Lab Manager, research associate

Venus tan

***** **** *** **

Seattle, WA *****

425-***-**** (cell)

********@*****.***

Research Associate/ Manager

Experience in both academic and industrial environments with strengths in aseptic techniques, protein purification, communication and problem-solving. An enthusiastic individual who is goal motivated, team-task oriented, a quick learner and likes to train others. Graduated from University of Washington, Seattle in 2003 with a Bachelor of Science in Cellular and Molecular Biology. Flexible with schedule and willing to travel extensively. Strong desire to foster business relationship between departments, company branches and vendors.

Lab Skills

Mammalian cell culture, Transfection, Electrophoresis, ELISA, SDS-page, Western/Dot Blot, Column Chromatography, Protein Purification, DNA extraction, Multi-tasking, Organizational skills, Teaching, Purchasing, Networking, Flow Cytometry, Aseptic Techniques, Whole Blood Processing, Phlebotomy, PCR amplification, HLA typing.

Work Experience:

Benaroya Research Institute, Lab Manager & Research Technician II Oct 06- Present

Performed investigative work for research projects using Flow Cytometry, produced five different lines of MHC class II molecules and two associated antibodies, carried out non-routine work. Maintained facilities and purchase equipment and supplies.

• Tracked immune responses of six subjects pre and post flu shots for two years.

• Saved the lab $21,547 with discounts and free goods in eight months.

• Phlebotomy-trained and HLA typed prospective subjects. Arranged schedule and drew subjects’ blood for five other lab members on top of own duties.

• Safety officer for two years. Prepared the lab for fire department inspection.

Xcyte Therapies, Inc, Bothell Washington, Cell Manufacturing Associate I. July 04- May 05

Manufactured and cultured T-cells using aseptic techniques in cGMP environment, wrote official reports and maintained facilities. Assisted Quality Control, Clinical Affairs and Process Development department in validating process and equipment. Troubleshoot process and equipment failure.

• Performed installation and operational qualifications (IOQ) on four equipment, wrote nine final reports and revised eleven Standard Operating Procedures (SOP) and forms.

• Participated in intra and inter-departmental meetings by contributing ideas, writing of meeting minutes and taking initiative to solve problems on agenda. Conducted interview for two potential manager candidates and provided feedback to management.

• Cross- trained in Material Controls on receiving and handling of goods. Prepared for and corrected issues on Quality Assurance audit. Negotiated and discussed issues with suppliers.

• Active learner: Passed cGMP certification in two days, passed clean room gowning and personnel aseptic qualification in first round, obtained performance appraisal goal of being trained in less than a year.

Publications/Abstracts

Yang J, Gebe JA, Huston L, James E, Tan V, Yue BB, Nepom GT, Kwok WW. H5N1 strain-specific hemagglutinin CD4+ T cell epitopes restricted by HLA DR4. Vaccine 2009 Jun 12;27(29):3862-9 Epub 2009 May 3.

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