Manager Technician

Dev Singh

**** ******** ******

Naperville IL ***63

Cell:708-***-****

e-mail: ********@*****.***

Education:

University of Hisar (CCS HAU-Hisar, India):1991-1995

PhD Bio- Chemistry

Career Summary:

• Goal oriented professional with strong analytical skills as demonstrated by scientific background.

• Possess extensive experience in Organizational leadership, supervising, designing, execution, and analyzing in vivo and in vitro studies in bio-science research leading to 30 publications and 20 research papers presented at International seminars and conferences.

• Development and review of protocols and critical review of scientific literature in support of scientific studies.

• Extensive experienced in the area of protein biochemistry/ enzymology, stem cell research, and molecular biology related studies/techniques, especially in developing and implementing protein/enzyme purification/characterization and assay development as well as enzyme kinetics.

• Currently working as Scientist in a reputed Pharmaceutical Company and have earlier industrial working experience of more than three years as Manager Research and Development in ISO certified Pharmaceutical Company (Arbro Pharmaceutical Ltd, India).

• Proficiency with molecular biology techniques, gene knockouts, in vivo animal model studies (Mice, Rat, Sheep and Pig models).

• Innovative mindset with great ability and willingness to learn and implement novel techniques, tools and methods. Knowledge of complex biochemical pathways and a working knowledge of advanced molecular biology and strain development techniques.

• Excellent written and communication skills as demonstrated by publications and presentations and grants submitted for federal funding.

Research team management including scientific training to graduate, under graduate students, technician and research analyst/ Post-doctoral fellows.

Research and Laboratory Experience Summary:

Biophysical techniques for characterization proteins and protein aggregates and formulation development of biological molecules and applying new biophysical tools to accelerate the protein stability studies.

Expert in designing vector constructs (Vector construction), various protein expressions in E. coli and baculovirus (prokaryotic recombinant protein expression system) as well as over expression of eukaryotic proteins in vivo system then purification and characterization of these proteins.

Expert in generating transient transfected cells including mammalian and generating stable cell lines for expression of target proteins.

• Expertise in handling small laboratory animals

• Familiar with setting up and running biochemical assays (protein, glycogen, enzyme assays, etc.), running ex vivo primary tissue assays and with aspects of protein analysis (SDS-PAGE gels and western blotting). Ex vivo culture of arteries and veins.

• Enzyme/ Protein purification using various chromatographic techniques including affinity, ion exchange, size exclusion (SEC), FPLC, hydrophobic interaction (HIC), reverse-phase chromatography gel filtration, immuno- precipitation, IEF gel and protein analysis using FTIR/IR, Raman Spectroscopy, differential scanning calorimeter (DSC), dynamic light scattering analysis of proteins and aggregates, nano-sight technology. Extensive experience working with HPLC, GC and GCMS. Strong familiarity with protein characterization techniques: SDS-PAGE, Western blot, ELISA, and other biological techniques as well as analysis of post-translational modifications of proteins. Legend –protein binding assays radio-immuno assays as well as in vivo interactions of various proteins. Expert and knowledge about basic principles involves in protein biochemistry as well as well familiar with principles of scale down and scale up procedures for protein purification.

• Capable of keeping at the abreast of protein purification technology. Extensive experience in mentoring, coaching, training and administration of performance management to others (Graduate students, lab Technicians, post and pre-doctoral fellow as well laboratory technician.

• Working knowledge as per GMP/GLP/GDP guide lines. And expert in drug assays as per various pharmacopeia (IP, BP, USP). Stability testing of drugs as per ICH guide lines. Experience in cGMP for Quality Control and experience with FDA audits. Method validation following ICH guidelines.

• Legend –protein binding assays radio-immuno assays as well as in vivo interactions of various proteins. Knowledge and experience with spectroscopic techniques and instrumentation including fluorescence spectroscopy. Published more than 10 research papers in high impact peer review journals on various proteins/ enzyme purification and their characterization. (See the publication list).

• Working knowledge and troubleshooting experience in the following techniques/ Instrument Equipment: NanoSight, FPIA, HIAC low volume accusizer, HPLC, IR, GCMS, phosphor-image analysis, flow cytometery, anisotropy assays, immuno-histochemistry and various types of electrophoresis.

• Extensive experience in techniques used in cell culture for the production of proteins after cloning in vitro and vivo with specific tags for purification and characterizations. Excellent working experience with various cell lines including CHO, 3T3, pre-adepocytes, HEK, HEPG2, BAECSs, HAECs, Smooth muscle cells, fibroblast cells and various primary cells. Good understanding and appreciation for upstream cell culture manipulations (eg, viral transduction, recombinant gene transfection, selection, cloning) and downstream processes (product detection, recovery, characterization). Excellent time management skills and ability to work on multiple projects.Working knowledge in 2D and 3D cell culture using collagen, metrigel and other matrices..

• Working knowledge of recombinant DNA techniques including designing and making new construct, working knowledge of designing various primers, interpretation of the data and familiar with various tools to clone the cDNAs and then express the encoded proteins using bacterial expression vectors. siRNA studies to lower down specific targets.

• Managing the Laboratory, contacting vendors, evaluating the products and placing the orders for laboratory. Trouble shooting day to day problems faced in the laboratory.

• Mentors to undergraduate students/ graduate students, pre-doctoral/post-doctoral fellows as well training to laboratory technician and research assistant scientist.

Work Experience Summary:

Hospira, Inc August 2011 to Present

Global Pharma Biologics R&D

Scientist

• Analyze stability data for potential trends and provide statistical data reports: collect, manipulate, and interpret trends using simple and multiple regression analysis such as ANOVA, Linear and Non-Linear Regression.

• Initiate analytical testing/investigations/re-testing such as OOS and OOT to confirm noncompliance.

• Developing biochemical methods for biological tissues: study design, implementation of analytical methods, and final reporting.

• Technical elements of developing new methods for determining purity, impurity, composition, and mechanical properties of biologic products

Stability testing:

• Conduct in-house sensory panels. Design and perform special protocols for: sensory evaluations; photo stability; temp excursion; freeze/thaw; and bulk hold/ shipping, etc.

• Modify laboratory stability testing protocols to effect changes to MOAs and test limit specifications.

Stability protocol and report generations:

• Oversee development protocols designed to evaluate product's chemical and physical integrity over time, and substantiate or extend a provisional expiration dating period.

• Guide support staff in the preparation of International stability reports

Stability database evaluation and assessment:

• Maintain a compliant, audit ready LIMS stability data base. Perform data-entry and verification of data.

• Utilize computer software (EXCEL or SLIM) in manipulation of data, reporting and approving test results.

• Directly involved in characterization of proteins and protein formulations. Method transfer protocols/ SOP auditing GMP/GLP/GDP work and differentiate GMP work from non GMP work and absolute requirement to follow GMP procedures.

• Extensive work with SDS-page, Western Blot and IEF in addition to other protein characterization methods

• Prepare and maintain SOPs. Prepare protocols/reports/regulatory filing documents.

• Identify and facilitate continuous improvement to the Stability Program.

• Assure adherence of staff to cGMP stability practices in the execution of all tasks.

University of Illinois, Department of Pediatrics July 15th 2010 to August 2011

Research Assistant Professor

• Role of PKG and LZ+ MYPT1 in regulation of contractile forces under hypoxia in mice model.

• Exploring the cell signaling pathways in Fetal Pulmonary arterial smooth muscle cells exposed to hypoxia and studying the vasoconstriction and vaso-relaxation using various drugs to overcome hypoxic effect in vivo model.

University of Illinois: Department of Medicine: July 1st 2004- July 15, 2010

• Isolation of Primary endothelial cells from the lumen of femoral, coronary and carotid arteries from pigs fed on high and low cholesterol and then study the Kir Current.

• Isolation of MSH cells from undifferentiated Bone Marrow derived cells. And results have shown that kir current is regulated by cholesterol.

• Developed techniques for the study of vasodilatation of small arteries and developed ex-vivo cultures of coronary arteries and transecting the arteries with adeno and lenti-viruses.

• Isolation of Primary endothelial cells from the lumen of femoral, coronary and carotid arteries from pigs fed on high and low cholesterol and then study the Kir Current.

• Isolation of MSH cells from undifferentiated Bone Marrow derived cells. And results have shown that kir current is regulated by cholesterol.

University of Kentucky: Pharmaceutical Sciences: Nov 2002 to May 2004

Research Associate

• Cloned and purified Human Supernatant Protein factor (SPF) and Rat Supernatant protein factor- like protein (SPF2), Squalene monooxygenase and P-450 reductase in E. coli and studied the regulation of Cholesterol.

University of Kentucky, Lexington: Department of Surgery: Nov 1999 to May 2002

Post-Doctoral Fellow

• Behavioral Testing procedures (Beam walk and Morris Water Maze) in rats, Traumatic Brain Injury: Biochemical, Histological, Pharmacological Aspects,

• Small animal surgeries and dissection (Rats). Alcohol feeding to rats Liquid diet method and Intubations method (Gavages) for acute treatment. Biochemical analysis of wide spectrum of metabolites and metabolic pathways after traumatic brain injuries.

ARBRO Pharmaceuticals Ltd

Manager Research and Development: New Delhi (India): August 1996- Nov 1999

• Industrial working experience of approximately three years as Manager Research and Development in ISO certified Pharmaceutical Company (Arbro Pharmaceutical Ltd, India).

• During my stay at Arbro Pharmaceuticals Ltd (ISO certified company) as Manager Research and Development, I trained the work force as per GLP/GMP and other regulations; various advanced techniques used in protein purification as well as well familiar with principles of scale down and scale up procedures for protein purification.

• With my efforts and expertise (my major achievement), Arbro Pharmaceutical company was accredited by National Board of Laboratories Accreditation (India).

Professional References:

• Professor (Dr) Subbaiah PV: Department of Medicine Section Endocrinology and Metabolism, BD 909, University of Illinois at Chicago. MC-797, 835 S Wolcott Ave Chicago IL-60612-7340. Phone 312-***-****. Fax:312-***-****. E-mail: ********@***.***

• Professor (Dr) Porter Todd D: Department Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536-0082 Phone: 859-***-**** Fax: 859-***-**** E-mail: *******@***.***

• Dr Sanoj K. Suneja, Ph.D. Research Program Analyst, National Institute on Aging, Office of Extramural Affairs, 7201 Wisconsin Avenue, Gateway Bldg., Suite 2C218, Bethesda, MD 20892-9205, Phone: 301-***-**** and 301-***-****.Fax: 301-***-****, Email:*******@****.***.***

•

Total number of lectures/ presentations in national and international conferences: 20.

Total number of publications: 29

List of Publication

1. Qiwei, Y, Lu Z., Singh, D.K. and Raj, J.U. (2012). Inhibition of histone lysine methyltransferase G9a blocks cell proliferation, migration, and contractility in ovine fetal pulmonary arterial smooth muscle cells (Cell Prol-0981-11). Cell Prolif. 45(4):335-44.

2. Shentu, TP, Singh, D.K., Cho, M, Subbaiah, PV and Levitan, I. 2012. The role of oxysterols in control of endothelial stiffness. J. Lipid Res. 53(7):1348-58.

3. Singh, D.K., Sarkar, J., Raghavan, A., Reddy, S. and Raj, J.U. 2011. Hypoxia modulates the expression of Leucine Zipper positive MYPT1 and its interaction with protein Kinase G and Rho Kinases in pulmonary arterial smooth muscle cells. Pulm. Cir. 1(4):487-98.

4. Singh, D.K., Shentu, T.P., Enkvetchakul, D. and Levitan, I. 2011. Cholesterol regulates prokaryotic KIR channel by direct binding to channel protein. Biochim. Biophys. Acta (Biomembrane): 1808(10):2527-33.

5. Tzu-Pin Shentu, Igor Titushkin, Singh, D.K., Keith J. Gooch, Papasani Subbaiah, Michael Cho, and Irena Levitan. 2010. OxLDL-induced decrease in lipid order of membrane domains is inversely correlated with endothelial stiffness and network formation. Am. J. Physiol. Cell Physiol. 299 (2):C218-29.

6. Singh, D.K., Rosenhouse-Dantsker, A., Nichols C.G., Enkvetchakul, D. and Levitan, I. 2009. Direct regulation of prokaryotic KIR channels by cholesterol. J. Biol. Chem. 284 (44): 30727-36.

7. Subbaiah, PVS, Sowa, JM and. Singh, D.K., 2008. Role of sphingolipids in cellular cholesterol homeostasis: Effect of ceramide on HMG CoA Reductase activity and cholesterol trafficking. Arch. Biochem. Biophys. 474:32-8.

8. Subbaiah, PVS, Sowa, JM and. Singh DK, 2008. Role of sphingolipids in cellular cholesterol homeostasis: Effect of ceramide on HMG CoA Reductase activity and cholesterol trafficking. Arch. Biochem. Biophys. 474:32-8.

9. Singh, DK and Subbaiah, PV. 2007. Sphingolipids and Inflammation: Modulation of the activity and fatty acid specificity of group X Secretory phospholipase A2 by sphingomyelin and ceramide. J. lipid Research 48(3):683-92.

10. Singh, DK, Gesquiere LR and Subbaiah, PV. 2006. Regulation of the activity and fatty acid specificity of group V secretory phospholipase A2 by sphingomyelin and ceramide. Arch. Biochem. Biophys. 459(2):280-287.

11. Singh, D.K., Li, Li and Porter TD. 2006. Policosanol inhibits cholesterol synthesis by activation of AMP kinase. J. Pharmacology and Experimental Therapeutics, 318 (2): 1020-1026.

12. Singh D.K., and Porter, TD, 2006. Inhibition of sterol 4 -methyl oxidase is the principal mechanism by which garlic decreases cholesterol synthesis. J. Nutr. 136(3):759S-64S.

13. Mokashi, V., Singh, D.K., and Porter, T.D. 2005. Supernatant Protein Factor Stimulates HMG-CoA Reductase in Cell Culture and in vitro. Arch Biochem Biophys. 433(2):474-80.

14. Mokashi, V., Singh, D.K. and Porter, T.D. 2004. Rat Supernatant Protein factor-like protein (SPF2) Stimulates Squalene mono oxygenase and activated by protein kinase A. Biochem. Biophys. Res. Comm. 316: 688-692.

15. Singh, D.K., Mokashi, V., Elmore, C.L., and Porter, T.D. (2003) Phosphorylation of supernatant protein factor enhances its ability to activate microsomal squalene monooxygenase. J. Biol. Chem. 278: 5646-5651.

16. Dong, G., Singh, D.K., Dendle, P. and Prasad. R.M. 2001.Regional expression of Bcl-2 mRNA and mitochondrial cytochrome C release after experimental brain injury in the rat. Brain Research. 903(1-2): 45-52.

17. Prasad, R.M., Doubinskaia, I, Singh, D.K., Dhillon, H.S., Campbell, G., Mace, D., Fletcher, A., Dendle, P., Yurek, D.M., Scheff, S.W. and Kraemer, P.J. 2001. Effects of binge ethanol administration on the behavioral outcome of rats after lateral fluid percussion brain injury. J. Neurotrauma. 18(10): 1019-1029.

18. Singh, D.K., Malhotra, S.P. and Singh, R. 2000. Purification and characterization of cytosolic pyruvate kinase from developing seeds of Brassica (Brassica campestris L.) Indian J. Biochem. Biophys. 37(1): 51-58.

19. Singh, D.K. and Singh, R.A. 2000. Helicobacter pylori induced peptic ulcer and its treatment (Review Article). The Antiseptic. 97(3): 73-75.

20. Singh, D.K. and Singh, R.A. 1999. Estimation of Lansoprazole in pharmaceuticals dosage form by high performance thin layer chromatography. The Eastern Pharmacist 499:113-116.

21. Singh, D.K. and Singh, R.A. 1999. Effect of pH on the dissolution profile and comparative in vitro dissolution studies of commercially available sparfloxacin tablet dosage form. The Eastern Pharmacist 499:178-186.

22. Singh, D.K. and Singh, R.A. 1999.Simultaneous estimation of Oxybenzone and Octylmethoxy cinnamate by Gas Liquid Chromatography. The Eastern Pharmacist. 494:121-122.

23. Singh, D.K., Singh, R.A. and Anand, A. 1998. Simultaneous estimation of camphor, menthol, methyl salicylate and Thymol by Gas liquid chromatography in pharmaceuticals preparations. Indian Drugs 35(5): 300-302.

24. Singh, D.K. and Goel, S. 1998. Dissolution profile (in vitro studies) of commercially available Norfloxacin tablets. The Eastern Pharmacist. 488:119-121.

25. Singh, D.K., Malhotra, S.P. and Singh, R. 1998. Purification and characterization of Plastidic pyruvate kinase from developing seeds of Brassica (Brassica campestris L.) Indian J Biochem. Biophys. 35 (6): 346-352.

26. Singh, D.K., Singh, R.A. and Anand, A.1998. Dissolution (in vitro) studies of Ibuprofan and paracetmol in combined tablets dosage forms. The Eastern Pharmacist. 486:113-115.

27. Dhamija, S.S., Gera, R., Singh, D.K., Singh, D. and Marchant, R. 1997. Physiological and biochemical characterization of inter generic hybrid of thermo tolerant and non-thermo tolerant yeasts. J. Basic Microbiol. 37(5): 307-312.

28. Singh, D.K. and Singh, R. 1996. Partial purification and characterization of phosphoenol pyruvate phosphatase from developing seeds of Brassica (Brassica campestris L). Proc. Nat. Acad. Sci. 66 (spl. Issue): 53-63.

29. Singh, D.K. Rao, A.S., Singh, R. and Jambunathan, R. 1988. Amino acids composition of major seed storage proteins of promising chickpea (Cicer arietinum L.) cultivar. J. Sci. Food Agric. 43: 373-379.

Editorials, Reviews, Book Chapters:

1. Singh, D.K. and Singh, R.A. 2000. Helicobacter pylori induced peptic ulcer and its treatment (Review Article). The Antiseptic. 97(3): 73-75.

2. Singh, D. K., Shentu, T.P. and Johnson, T. 2012. Cholesterol: Biosynthesis, functional diversity, homeostasis and regulation by natural products. Biochemistry / Book 1 ISBN 979-953-307-484-8 Book edited by: Deniz Ekinci (Invited book Chapter).

Papers under preparations:

1. Yulia Epshtein, Balaz Ordog, Kunal Gursahani, Singh, D.K., John W. Christman, Stanley Nattel and Irena Levitan. (2012). Kir channels are essential for oxLDL-induced bacterial phagocytosis in Bone-Marrow Derived Macrophages. Under preparation.

Total number of lectures/ presentations in national and international conferences: 20.

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