Project Plant

Location:

United States

Posted:

June 12, 2012

Contact this candidate

Resume:

SANTOSH KUMAR. R. JADHAV

Room no –****, PTC Department,

Biochemical Sciences Division, +912*-********

National Chemical Laboratory, M +919*********

Dr. Homi bhabha road, Pune - 411 008. **********@*****.***

Profile: Ph. D with 4 years of research experience in molecular biology, proteomics & Bioinformatics.

Degrees / Fellowships

Ph. D. (2011) Biotechnology University of Pune Maharashtra

M. Sc. (2001) Biotechnology Bharathidasan University Tamil Nadu.

B. Sc. (1999) B.Z.C S.V. University Andhra Pradesh

CSIR-SRF (2007) CSIR – Senior Research Fellow ship

Professional Experience

Project Assistant III Since April 2010 National Chemical Laboratory PUNE INDIA

Guide: Dr. B. M. Khan, HOD, PTC division.

Project: “Pathway engineering and system biology approach to homologous expression of phytoceuticals”.

• Homologous expression of artemisinin, morphine and withanolides.

• Saponin glycosyltransferase gene from W.somnifera.

• Present partial gene was identified, isolated, cloned, characterized and expressed.

Senior Research Fellow April 2007 – April 2010 National Chemical Laboratory PUNE INDIA

Guide: Dr. B. M. Khan, HOD, PTC division.

Thesis title: “Molecular studies on Glycosyltransferases from Withania somnifera”.

• Isolated flavonoid and saponin glycosyltransferase gene,

• Heterologous expression and characterization of the enzyme.

• Enzyme kinetics studies, modelling and docking studies.

• Tissue specific expression studies of the gene using real time PCR.

Project Assistant II Nov 2004 – April 2007 National Chemical Laboratory PUNE INDIA

Guide: Dr. S.K. Rawal, HOD, PTC division.

Project: “Biotechnological approaches for the improvement of plant species to pulp and paper”.

• Worked on lignin biosynthesis pathway genes.

• Isolation of gene using PCR based approach and cDNA library screening method.

• Using heterologous probe to screen the cDNA and gDNA library of Leucaena leucocephala.

• Standardizing agrobacterium mediated transformation of Leucaena leucocephala.

Project Assistant II June 2004 – Nov 2004 National Chemical Laboratory PUNE INDIA

Guide: Dr. Rajini S. Nadgowda, HOD, PTC division.

Project: “Improvement of tropical trees (Mangifera indica) using tissue culture techniques.

• Development of protocols for the micro-propagation.

• Standardization of Somatic embryogenesis in Mangifera indica.

Product specialist executive Jan 2003 – May 2004 Life technologies India Pvt ltd CHENNAI INDIA

• Worked as a molecular biology grade product executive.

M. Sc. Dissertation work Jan 2001-April 2001 National Chemical Laboratory PUNE INDIA

Guide: Dr. Vidya S. Gupta, HOD, PTC division.

Project: “Identification of molecular markers linked to leaf rust resistance genes in wheat (Triticum aestivum L)”. As a part of the above-mentioned ISCB (Indo Swiss Collaboration in Biotechnology) project, molecular analysis was performed to identify the putative PCR based molecular markers (RAPD, ISSR) linked to leaf rust resistance gene Lr23 with a set of NILs developed to generate the mapping populations.

Skills acquainted

Molecular biology

• Conversant with nucleic acid isolation and associated analytical skills (PCR; Southern, Northern & Western blotting; cDNA & gDNA library prep & Screening; Genome walking etc.).

• Nucleic acid quantification with Spectrophotometer and Bio-analyzer.

• Competent in gene cloning and transformation.

• Experienced in quantitative gene expression analysis (Real time PCR).

Plant Tissue Culture

• Hands on plant tissue culture techniques and agrobacterium transformation.

Biochemistry

• Experienced in protein expression, isolation and Purification (AKTA - FPLC, Ni-NTA & Ion exchange).

• in vitro enzyme kinetic assays.

• Competent in protein electrophoresis including 2D electrophoresis for differential analysis.

• Conversant with HPLC, MALDI, LC-MS and ELISA.

Bioinformatics

• Experienced in literature and sequence data mining from public databases and analysis.

• Proficient in multiple sequence alignments and phylo-genetic analysis.

• Conversant with protein homology modeling and docking.

Publications

1. Somesh singh, R. J. Santosh Kumar, R.K. Vishwakarma, Ruby, B.M. Khan* (2011). “Functional characterization of flavonoid glycosyltransferase gene from Withania somnifera.” (Accepted Molecular biology reports).

2. Neha Gupta, Poonam Sharma, R. J. Santosh Kumar, R. k. Vishwakarma and B. M. Khan* (2011). “Functional Characterization and differential expression studies of Squalene Synthase from Withania somnifera” (Accepted Molecular biology reports).

3. R. J. Santosh Kumar, Krunal patel, Ruby, Somesh singh, R.K. Vishwakarma, B.M. Khan* (2012). “Functional and Structural Characterization of a Glucosyltransferase Specific to Flavonoid 7-O-Glucosides from Withania somnifera” (Submitted to Molecular Biotechnology).

4. Trupti kad, R. J. Santosh Kumar, Bashir khan, Nadaf Altafhusian (2012). “Is functional BADH2 present in fragrant Pandanus amaryllifolius Roxb?.” (Submitted to Plant Cell Reports).

5. Ruby, R. J. Santosh Kumar, R.K. Vishwakarma, Somesh singh, B.M. Khan* (2011). “Molecular cloning, characterization and expression analysis of glycosyltransferase gene from Bacopa monniera” (Submitted to Plant Cell Reports).

Abstracts / Book chapter/ Presentations

• Book chapter published in the book “Genetic Engineering”, ISBN 978-953-308-81-5, titled “Genetic Engineering of Phenylpropanoid Pathway in Leucaena leucocephala”. Bashir Khan*, S.K. Rawal*, Manish, sushim .G, sameer .S, Noor .M, Arun .Y, Pallavi .K, Abhilash O.U, Santosh .G, Sumita, Rishi, Somesh, R.J. Santosh Kumar, Prashant .S, Parth, Shakeel and Kannan.

• “Isolation and Characterization of novel glycosyltransferases from Withania somnifera”. R. J. Santosh Kumar, Ruby, Dr. B.M. Khan*. Abstract of the poster presented in the National Symposium on Plant Propagation, Conservation, Modification and Characterization organized at IHBT, Palampur (2009).

• “Secondary metabolic flavonoids glycosyltransferases from Withania somnifera”. R. J. Santosh Kumar, Ruby, Somesh singh, R.K. Vishwakarma, Dr. B.M. Khan*. Abstract of the poster presented in the International Symposium on Current status and Opportunities in Aromatic and Medicinal Plants held at CIMAP (2010).

• “Steriospecific glycosylation of medicinally important compounds”. Somesh singh, R. J. Santosh Kumar, R. K. VIshwakarma, Ruby, Dr. B. M. Khan*. Abstract of the poster presented in the 1st Chemical Research Society of India (CRSI) Zonal meeting held at National Chemical Laboratory (NCL, 2011).

Personal details

• Date of birth: 05/07/1979; Male; Indian.

• Languages Known: Hindi, English, Marathi & Telugu.

• Received National Cadet Corps “C” certificate from Andhra battalion 30 in April 1999.

References

1. Dr. B. M. Khan,

Head, Plant Tissue Culture Department,

Biochemical Sciences Division,

National Chemical Laboratory,

Pune - 411 008.

Ph +912*-********

Cell +91-988*******