Scientist / Manager Scientist

Lily D. Lu

*** ******** ******, ******* *******, PA 19425

Telephone: 856-***-****; E-mail: ********@*******.***

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OBJECTIVE: A MS level dedicated organized team player with excellent analytical and strong trouble shooting skills, greater than 10 years of hands-on experience with in vitro, ex vivo, and in vivo assay development in molecular, cell biology, immunology and oncology is seeking a research scientist position in the biopharmaceuticals or biotech industry.

SKILLS SUMMARY

Molecular Biology:

Cloning, PCR, RT-PCR, Q-PCR & oligonucleotide design, 5’RACE and 3’RACE, cDNA library construction and screening, Northern, Southern, Western blots and in situ hybridization, DNA and protein sequence analysis, siRNA and shRNA technology, genomic DNA and RNA isolation, SDS-PAGE, purification & characterization of recombinant fusion protein, site-directed mutagenesis, microarray.

Cell Biology, Immunology and Oncology

Tissue culture, transient transfections, stable cell line development, ELISA and DELFIA techniques, Luminex assay, T and B cells Elispot, ERK and cAMP assays, Flow Cytometry, Ca+ mobilization using Flexstation, immunoprecipitation and radio-immuno assays, immunohistochemistry, in vitro cytotoxicity and cell proliferation assays, ex vivo and in vivo autoradiography binding, confocal microscopy, urinary protein test.

Pharmacology

Rodent handling including i.p, i.v, p.o and s.c dosing and mouse survival check bleed

WORK EXPERIENCE

Research Associate VI, 2009-Present

Cephalon Inc., West Chester, PA

Immunology- Oncology 2009-present

• Validated major ex vivo and PD biomarker assays, such as cytokine luminex, ELISA, Elispot, FACS and urinary protein test, for SLE mouse model from serum and tissue extraction. Based on our study, a new clinical indication for the drug in Phase II will be initiated. Trained BD LSRFortessa Flow Cytometry Operator.

• Established a novel arthritic paw extraction method for cytokine luminex test to support drug testing in mouse model.

• Established tissue extraction for PK and PD from tumor Xenograft, colon of IBD-CRC model and metastasis lung by using miRNA, luminex and ELISA assays. Dose mice by i.p, p.o, s.c and i.v.

• Performed in vitro caspase-Glo 3/7 assay on cancer cell lines for the drug of interest.

• IVIS® imaging for 4T1, B16F10-Luc and LL/2-Luc cancer cell lines in mice model.

• Established Air Pouch model for inflammation drug testing in rat and mouse

CNS biology 2007-2009

• Administer drug to rats by i.p, p.o, s.c, and i.v for ex vivo and in vivo binding using beta-imager to support in vivo evaluation.

• Established hyperlocomotion and passive avoidance for cognition modeling test.

Sr. Research Scientist I, II, 2001- 2007

Adolor Corporation, Exton, PA

• Cloned targets relevant to pain management for screening and counter-screening.

• Constructed and characterized cell lines for screening and secondary assays.

• Established HTRF cAMP functional assay in 384-well format for proteins of interest.

• Performed DELFIA and ELISA protocols to measure signal transduction events such as receptor internalization, phosphorylation of ERK.

• Measured Ca+ mobilization following activation of Gq-coupled GPCRs and calcium-permeant ion channels, using the Molecular Devices Flexstation.

• Established QPCR methods and analyzed samples from rat and human tissues for CB receptor expression and other markers of changes associated with pain.

• Established RNAi technology to knock down targets of interest by both transient transfection and stable vector-based RNAi transfection.

Research Scientist (level 8), 2000-2001

Rohm & Haas Company, Spring House, PA

• Constructed ecdysone based chemical-inducible gene expression system.

• Analyzed expression clone by protein assay and dual Luc-assay.

Research Associate, 1998-2000

Senesco Technologies Inc., Waterloo, Ontario

• Performed RT-PCR to obtain lipase and DHS fragments with degenerate primers.

• Constructed and screened tomato, arabidopsis, banana, and carnation cDNA libraries.

• Cloned DHS and lipase genes from arabidopsis, banana, carnation and tomato.

• Transformed anti-sense DHS genes into arabidopsis and tomato to investigate their role in plant senescence control.

EDUCATION

Master of Science, Molecular Biology,

University of Waterloo, Waterloo, Ontario

Thesis: Use of Antibodies Raised Against Recombinant Fusion Proteins to Study Protein Turnover in The Endoplasmic Reticulum

Awarded UW scholarship.

Bachelor of Science, Cell Biology,

Northeast Normal University, Jilin, China

COMPUTER SKILLS:

Proficiency in Microsoft Office, PhotoShop, DNAstar, VectorNTI, Prism, Genebank mapping, QuarkXPress, Access 2000, and SigmaStar.

PATENTS

Principal inventor: John Thompson; Michael Wang, Lily D. Lu, Awarded Patent No. 6,538,182 from the U.S. Patent and Trademark Office (the “PTO”) entitled “DNA Encoding A Plant Deoxyhypusine Synthase, a Plant Eukaryotic Initiation Factor 5A, Transgenic Plants and a Method for Controlling Senescence Programmed Cell Death in Plants. March, 2000.

PUBLICATIONS

1. Lily Lu, K. Stump and M. Seavey, “Novel method of monitoring trace cytokines and activated STAT molecules in the paws of arthritic mice using multiplex bead technology”, BMC Immunology Nov. 12; 11:55 (2010).

2. M. Seavey, Lily Lu and K. Stump, Current Protocols in Pharmacology: Animal models of systemic lupus erythematosus (SLE) and ex vivo assay design for drug discovery (Unit 14). Current Protocols in Pharmacology 5.60.5-40, June 2011

3. Lily Lu, K. Stump, N. Wallace, B. Ruggeri, M. Seavey, “Novel, orally active proteasome inhibitor, CEP-18770, protects mice from lupus nephritis with greater tolerability and impact on circulating plasma cell frequencies as compared to bortezomib”. (manuscript in preparation)

4. Lily Lu, K. Stump, B. Ruggeri, M. Seavey, “CEP-33779, an orally active, highly selective, Janus kinase 2 (JAK2) inhibitor depletes plasma cells and reverses active renal disease in mouse models of lupus. (in review – J of Immunology)

5. K. Stump, Lily Lu, B. Ruggeri, M. Seavey, “A highly selective, orally active inhibitor of Janus kinase (JAK2) ablates disease in multiple mouse models of rheumatoid arthritis”. Arhtritis Res Ther. Apr 21, 2011; 13(2):R68

6. H. Schaffhauser, J. Mathiasen, A. Di Camillo, Lily Lu, M, Marino, “Dimebolin is a 5-HT6 antagonist with acute cognition enhancing activities”. Biochemical Pharmacology (2009), 78(8), 1035-1042.

7. M. Wang, L. Lu and J. Thompson, “Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor-5A from tomato”, J. Biol. Chem. 276 (20): 17541-9, (2001).

8. M. Wang, Lily Lu, C. Zhang, J. Thompson, “Pleiotropic effects of suppressing deabidopsis thaliana”, Plant Mol Biol. 52 (6): 1223-35, (2003).

9. M. Padidam, M. Gore, Lily Lu, and O. Smirnova, “Chemical-inducible, ecdysone receptor-based gene expression system for plants”, Transgenic Res. Feb; 12(1): 101-9, (2003).

10. Lily Lu, J. Cassel and E. Mansson, “Alternate splicing of the kappa opioid receptor in human dorsal root ganglia”, Society for Neuroscience, (2002). Abstract# 554.2.

11. Lily Lu, J. Cassel and E. Mansson, “Characterization of ? opioid receptor splice variant KOR4”, Society for Neuroscience, (2003). Abstract# 577.12.

12. Lily Lu, J. Cassel and E. Mansson, “Characterization of two human VR1 splice variants”, Society for Neuroscience, (2004). Abstract#845.5.

13. Lily Lu, C. LaBuda and E. Mansson, “Expression of CB1 and CB2 cannabinoid receptors in rat and human tissues”, Society for Neuroscience, (2005). Abstract#51.1.

14. Lily Lu, G. Stabley, M. Feschenko, E. Mansson and R. Windh, “A comparison of cannabinoid receptor 2 agonists in functional assays”, Society for Neuroscience, (2006). Abstract#791.6.

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