Technician Development

*** *. **** **, *** *

Philadelphia, PA ***** 267-***-****

kfgv3k@r.postjobfree.com

Kristin Marie Daumer

Objective

To break into a career of scientific/technical writing, using my academic and pharmaceutical experiences and knowledge to enhance my desire to learn this exciting and dynamic field.

Education

B.S., Biology, St. Joseph’s University, Philadelphia, PA, Sep 2002

M.S. Biomedical Sciences, Thomas Jefferson University, Philadelphia, PA, Jul 2008

Thesis: Using Caveolin-Mimic Peptides to Affect Caveolin-1 Regulation In Vivo.

Work Experience

March 2012 - Present Wuxiapptec, Philadelphia PA

Associate Scientist, Virology Operations

Performing virus based assays in a GLP setting, testing client material for viral contaminants, utilizing in vitro and in vivo assays, including hemagglutination and hemadsorption. Adhering to GLP standards, documentation, and SOP guidelines.

June 2011 – March 012 Analytical Biological Services at Merck, North Wales, PA

Cell Culture Scientist, Assay Development

Maintaining, culturing, and banking various cells lines. Preparing both small scale cell deliveries for assay development and large scale deliveries for robotic high throughput screening. Experience in delivering both live cell deliveries in suspension and deliveries from frozen cell lines, as well.

March 2010 – June2011 GlaxoSmithKline, Kind of Prussia, PA

GMP Operator for Fermentation Operations, Clinical Activity Supplies

Scale up cell cultures from vial thaw to 100 L reactor inoculation. Thaw, maintain, and grow cell cultures using sterile technique. Sample of cell cultures from large scale bioreactors. Revise, read, and follow SOPs, record data . Adhering to GMP standards, documentaion, and SOP guidelines.

July 2004 - March 2010 Thomas Jefferson University, Philadelphia, PA

Research Assistant, Department of Cancer Biology

Researched therapeutic treatment for atherosclerosis through inducing a knock out phenotype in a wild-type mouse using Caveolin-mimic peptides to affect Cav-1 function in vivo. Isolated mammary fibroblasts and observed morphology via whole mount and BrdU incorporation using INK4 KO mice as a means to study breast cancer. Executed assays using Fast Protein Liquid Chromatography FPLC) to interpret and analyze results. Routinely performed animal handling and surgeries.

Research Technician, Department of Anatomy, Pathology, and Cell Biology

Observed the expression of PKC isoforms involved with myelination by cell culture. Studied the effects of ethanol on differentiating oligodendricytes and Myelin Basic Protein (MBP) expression by western blot, immunofluorescence, and confocal microscopy.

Oct 2002 - June 2004 The Lankenau Hospital, Wynnewood, PA

Research Technician, Lankenau Institute for Medical Research

Identified and characterized a mutation in the hamster UV-1 cell line that causes a defect in DNA repair. Performed PCR, mammalian and bacterial DNA isolations, bacterial transformations and mammalian tissue culture.

Related Course Work

Bioanalytical Techniques Toxicology

Extracellular Matrix Proteins Laboratory Animal Science

Immunology Pathology

Technical and Computer Skills

Tissue Culture, Western Blotting, PCR, ELISA, DNA Isolations, Transformations, Immunofluorescence, Immunohistochemistry (IHC), FACS

MS Windows, PowerPoint, Excel, Word, Outlook, Access, SAS

Certifications

Aseptic Technique qualification, April 2010

White Belt Certification, Feb 2011

Publications

Frank PG, et al. Role of caveolin-1 in the Regulation of Lipoprotein Metabolism. Am J Physiol Cell Physiol. 2008 Jul;295(1):C242-8.

Sotgia F, et al. Loss of Caveolin-3 induces a Lactogenic Microenvironment That Is Protective Against Mammary Tumor Formation. Am J Pathol. 2009 Feb;174(2):613-29.

Bonuccelli G, et al. Caveolin-1 (P132L), a Common Breast Cancer Mutation, Confers Mammary Cell Invasiveness and Defines a Novel Stem Cell/Metastasis-Associated Gene Signature. Am. J. Pathol. 2009 174: 1650-1662.

Mercier I, et al. Genetic Ablation of Caveolin-1 Drives Estrogen-Hypersensitivity and the Development of DCIS-Like Mammary Lesions. Am. J. Pathol. 2009 174: 1172-1190.

Sotgia F et al. Caveolin-1-/- Null Mammary Stromal Fibroblasts Share Characteristics with Human Breast Cancer-Associated Fibroblasts. Am. J. Pathol. 2009 174: 746-761.

Sotgia F et al. Tumor cells induce the cancer associated fibroblast phenotype via caveolin-1 degradation: Implications for breast cancer and DCIS therapy with autophagy inhibitors. Cell Cycle. 2010 Jun 12;9(12)

Trimmer C., et al. CAV1 inhibits metastatic potential in melanomas through suppression of the integrin/Src/FAK signaling pathway. Cancer Res. 2010 Oct 1;70(19):7489-99.

Martinez-Outschoorn UE et al. Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells. Cell Cycle. 2010 Aug 15;9(16):3256-76.

Llaverias G, et al. A Western-type diet accelerates tumor progression in an autochthonous mouse model of prostate cancer. Am J Pathol. 2010 Dec;177(6):3180-91.

Llaverias G et al. Role of cholesterol in the development and progression of breast cancer.

Am J Pathol. 2011 Jan;178(1):402-12.

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