KOYUKI TAKENAKA

**** ******** *****, ***. ** • San Diego, CA 92123

619-***-**** • i3gmu7@r.postjobfree.com

RESEARCH ASSOCIATE / ANALYTICAL CHEMIST

Accomplished, self-directed professional with expertise in analytical/bioanalytical chemistry and progressive research experience in both academic and industry settings. Proficient utilizing broad range of laboratory techniques, including LC/MS/MS, GC/MS, and HPLC, to characterize small molecules, peptides, polymers, and proteins. Adeptly support drug discovery through analytical method development; employ both traditional and innovative techniques to develop/validate methods using analytical chemistry knowledge earned from academic research experience. Skilled in accurately tuning, operating, and troubleshooting laboratory instrumentation, and maintaining detailed, comprehensive, records, and ensuring reproducibility of results.

Ensure all actions comply with appropriate regulations/standards; guarantee adherence to GLPs and research lab protocols. Valued and respected associate who thrives as independent contributor and in collaborative team settings. Recognized for diligence, exceptional work ethic, and exemplary performance.

LABORATORY & INSTRUMENTATION SKILLS

Bioanalytical/In-Vitro Assays (DMPK) • Applied Biosystems API 4000 • BioMek FX/NX • HPLC (Shimadzu)

Thermo Finnigan, TSQ Quantum, ESI/APCI • Solid-Phase Extraction • Liquid-Liquid Extraction

GC-MS (Agilent, FID/TIC) with Purge/Trap Autosampler • UV-Vis Spectrometer • pH Meter

GPC, Reverse Phase, Normal Phase, Ion Exchange Columns • Dissolution Tester • ELISA

Polymer, Protein, & Peptide Characterization • Viscometer (Brookfield) • Karl-Fischer • UV Plate Reader

PROFESSIONAL EXPERIENCE

JOHNSON & JOHNSON PHARMACEUTICAL RESEARCH & DEVELOPMENT, San Diego, CA • 2010-Present

Research Associate (On Assignment through Kelly Services/US Tech Solutions, no end date postion)

Selected to support drug discovery initiatives as contract employee of global pharmaceutical preparations manufacturer. Under restrictive deadlines, develop bioanalytical methods (API 4000 LC/MS/MS methods) for approximately 5 compounds per week for DMPK in drug discovery. Perform protein crash and prepare calibration standards/quality controls; establish highly accurate/consistent results for plasma, lung, and brain samples in 96 well plates (automated and non-automated). Utilized rapid equilibrium dialysis device and LC/MS/MS (API 4000, ESI) to screen batched compounds for plasma protein binding.

• Acquired comprehensive knowledge of DMPK assays and gained proficiency troubleshooting matrix effects and ion suppression issues.

• Validated data to assure quality, completeness, and reliability of information and presented results to research scientists and associates in immunology/neuroscience/cardiovascular.

SAN DIEGO STATE UNIVERSITY, San Diego, CA • 2007-2010

Teaching Assistant

Instructed undergraduate laboratory sections for general and analytical chemistry courses while pursuing master’s degree.

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KOYUKI TAKENAKA • Page 2 • i3gmu7@r.postjobfree.com

PROFESSIONAL EXPERIENCE (BEFORE M.S.)

AGAT LABORATORIES, LTD., Grande Prairie, AB, Canada • 2006-2007

Laboratory Analyst

Analyzed small organic compounds in soil and water samples using GC/MS at GLP-regulated, full service testing laboratory and scientific consulting firm. Validated analytical methods by meeting criteria for recovery percentage of untreated samples, controls, and duplicates; determined levels of detection and levels of uncertainties. Prepared external, internal, and reference standards, including calibration checks, matrix spikes, sample spikes, method blanks, surrogates, and retention time markers; authored and revised SOPs and prepared, monitored, and updated quality control data.

• Maintained QC data and monitored data traceability, aiding lab in passing site audits of Canadian Association for Environmental Analytical Laboratories to ensure compliance with GLP regulations.

MACROMED, INC., Acquired by BTG, Sandy, UT • 2002-2004

Analyst I

Performed broad range of laboratory processes and analytical tests in analytical group with private drug development/manufacturing company, specializing in creating new formulations of existing drugs using novel polymeric technologies. Conducted quantitative analyses within non-GLP, preclinical environment; supported formulation studies, in vivo/vitro studies, and stability tests. Utilized HPLC, ELISA, Karl-Fischer, and dissolution tester to complete quantitative analyses and characterization of proteins, peptides, and small molecules in polymer for formulation.

• Played key role in maintaining and troubleshooting variety of equipment and instrumentation, specifically HPLCs, ensuring optimal functioning and guaranteeing reliability and accuracy of data collected.

EDUCATION

Master of Science in Chemistry, emphasis in Analytical Chemistry, San Diego State University – San Diego, CA • 2010

• Thesis: “Method Development for Analysis of Testosterone / Epitestosterone in Human Samples Using LC/MS/MS,” Supervisor: Prof. Dale Chatfield

• Research Focus: Separated testosterone/epitestosterone using C8 column with LC/MS/MS (Thermo TSQ Quantum, ESI/APCI). Developed method for urine sample preparation, enzymatic hydrolysis of steroid conjugates followed by solid supported liquid-liquid extraction. Obtained 10 pg/mL LOD and 50 pg/mL LOQ from standard solutions and reconstituted urine samples with faster, more sensitive method than existing GC/MS/MS methods.

Bachelor of Science in Chemistry, University of Utah – Salt Lake City, UT • 2002

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