RESUME

NAME. SURESH R. PAI. Ph.D., D.A.M., M (ASCP)

ADDRESS. 601 Lincoln Court Avenue NE

Atlanta, GA 30329-1818

Tel.: Home 281-***-**** Cell: 281-***-****

E-mail:ixqfk8@r.postjobfree.com

RESIDENTIAL STATUS. US Citizen.

UNDERGRADUATE EDUCATION.

1974: Bachelor of Science [Microbiology, Chemistry] University of Bombay, India.

1979: Diploma in Administrative Management, Bajaj Institute of Management Studies, University of Bombay, India.

POSTGRADUATE EDUCATION.

1977: Master of Science [Medical Microbiology], University of Bombay, India.

1983: Ph.D. (Medical Microbiology) “Studies on drug resistance in typed strains of

Pseudomonas aeruginosa” University of Bombay, India.

Jan. 94 - Nov. 95 American Academy of Microbiology's Postdoctoral Training in Clinical

Microbiology and Public Health. Div. of Laboratory Medicine, Clinical Microbiology,

University of Texas M.D. Anderson Cancer Center, Houston, TX. Eligible for Board Certification-Diplomate in Microbiology.

ACADEMIC APPOINTMENTS (& Responsibilities)

-May 19, 2008 – Present Senior Environmental Scientist, Atlanta Research and Education Foundation (AREF), Div. of Parasitic Diseases, Centers for Diseases Control and Prevention, 4770, Buford Highway NE, Mail Stop F-36, Atlanta, GA 30341-3724

-April 16, 2006 –Dec. 21, 2007 Lead Microbiologist, Public Health Federation of Employers (PHFE) @ City of Houston Lab Services, 1115 South Braeswood, Houston, TX 77030

-July 27, 04-April 15, 06 Lead Microbiologist, Centers for Disease Control & Prevention @ City of Houston Lab Services, 1115 South Braeswood, Houston, TX 77030

-Feb. 03 - July 23, 04 Laboratory Supervisor, Molecular Diagnostic Section, Department of Health and Human Services, Houston, TX.

-July 00 – Jan. 03 Research Associate, Dept. of Infectious Diseases, UT Health Science Center, Houston, TX.

-Oct. 97 – July 00. Research Scientist, Dept. of Pathology and Laboratory Medicine,

University of Texas Health Science Center, Houston, TX.

-Aug. 96 – Oct 97. Research Associate, Dept. of Pathology, Baylor College of Medicine, Houston, TX.

-Dec. 95 - June 96. Biological Scientist IV, Head of Molecular Microbiology Unit, Dept. of Health and Rehabilitative Services, Jacksonville, FL.

-Jan. 94 - Nov. 95. Postdoctoral fellow in Clinical Microbiology and Public Health (American Academy of Microbiology's CPEP-approved). Div. of Laboratory Medicine, Clinical Microbiology, University of Texas M.D. Anderson Cancer Center, Houston, TX.

-June 89 - Jan. 94. Postdoctoral fellow, Dept. of Pathobiology, School of Veterinary Medicine, Auburn University, Auburn, AL.

-April 88 - May 89. Research Associate, Biomedical Research, Alabama State University, Montgomery, AL.

-Feb.86 – Mar. 88. Postdoctoral Fellow, Division of Biological, Environmental

and Medical Research, Argonne National Laboratory, Argonne, IL.

-Aug. 78 - Dec. 85. Lecturer in Microbiology, King Edward Memorial Hospital, Bombay, India.

-Jun. 74- May 78. Research Assistant, Molecular Microbiology, King Edward Memorial Hospital, Bombay, India.

LICENSURE. M (ASCP)

Specific Technical Expertise:

Clinical Microbiology:

Bench-level clinical Microbiology, Clinical immunology, mycology, parasitology, mycobacteriology, virology, antimicrobial susceptibility testing, sterility testing, standard operating procedures (SOP’s), quality control, quality assurance, drug combination studies, infection control, epidemiology-Restriction Fragment Length Polymorphism (RFLP) to subtype strains of Mycobacterium tuberculosis complex, Pulse field gel electrophoresis (PFGE), infectious diseases, laboratory management, molecular clinical microbiology, tissue culture and flow cytometry, time kill experiments and invitro mouse model studies, working knowledge of a BSL-3 environment and chain-of custody regulations.

Recombinant DNA Technology:

Plasmid and chromosomal DNA isolation, RNA isolation, cloning, DNA sequencing, agarose and polyacrylamide gel electrophoresis (SDS-PAGE), electro elution, dot blot, polymerase chain reaction (PCR), Real time PCR, Real Time RT-PCR, uptake of antisense oligonucleotides, mobility shift-DNA-binding assays, monoclonal antibody production, western, southern and northern blots, ELISA, outer membrane protein isolation, protein expression, purification, and characterization.

Computer Experience: Microsoft Word, Excel and PowerPoint.

HONORS:

08/30/2007: Award of Excellence to the Houston BioWatch Laboratory Team of which Suresh Pai was the Lead Microbiologist, BioWatch Exercise and Evaluation Program, 2006.

10/02/2004: Certificate of Appreciation to Suresh Pai from the United States Department of Homeland Security in recognition of outstanding contribution to the successful deployment of the BioWatch monitoring system.

10/02/2004: Certificate of Appreciation to the City of Houston Public Health Laboratory from the United States Department of Homeland Security in recognition of outstanding contribution to the successful deployment of the BioWatch monitoring system. Suresh Pai was the Lead Microbiologist,

PUBLICATIONS.

ABSTRACTS

1. Smith. C.M. Pai S. R and Hill V. Comparison of Ultrafilters for recovery of Biothreat agents from 100-L tap water samples. ASM BioDefecse and Emerging Infectious Diseases Meeting. Baltimore, MD, Feb. 2009

2. Pai SR BioWatch actionable results- Four years in retrospective, BioWatch National Meeting, Chicago, IL, May 2007

3. Pai SR, Singh KV and Murray BE In vivo efficacy of a ketolide ABT-773 against enterococci in a mouse peritonitis model 41st ICAAC, Chicago, IL Dec, 2001.

4. Pai SR, Actor JK, Hunter RL and Jagannath C Rapid detection of latency of Mycobacterium tuberculosis in mouse organs100th Gen. Meet. ASM, Los Angeles, CA, May 2000.

5. Pai SR, Actor JK, Hunter RL and Jagannath C. Identification of viable and non-viable

M. tuberculosis in mouse organs by directed RT-PCR for antigen 85B mRNA. Ann meet. Ass Mol Path, St. Louis, MO, 1999.

6. Jagannath C, Actor JK, Sepulveda E, Pai SR, and Hunter RL. Poloxomer CRL-1072 enhances the antimycobacterial effects of human and murine macrophages through nitric oxide and chemokine secretion.39th Gen.meet ICAAC, San Francisco, CA, 1999.

7. Pai SR, Actor JK, Hunter RL and Jagannath C. Mycobacterium tuberculosis down-regulates IL-8 production in human U937 macrophages early during infection. 99th Gen. meet. ASM, Chicago, IL, 1999.

8. Jagannath C, Copenhaver R, Armitige L, Pai S, Wanger A, Norris S, Actor JK, and Hunter RL. A mutant of Mycobacterium tuberculosis H37Rv deficient in antigen 85A expression acts as vaccine against experimental tuberculosis in mice. 99th Gen. Meet. ASM, Chicago, IL.1999.

9. Jagannath C, Armitige L, Pai S, Wanger A, Norris S, Actor JK, and Hunter RL. Altered growth characteristics and vaccinogenic effects of a Mycobacterium tuberculosis H37Rv mutant deficient in antigen 85A expression. Inter Sym on TB vaccine Develop.

& Evaluation, San Francisco, CA, 1998.

10. Pai SR, Actor JK, Hunter RL and Jagannath C. Expression of antigen 85 complex proteins in smooth transparent and smooth opaque variants of Mycobacterium avium. 98th Gen. Meet. ASM, Atlanta, GA, 1997.

11. Pai SR, Esen N, Pan X, and Musser JM. Routine rapid Mycobacterium species assignment based on species-specific allelic variation in the 65-kilodalton heat shock protein (hsp65) gene. 97th Gen. Meet. ASM, Miami. FL, 1997.

12. Pai SR, Tarrand J, and Rolston K. Effect of drug combinations on vancomycin resistant and sensitive enterococci. 95th Gen. Meet. ASM, Washington, D.C., 1995.

13. Clarridge J.E., Pai, S. R., Hashemi, F. B., C. Wright., and Webb, D. Workflow, benefit and cost study of three nucleic acid amplification (NAA) methods for the diagnosis of tuberculosis. 95th Gen. Meet. ASM, Washington, D.C., 1995.

14. Tarrand J., Pai, S. R., Buckner M., and Rolston K. Demonstration of in-vitro antagonism between aztreonam and third generation cephalosporin and imipenem. 95th Gen. Meet. ASM, Washington, D.C., 1995.

15. Bird, R. C., and Pai, S. R. Overexpression of c-fos, under control of the SV40 promoter complex, induces overexpression of Rb in transfected cells. Am Soc Cell Biol. Denver, CO, 1992

16. Bird, R. C., and Pai, S. R. c-fos antisense oligonucleotide produces a cell cycle block during exponential cell proliferation. Am Soc Cell Biol. Boston, MA, 1991.

17. Pai, S. R., and Bird, R. C. Growth of HeLa cells cotransfected with plasmids containing c-fos gene under the control of the SV40 promoter complex, pRSVcat, and G418 resistance. Research Emphasis Forum, Phi Zeta, The Honor Society of Veterinary Medicine. Auburn, AL.1989.

18. Pai, S. R., and Bird, R. C. Growth of HeLa S3 cells cotransfected with plasmids containing c-fos gene under the control of the SV40 promoter complex, pRSVcat, and G418 resistance. Am Soc Cell Biol. San Diego, CA, 1990.

19. Pai, S.R., Upshaw, Y., and Singh, S. P. Characterization of monoclonal antibodies to the outer Membrane proteins of S. typhimurium. 89th Gen. Meet. ASM, New Orleans, LA,1989.

20. Kubitschek, H.E., and Pai, S. R. Pool sizes in E. coli THU during cell growth. 87th Gen. Meet. ASM, Atlanta, GA,1987.

21. Pai. S.R., and Kubitschek, H. E. Pool size during the cell cycle of E. coli. 87th Gen. Meet.

ASM, Atlanta, GA,1987.

22. Pai, S. R., and Joshi, L. Characterization of drug resistance plasmids of Ps. aeruginosa. Ind. Assoc. Med. Microbiol. Ann. Meet. Solapur, India, 1983.

23. Pai. S.R., and L.Joshi. Aeruginocin typing of Ps. aeruginosa on the basis of low and high molecular weight aeruginocins. Maharashtra Chap. Conf., Ind. Assoc. Med. Microbiol., India, 1983.

24. Pai, S. R. , and Joshi, L. Postoperative infections following open heart surgery and their drug resistance pattern. Assoc. Microbiol. Ind., Madurai, India, 1977.

25. Pai, S. R., Joshi, L., and Parulkar, G. B. Bacterial and fungal infections following open heart surgery. 1st Conf. Surgery in the Tropics, Bombay, India, 1976.

REFEREED ORIGINAL ARTICLES IN JOURNALS

1. Pai SR, Singh KV and Murray BE. In vivo efficacy of a ketolide ABT-773 against enterococci in a mouse peritonitis model.Antimicrob. Agents Chemother.47:2706-2709, 2003.

2. Nannini EC, Pai SR, Singh KV and Murray BE. Activity of tigecycline (GAR-936), a novel glycylcycline, against enterococci in the mouse peritonitis model. Antimicrob. Agents Chemother. 47:529-532, 2003.

3. Pai SR, Actor JK, Sepulveda E., Hunter R, Jagannath C. Identification of viable and non-viable Mycobacterium tuberculosis in mouse organs by direct RT-PCR for antigen 85B mRNA. Microbiol Pathogen. 28:335-342, 2000.

4. Jagannath C, Pai S , Actor JK and Hunter RL. CRL-1072 enhances antimycobacterial activity through interleukin-8. J. Interferon Cytokine Res., 19:67-76, 1999.

5. Pai SR, Esen N, Pan X. and Musser J. Routine rapid Mycobacterium species assignment in a clinical microbiology laboratory based on species-specific allelic variation in the 65-kilodalton heat shock protein (hsp65) gene. Arch Pathol Lab Med.,121:859-864, 1997.

6. Pai SR, and Bird RC. Interaction of Rb tumor suppressor protein with the c-fos promoter in c-fos transfected cells over expressing c-fos and Rb. Anticancer Res. 17:3265-3272, 1997.

7. Pai SR, and Bird RC. Overexpression of c-fos induces expression of the retinoblastoma tumor suppressor gene Rb in transfected cells. Anticancer Res.14:2501-2508, 1994.

8. Pai SR, and Bird RC. c-fos expression is required during all phases of the cell cycle during exponential cell proliferation. Anticancer Res. 14:985-994, 1994.

9. Kemppainen BW, Brock M, Terse P, White J, Vodela J, Zurovac O, Pai SR, Bird RC. Evaluation of altered chemical sensitivity in cells overexpressing c-fos as an assay for chemicals causing birth defects. Princeton Sc. Pub. Co., Inc. NJ. 606-615, 1994.

10. Pai SR, and Bird RC. Growth of HeLa S3 cells cotransfected with plasmids containing

c-fos gene under the control of the SV40 promoter complex, pRSVcat, and G418. Biochem. Cell Biol. 70:316-323, 1992.

11. Pai SR, Upshaw Y. and Singh SP. Characterization of monoclonal antibodies to the outer membrane protein (OmpD) of S. typhimurium. Canad. J. Microbiol. 38:1102-1107, 1992.

12. Pai SR, and Kubitschek HE. Catabolic pools in Escherichia coli. Res. Microbiol. 143:173-181, 1992.

13. Pai SR, and Bird RC. Quick and high efficiency electroelution of nucleic acid fragments. GATA. 8:214-216, 1991.

14. Pai SR, and Kubitschek HE. The phosphate pool in E. coli. FEMS Microbiol. Lett. 81:49-52, 1991.

15. Kubitschek HE, and Pai SR. Activation of amino acid transport during steady state growth of Escherichia coli. Res. Microbiol. 140:517-530, 1989.

16. Kubitschek HE, and Pai SR. Variation in precursor pool size during the division cycle of Escherichia coli: Further evidence for linear cell growth. J. Bacteriol. 170:431-435, 1988.

17. Pai SR, and Joshi LJ. Typing of Pseudomonas aeruginosa on the basis of low and high molecular weight aeruginocins. Postgrad. Med. 31:52-56, 1985.

18. Pai SR, and Joshi LJ. Drug resistant plasmids of Pseudomonas aeruginosa. Ind. J. Med. Res. 80:521-527, 1984.

REFERENCES: Available upon request

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