis.

-Transfection of cells and analysis of transient transfectants. (Calcium phosphate, electroporation, lipofection and transduction with retroviruses). Establishment, culturing and analysis of stably transfected clones.

-Molecular methods: DNA/RNA/protein purification from tissues and cultured cells; Southern, Northern, Western blots; PCR, RT-PCR, Taqman analysis, Pyrosequencing, SSCP, methylation sensitive PCR, chromatin-immunoprecipetation technique (CHIP), EMSA. Two-phase partitioning of cells and biomolecules.

-cell-free approach for viral nuclear-capsid protein synthesis and assembly into intact capsid

-Gene cloning and expression analysis (5’, 3’-RACE based on adapter ligation or TdT polyA tailing, restriction, ligation, isolation and analysis of recombinant DNA clones, sequencing, data analysis).

-advanced knowledge in biochemistry, analytical methods, and organic chemistry (university level).

-More then 20 years of Tissue culture experience (primary, immortalized, cancer; human, mouse, rat, chicken, etc.). Establishment of primary cell culture from mouse embryo or tissues of adult mice.

Cultivation and characterization of normal human neural and skin progenitor cells.

-Proficiency in computer applications (Ms Windows, Mac OS, Linux) such as MS Word, Excel, PowerPoint, Lasergene 6, Vector NTI, SciFinder, etc.; multiple graphics programs. Molecular visualization and modeling. Solid mathematical and statistical background. Competence in biological databases.

-familiarity with patents, licenses and materials transfer agreements in biotechnology

-Over 20 years of proven experience.

-Competent and diverse skills in areas of molecular biology.

-Disciplined in technical expertise with detailed knowledge of various protocols.

-Skillful in problem formulation and solving with keen observational and intuitive abilities.

-Flexible and resourceful with excellent verbal, written and presentation skills as well as proficiency in several computer applications.

-Capable of providing insightful interpretation of experimental data and training of personnel in instrumentation and troubleshooting.

-Smart, enthusiastic, optimistic and dependable with the ability to excel in challenging and competitive environments.

Teaching activities

Scientific supervisor of a diploma research of graduate students at the Department of Cytology and Genetics, Faculty of Natural Sciences, Novosibirsk State University, Russia.

Supervision / instruction for neurosurgery residents during a research rotation at Brain Tumor Institute, The Cleveland Clinic Foundation.

Research Interests

micro RNA biogenesis, Ribozyme based, RNA dependent RNA/DNA polymerase, Stem cells, Epigenetic in cancer, normal development, regeneration and aging. Chromatin modification and gene imprinting. Small molecule screen

BIBLIOGRAPHY

1. Vatolin S, Weil RJ. Chapter 17. Extension of endogenous primers as a tool to detect micro-RNA targets. Methods Enzymol. 449:357-71. 2008

2. Vatolin S, Navaratne K and Weil R. A novel method for detection of functional microRNA targets. Journal of Molecular Biology 358 (4): 983-996. 2006

3. Hong JA, Kang Y, Abdullaev Z, Flanagan PT, Pack SD, Fischette MR, Adnani MT, Loukinov DI, Vatolin S, Risinger JI, Custer M, Chen GA, Zhao M, Nguyen DM, Barrett JC, Lobanenkov VV and Schrump DS Reciprocal binding of CTCF and BORIS to the NY-ESO-1 promoter coincides with derepression of this cancer-testis gene in lung cancer cells. Cancer Research 65(17): 7763-7774. 2005

4. Vatolin S, Abdullaev Z, Pack SD, Flanagan PT, Custer M, Loukinov DI, Pugacheva E, Morse H, Schrump DS, Risinger JI, Barrett C, Lobanenkov VV. Conditional expression of CTCF-paralogous transcriptional factor BORIS in normal cells results in de-methylation and de-repression of MAGE-A1, and reactivation of other cancer testis genes. Cancer Research, 65(17): 7751 - 7762. 2005

5. Docquier F, Farrar D, D'Arcy V, Chernukhin I, Robinson AF, Loukinov D, Vatolin S, Pack S, Mackay A, Harris RA, Dorricott H, O'Hare MJ, Lobanenkov V, Klenova E. Heightened expression of CTCF in breast cancer cells is associated with resistance to apoptosis. Cancer Research. 2005 Jun 15;65(12):5112-22.

6. Loukinov DI, Pugacheva E, Vatolin S, Pack SD, Moon H, Chernukhin I, Mannan, Poonam M, Larsson E, Kanduri C, Vostrov A, Cui H, Niemitz EL, Rasko JEJ, Docquier FM, Kistler M, Breen J, Quitschke W, Renkawitz R, Klenova EM, Feinberg AP, Ohlsson R, Morse HC III and Lobanenkov VV. BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma. Proc Natl Acad Sci U S A. 99(10):680*-**-****.

7. Rasko JE, Klenova EM, Leon J, Filippova GN, Loukinov DI, Vatolin S, Robinson AF, Hu YJ, Ulmer J, Ward MD, Pugacheva EM, Neiman PE, Morse HC 3rd, Collins SJ, Lobanenkov VV. Cell growth inhibition by the multifunctional multivalent zinc-finger factor CTCF. Cancer Research, 61(16):6002-7 2001.

8. Vatolin S.Y., Okhapkina E.V., Matveeva N.M., Shilov A.G., Baiborodin S.I., Philimonenko V., Zhdanova N.S. and Serov O.L. Scheduled perturbation in DNA during in vitro differentiation of mouse embryo-derived cells. Mol. Reprod. Dev., 47:1-10 1997.

9. Vatolin S.Y., Okhapkina E.V., Zhdanova N.S. and Serov O.L. Time-course of changing in DNA single strand breaks during in vitro differentiation of murine pluripotential embryonic cells // Dokl. Akad. Sci. Rus., 348:834-***-****. (In Russian).

10. Sukoyan M.A., Vatolin S.Y., Golubitsa A.N., Zhelezova A.I., Semenova L.A., and Serov O.L. Embryonic stem cells derived from morulae, inner cell mass and blastocysts in mink: comparisons of their pluripotencies // Mol. Reprod. Dev., 36:148-***-****.

11. Sukoyan M.A., Golubitsa A.N., Zhelezova A.I., Shilov A.G.,Vatolin S.Y., Maximovsky L.P., Andreeva L.E., McWhir J., Pack S.D., Bayborodin S.I., Kerkis A.Y., Kizilova H.I., and Serov O.L. Isolation and cultivation of blastocyst-derived stem cell lines from American mink (Mustela vison) // Mol. Reprod. Dev., 33:418-***-****.

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