Laurelis

LAURELIS E. SANTIAGO

**** ** **** ** **** ***-**

Homestead, FL 33035

Tel: 787-***-****

E-mail: *********@*****.***

Objective

Contribute to senior technical operations scientist-54369 (Posting ID: 20090124JB16489646F) position by applying molecular biology and medical biotechnology knowledge, teaching and research skills developed through research experience in fertility and stem cell research.

Education

University of Massachusetts, Amherst - MS in Animal Biotechnology and Biomedical Science. 2006 – 2008 Courses: Cell, Genes & Development, Biochemistry, & Developmental Biology.

University of Puerto Rico, Bayamón, 2000-2005. BS in General Biology, Magna Cum Laude.

Courses: Chemistry, Organic Chemistry, Molecular & Cell Biology, & Human Physiology.

Experience

• Molecular Biology Internship, Northeast Alliance Program, University of Massachusetts, Amherst, MA, 06/2006 - 03/2007. Research Title: "Effect of Perchlorate on thyroid hormone mediated brain development". Perchlorate can reduce thyroid hormone synthesis by blocking iodide uptake, which is essential for thyroid hormone synthesis. Analysis of perchlorate function in offspring leads to a prediction that children in some areas in the world are at developmental risk because of perchlorate contamination.

• Undergraduate Research Project. Department of Organic Chemistry, University of Puerto Rico at Rio Piedras, 08/2004 - 12/2004. Research Title: “Self-Assembly of Guanosine Analogs in Aqueous Media”. As part of degree program, at UPR Rio Piedras Campus in August 2004, conducted organic chemistry research on self-assembly of guanosine analogs in aqueous media. Synthesized-8-Ar-guanosine analogs that interact with G-quadruplex to cause stabilization, which in turn inhibits telomerase enzyme. Telomere shortening has implications for resistance to certain cancers and other diseases.

• Undergraduate Internship Summer Research, University of Illinois, Urbana-Champaign, IL, 06/2004 - 08/2004. Research Title: “Differential Expression of Cyclins During Decidualization”. Analysis of the expression of cyclins D3, A, B and E during the phase of decidualization leads to a greater understanding of a factor which can contribute to the avoidance of early pregnancy loss and infertility.

• Undergraduate Internship Summer Research, University of Illinois, Urbana-Champaign, IL, 06/2003 - 08/2003. Research Title: “The effect of estrogen in mouse testis determination”. Determined whether estrogen can inhibit the SRY genes, which are responsible for controlling the sex of the embryo, by treating the mice embryo with estrogen.

• University of Puerto Rico at Bayamón PR, 08/2002.

Assisted freshmen students in chemistry lab sessions and lecture review sessions.

Seminars, Workshops, Training University of Wisconsin, Madison, 09/2005. Safety Dept. Earned certificate for training on working safety with chemicals. The training satisfies 29 CFR part 1910.1450 (f) Employee Information and Training.

University of Massachusetts, Amherst, 10/2004. Attended Seventh Annual Symposium of the Center for Neuroendocrine Studies. This seminar presented the opportunity to learn about neuroendocrinology in specific areas of research topics such as molecular implications at the behavioral level, brain development, sexual differentiation, environmental endocrine disruptors, energy balance, and reproductive functions and behaviors.

University of Puerto Rico at Rio Piedras, 01/2003. Participated in monoclonal antibody laboratory workshop.

Skills

Molecular Biology Techniques:

• Gel electrophoresis

• Polymerase Chain Reaction (PCR)

• Real Time PCR

• Reverse Transcriptase PCR

• Plasmid Construction and DNA cloning

• In Vitro Transcription

• In Situ Hybridization

• RNA Extraction

• Chemical Solution

• MicroArray Organization and Analysis

• DNA isolation (minipreps)

Histology Techniques:

• Embedding

• Sectioning (Ovary, testis, Brain)

• Stain Ammion for embryo sex identification

• Pre-implantation embryo (Stem cells) handling

• Whole-mount & Tissue Section Immunocytochemistry

• Hematoxylin and Eosin Staining

Laboratory Instruments & Animal Handling

• Spectrophotometer & Incubators

• Converted Microscope

• Rotary Microtome (Used for Paraffin wax sectioning)

• Genotyping

• Breeding

Computer: MS Office, Mac Vector, BLAST

Languages: Written and oral Spanish and English.

References

Dr. Humphrey Hung-Chang Yao, University of Illinois, Urbana-Champaign.

Contact: *****@****.*** Tel. 217-***-****

Dr. Miriam Quiñones, University of Puerto Rico, Bayamón.

Contact: *********@****.***: Tel. 787-***-**** ext. 3278

Dr. Jesse Mager, University of Massachusetts, Amherst.

Contact: ******@*****.*****.*** Tel. 413-***-****

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