Resume

Sign in

Maintenance Manufacturing

Location:
Jersey City, NJ, 07306
Salary:
negotiable
Posted:
August 15, 2012

Contact this candidate

Resume:

Himaly K. Shinglot

Phone: 201-***-****; Email: HYPERLINK "mailto:ib8qag@r.postjobfree.com" ib8qag@r.postjobfree.com

SUMMERY

* ***** ** ********** ** laboratory involving project on “Effects of metformin on prostate cancer cells”

Hands-on-experience in technics such as Mammalian Cell culture/Tissue culture (on c4-2b, myc-cap, RWPE-1 cell lines), cell line characterization and screening

Proficient in performing assays: ELISA, Western Blot, Gel Electrophoresis, Flow Cytometry (FACS analysis), Cytotoxicity assay, Fluorescence microscopy

Proficient in molecular techniques like Plasmid Purification, DNA Quantification and Transient Transfection, RNA extraction, purification and Quantification

Protein extraction, purification and characterization

Chromatography, centrifugation knowledge, filtration technics for protein purification

Proficient in performing immunochemical and biochemical methods like ELISA, Western Blotting, RT-PCR and Gel electrophoresis

Transfection, plasmid construction and genecloning

Experience in animal handling, sectioning and staining of tissues

Dosing, monitoring and sample preparation

Dosing for metformin effect study on mice

Preparation of different dilutions of metformin

Cell viability assay

Crystal violet

Alamar blue

Dual luciferase assay

Using Plate reader to measure fluorescence

Genotyping

Clip tails

Extract DNA for PCR

Plasmid construction

Gene cloning

Transfection

Animal handling

Mice

Images for Immunohistochemistry (Microscopy)

Detailed Lab book management

Detailed knowledge of GLP and GMP

Writing, reviewing and evaluating SOPs

Lab house keeping and organizing the chemicals and making solutions, measuring pH, autoclaving, making stock, maintaining chemical inventory, chemical waste maintenance

Experience in QA and QC

I have almost one and half year of HPLC experience (theoretical and practical)- During my undergrad study, I had HPLC experiments and theory classes for 2 semesters. And I underwent a practical training on HPLC in LOCOST manufacturing industry.

Maintaining detailed research data, data interpretation

Maintaining, ordering lab inventory

6 months of work in QA/QC department

SKILLS

Formulation: 1+ years of experience in tablets and capsules manufacturing and characterization (physical testing such as diameter, disintegration, dissolution), purity test such as HPLC

Molecular Biology: BCA assays, Gel electrophoresis, Western Blot, ELISA, Plasmid preparation, cDNA synthesis, SDS-PAGE and PCR.

Tissue Culture: Development and Maintenance of cell culture, GFP transfection studies, Cytotoxicity studies, Transfection

Analytical: HPLC, UV spectrophotometer, Dissolution Test, Disintegration test

Fluorescence microscopy, Flow Cytometry (FACS analysis), pH meter

Instrumentation: Synergy-HT Biotek Plate Reader, Coulter Counter, Nano Drop, Ultrasonicator, tablet press, homogenizer

Animal Handling: tissue sectioning and staining, SC/IV injection, cardiac puncture, neck dislocalization, ear-tagging, clip tails

Detailed knowledge of GLP and GMP

Maintenance and updation of SOP

Lab house keeping and organizing the chemicals and making solutions, measuring pH, autoclaving, making stock, maintaining chemical inventory, chemical waste maintenance

Computer skills:

ChemDraw

Protein Data Bank

PDB (Protein Data Bank) viewer

Power point

Excel

Word

EDUCATION

POLYTECHNIC INSTITUTE OF NEW YORK UNIVERSITY, NEW YORK, NY

MAY 2012

MASTER OF SCIENCE IN BIOTECHNOLOGY (GPA: 3.7)

COURSES: BIOCATALYSIS, IMMUNOLOGY, MATERIALS IN MEDICINE, BIOSENSORS, HEALTH CARE AND PHARMACEUTICAL INDUSTRIES, MOLECULAR BIOLOGY, CELL BIOLOGY

THE MAHARAJA SAYAJIRAO UNIVERSITY, BARODA, INDIA

MAY 2008

BACHELOR OF SCIENCE IN PHARMACY (GPA: 3.5)

COURSES: PHYSICAL PHARMACY, DRUG MANUFACTURING AND DISPENSING, PHARMACOLOGY, PHARMACEUTICAL CHEMISTRY, MEDICINAL CHEMISTRY, PHARMACOGNOSY, ANALYTICAL CHEMISTRY, BIOCHEMISTRY

RESEARCH EXPERIENCE (Currently working since March 2011)

Graduate Research Assistant in NYU College of Dentistry, New York, NY

Advisor: Dr. Xin Li

My duties include cell culture, RNA/DNA extraction, purification and quantification, perform PCR array to make cDNA and amplify it, protein assay, western blot, ELISA, gel electrophoresis and SDS-PAGE, flow cytometry, animal handling. Maintenance of detailed lab notebook and inventory

To maintain GMP and GLP

ACADEMIC EXPERIENCE

PARUL PHARMACY INSTITUTE: WORKED AS A DEMONSTRATOR (MARCH 2009- JANUARY 2011)

Teaching practical aspects to the students of Pharmacology and Pharmaceutics

Maintaining SOPs for all the available apparatus in the lab such as dissolution machine, disintegration machine, sonicator

INDUSTRIAL EXPERIENCE

LOCOST MANUFACTURING (Baroda, Gujarat, India): WORKED AS AN INTERN (JUNE 2008-NOVEMBER 2008)

Formulation of various solid and liquid oral dosage forms like tablets, suspensions, emulsions using tablet press and homogenizer.

Pre-formulation studies for the dosage forms.

Determination of the stability of the dosage forms by friabilty and hardness test, dissolution and disintegration studies, sedimentation, phase separation and cracking studies.

Purity testing assays such as HPLC

FOOD AND DRUGS LABORATORY (Baroda, Gujarat, India): WORKED AS A TRAINEE DURING BS (MAY 2008- JUNE 2008)

Worked in the departments of Pharmacology, Medicinal Chemistry, Pharmaceutics and Pharmacognosy

I learnt HPLC, Gas chromatography, Thin Layer Chromatography, titration, measuring effects of various drugs such as acetylcholine on mice and frogs, synthesis, purification and characterization of various medicinal compounds.

EXPERIENCE AS A VOLUNTEER (Baroda, Gujarat, India)

BHASHA TRIBAL ACADEMY: WORKED AS A COMMUNITY PHARMACIST (NOVEMBER 2008- JANUARY 2009)

Dispensing the prescribed medications to the patients and explaining the right usage of the medication

I had to make the patients aware of the over usage of any medications.

SWAMI VIVEKANAND MISSION: WORKED AS A COMMUNITY PHARMACIST (FEBRUARY 2009- MARCH 2009)

Dispensing the prescribed medications to the patients and explaining the right usage of the medication

I had to make the patients aware of the over usage of any medications.



Contact this candidate