Assistant Research

Mary L. Savari Dhason

*** ****** ***** *****

Chesterfield, MO

Tel: 636-***-****(residence) 812-***-**** (mobile)

E-mail: **********@*****.***

Immigration Status: U.S. Permanent Resident

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Education & Training

2007-2012 Research Associate, Department of Biology, Indiana University

2004- 2007 Research Assistant, Department of Biology, Texas A&M University

2002-2004 Research Assistant II, Division of Infectious Diseases, School of Medicine, Vanderbilt University

1990-1992 M.Sc. in Chemistry, St. Joseph’s College, Bharathidasan University, India

1987-1990 B.Sc. in Chemistry, Holy Cross College, Madurai Kamaraj University, India

Scientific Skills:

Biochemistry and Molecular Biology

• Protein expression and purification

• SDS PAGE, Western blotting, EMSA and other biochemical assays

• Antisense mRNA probe synthesis- In vitro transcription

• DNA preparation, restriction enzyme digestion and DNA sequencing

• Preparation of competent cells and transformation into bacterial cells

• PCR, RT PCR and DNA cloning

• In situ hybridization and immunolocalization

• ELISPOT assay

General Lab Skills

• Design, conduct and analyze experimental data in support of various research projects

• Maintenance of research equipment and stock solutions

• Train undergraduate/graduate students and other lab personal in research techniques and use of equipment

• Procurement and inventory of chemicals and other supplies

• Disposal of hazardous/Bio waste

• Lab Safety protocol and maintaining records

• Ordering Lab Supplies

Experience with Software Packages

Microsoft Office Suite (word, excel, power point), Adobe photo shop

Instrumentations/Equipments Handled

ÄKTA (FPLC), HPLC, UV-Spectrophotometer, SDS PAGE and Agarose gel electrophoresis, PCR, Hemocytometer, centrifuge machines and pH meter

Research Publications

1. Mary S. Dhason, Josep C. Y. Wang, Adam Zlotnick. (2012) Differential assemble of Hepatitis B Virus core protein on single- and double stranded nucleic acid correlate with the viral life cycle.

2. Porterfield, J. Z., Dhason, M.S., Nassal, M., Stray, S. J., and Zlotnick, A. (2010) Full length hepatitis B virus core protein packages viral and heterogeneous RNA with similarly high levels of cooperativity. J.Virol, 84(14),7174-84.

3. Millimaki, B.B., Sweet, E.M., Dhason, M.S. and Riley, B.B (2007) Zebra fish athoh1 genes: classic proneural activity in the inner ear and regulation by Fgf and Notch. Development, 134, 295-305.

4. Drake, WP., Dhason, M.S., Nadaf, M., Shepherd, B.E., Vadivelu, S., Hajizadeh, R., Norman, L.S. and Kalams, S.A. (2007) Cellular recognition of mycobacterium tuberculosis ESAT-6 and KatG peptides in systemic sarcoidosis. Infect Immun, 75, 527-530.

References

Available upon request

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