E-mail address: kaqbjl@r.postjobfree.com

Position: M.S in Chemistry and Biochemistry, UCSD.

Advisor: Pieter Dorrestein

Education:

**/****-**/**** ******** Sun Yat-Sen University, Kaohsiung, Taiwan

Bachelor of Science in Biological Science

Total Credits Earned: 141 G.P.A: 3.49

9/2010-current University of California, San Diego

Master of Science in Department of Chemistry and Biochemistry

Thesis: MS/MS networking guided genome mining unsequenced bacteria through association with publicly available genome.

Honors & Certifications:

Interdisciplinary program in Biotechnology Certificate (37 credits)

Issued by National Sun Yat-Sen University

Elected as 2008 Model Student of Department of Biological Sciences.

Master in Chemistry and Biochemistry, UCSD.

GRE General: Math: 800/ Verbal: 630. Total Score: 1430

Extracurricular Activity:

03/2005 – 06/2008 School Representative Team of Tennis at National Sun Yat-Sen Uni.

Represent the university participate in inter-university competitions.

09/2004 – 06/2008 Badminton Team Member of Biological Science Dept, Sun Yat-Sen Uni.

Represent the department participate in on-campus competitions.

03/2005 – 06/2008 Biology Summer Camp at National Sun Yat-Sen University

Voluntary Counselor

Area of Interest

Molecular Biology, Biochemistry, Biotechnology and Genetics.

Working Experience:

2007-2008 Microbiology & molecular biology

Undergraduate research project Analyze the diversity of coral-associated bacteria with techniques of PCR-DGGE

This study applies two molecular biologic techniques, polymerase chain reaction (PCR) and gradient gel electrophoresis (DGGE) and use 16S rDNA as the objects to analyze the importance of coral-associated bacteria on the health and growth of corals. It further compares the DNA sequence obtained from PRC-DGGE with the database of 16S rDNA, identify the species of coral-associated bacteria, and construct the microbe phylogenetic tree so as to determine the microbe compositions and variations of healthy and ill corals.

2010-2012 Microbiology & Mass spectrometry

During interaction between bacteria, some interesting metabolites can be observed by imaging mass spectrometry. After observing interesting ions, we further use HPLC and tandem mass spectrometry to investigate them. In addition, most secondary metabolites come from nonribosomal peptides synthase (NRPS). We combine mass spectrometry and bioinformatics tool to help us identify nonribosomal peptides.

2012 Teaching assistant

Teaching Assistant (organic chemistry lab), Department of Chemistry & Biochemistry, University of California, San Diego

Instrument skills

1 Matrix assisted Laser Desorption Ionization- Time of Flight (MALDI-TOF) (mass spectrometry: autoflex and microflex)

2. Fourier transform mass spectrometry (FTMS)

3. quadrupole ion trap mass spectrometer (ITMS)

4. Nanospray desorption electrospray ionization (nanoDESI)

5. High performance Liquid chromatography (HPLC)

6. Cytoscape

Publications:

2010-2012 :

1. Inter-kingdom metabolic transformations captured by microbial imaging mass spectrometry. Wilna J. Moree, Vanessa V. Phelan, Cheng-Hsuan Wu, Nuno Bandeira, Dale S. Cornett, Brendan M. Duggan, Pieter C. Dorrestein;PNAS (2012).

2. NanoDESI based MS/MS network guided mapping for genome mining of non-ribosomal peptide families produced by unsequenced microbes through association with publicly available sequencing data. Don D. Nuygen1,2, Cheng-Hsuan Wu1,2, Wilna J. Moree1,2, Anne Lamsa3, Marnix Medeema4, Marystella Aparicio5, Librada Atencio5, Javier Ballesteros5, Joel Sanchez5, Ronnie Gavilán5, Jaime Martinez5, Jeramie Watrous2, Xiling Zhao2, Vanessa V. Phelan2, Corine van der Wiel2,6, Roland Kersten7, Harold Gross8, Rene de Mot9, Elizabeth A. Shank10, Pep Charusanti11, Brendan Duggan6, Joe P. Noel12, Bradley S. Moore7, Nuno Bandeira6, 13, 14, Bernhard Ø. Palsson11, Kit Pogliano3, Marcelino Gutiérrez*,5, Pieter C. Dorrestein*,2,6,7, 15

( manuscript in preparation, first co-author)

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