High School Development

Mi-Young Jung, Ph.D.

** **** ******, *********, ************* 02474

508-***-**** | heiz47@r.postjobfree.com |

http://www.linkedin.com/in/miyoungjung

Summary

Assay developer with 7 years of extensive experience in target validation

and screening therapeutic candidates within the field of CNS diseases.

Delivered a deep understanding of target biology and a highly competitive

and innovative early-stage drug discovery portfolio. Known for rapid,

successful resolution of critical issues. Proven leadership and excellence

in initiating projects, motivating colleagues, developing strategies,

implementing state of art technology/knowledge, transferring protocols, and

mentoring lab members.

Technical Skills

. All phases of assay development, including planning, implementation,

and analysis

. Neuronal cultures including Oligodendrocytes, DRG, CGN, cortical and

hippocampal neurons

. High-content screening on the Cellomics platform (neurite outgrowth

assay, OPC differentiation assay)

. Myelination using Oligodendrocyte Precursor Cell (OPC) and DRG

. Functional studies of new proteins using molecular and biochemical

approaches

Experience

BIOGEN IDEC, Cambridge, MA 2005-8/2012

Senior Scientist, Neurobiology (2011-2012)

Scientist II, Neurobiology (2005-2011)

Leading Assay Development for Alzheimer's Disease Programs (GSM, Arc,

antibody therapy)

. Implemented/developed reproducible and ultra-sensitive cell based

assays (MSD, Mesoscale) to detect amyloid peptides 38, 40, 42, and PE

(3-X) using Cho-cell and H4 cells, delivering highly competitive

portfolio in a short time period. These assays were effectively

transferred to colleagues.

. Screened compounds using these assays and monitored biomarkers from

bio- fluids and brain.

. Received recognition for developing reliable assay within a six-month

period.

Implementing New Approaches for Multiple Sclerosis Program (Fumarate)

. Validated the activity of Fumarate on neuroprotection by providing the

evidence that Fumarate activates Nrf2 transcriptional pathway on

astrocytes and oligodentrocytes.

. Led biomarker studies by initiating research, developing protocols,

conducting experiments, selecting and confirming candidates.

. Set up in vitro differentiation and myelination system using OPC and

DRG and developed MSD-based Elisa and high-content screening to

explore target activation.

. Developed reproducible methods to isolate OPCs by shaking and immuno-

panning.

. Worked as a lab mentor and transferred methods to other scientists.

Directing Synaptic Plasticity Programs (Arc, CBLN1, Tetraspanin7)

. Published studies of Arc biology in Cell (the function of Arc as a

modulator in the production of amyloid peptides in Alzheimer's

disease) through collaboration with internal and external scientists.

. Established in vitro binding system using recombinant Arc protein and

HEK cell-expressed Presenilin1.

. Discovered a PS1-derived peptide blocking the interaction of PS1 and

Arc.

. Discovered that Arc phosphorylation loses its binding activity to PS1

NTF.

. Explored CBLN1 and Tetraspanin7 validating the potential function in

synaptic plasticity.

Supporting Regeneration Program (Nogo)

. Supported the team to make accurate decisions on Nogo program by

solving problems in neurite out-growth assay and by providing

consistent data from a different cell type.

. Screened Nogo receptor constructs using neurite out-growth assay

(Cellomics, high-content screening) and survival assay on primary

cultured DRG neurons and cerebella granule neurons.

. Found biomarkers of Nogo receptor application by initiating and

leading biomarker studies.

. Provided understanding of Nogo receptor biology by studying signaling

cascade on Cofilin and Jak2.

. Trained associates and mentored summer interns.

UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL, Worcester, MA 2000-2005

Postdoctoral Fellow, Molecular Medicine

Neuroguidin and Cytoplasmic Polyadenylation Element Binding Protein (CPEB)

Projects

. Studied the function of Midline during the development of spinal cord

and neural crest cell as a translational regulator using CAT reporter

assay, anti-sense morpholino oligo injection, in situ hybridization,

immunohistochemistry, and Western and Northern blottings.

. Established transfection system into neurons and mammalian cell lines

(B104, PC-12, and hela).

. Screened interacting miRNAs and proteins using micro array, cloning,

and co-ip assays.

. Designed mutants and analyzed their phenotypes in in vivo and in vitro

systems.

. Studied signal transduction of NMDA-Aurora kinase-mediated CPEB

phosphorylation in synaptosome using kinase assay and phospho-peptide

mapping.

. Analyzed phenotypes of the brain from CPEB knock-out mice.

. Mentored graduate students.

MOUNT SINAI SCHOOL OF MEDICINE, New York, NY 1995-1999

Ph.D. Candidate, Department of Neurobiology

Developmental Analysis of Mice Lacking Dopamine D2, D3, and D2/D3 Receptors

. Analyzed knock-out mice using Southern, Northern, and Western

blottings and immunoprecipitation.

. Studied the influence of the lack of dopamine receptor subtypes using

HPLC, behavioral analysis, gene expression (c-fos), receptor signaling

cascade analysis (MAPK, and CREB), immunocytochemistry,

histochemistry, and RT-PCR with the application of drugs.

. Trained other graduate students; mentored high school student.

Pre-Doctoral Experience

ROCKEFELLER UNIVERSITY, New York, NY

Research Associate, Immunology Department, Howard Hughes Medical Institute

Interaction among Super Antigen, T cell Receptor, and MHC II

. Characterized interaction domains of super antigen using deletion

studies in insect cell.

. Purified super antigen using FPLC and Histidine-tagging.

CHEIL SUGAR CO., LTD., SAMSUNG, Ichun, South Korea

Procedure Development Scientist, Research and Development Center

HBs Ag and a-Interferon Programs

. Developed purification procedures for pilot scale and patented

procedures and supervised a pilot system.

. Found a method to improve the activity of a-Interferon.

. Established QC procedures and trained technicians.

Professional Memberships

|Society for Neuroscience |American Academy of Neurology |

|Myelin |American Association for the Advancement of |

| |Science |

Education

Ph.D., Neurobiology, Mount Sinai School of Medicine of New York University,

New York, NY

M.S., Bioengineering, KAIST, Seoul, South Korea

B.S., Microbiology, Pusan National University, Pusan, South Korea

Awards and Scholarships

|Korean Women Scholarship |Pusan National University Fellowship |

|Korean Government Scholarship |Best Researcher in Cheil Sugar Co., LTD., Samsung|

Publications

1. Robert H. Scannevin, Sowmya Chollate, Mi-young Jung, Melanie Shackett,

Hiral Patel, Pradeep Bista, Weike Zeng, Sarah Ryan, Masayuki Yamamoto,

Matvey Lukashev, and Kenneth J. Rhodes Fumarate Promote Cytoprotection

of Central Nervous System Cells against Oxidative Stress via the

Nuclear Factor(Erythroid-Derived 2)-Like 2 Pathway (2012). JPET

341:274-284.

2. Jing Wu, Hideaki Kurushima, Hiral Patel, Mi-young Jung, Lenora Volk,

Shoaib Chowdhury, Jason D. Shepherd, Marlin Dehoff, Yueming Li,

Dietmar Kuhl, Richard L. Huganir, Donald L. Price, Robert Scannevin,

Juan C. Troncoso, Philip C. Wong, & Paul F. Worley. Arc/Arg3.1

Regulates an Endosomal Pathway Essential for Activity-Dependent ?-

Amyloid Generation (2011) Cell. 2011 Oct 28; 147(3):615-28.

3. Xin Z, Peng H, Zhang A, Talreja T, Kumaravel G, Xu L, Rohde E, Jung

MY, Shackett MN, Kocisko D, Chollate S, Dunah AW, Snodgrass-Belt PA,

Moore Arnold H, Taveras AG, Rhodes KJ, Scannevin RH. Discovery of 4-

aminomethylphenylacetic acids as ?-secretase modulators via a scaffold

design approach (2011). Bioorg Med Chem Lett.

4. Hairuo Peng, Tina Talreja, Zhili Xin, J. Hernan Cuervo,

Gnanasambandam Kumaravel, Michael J. Humora, Lin Xu, Ellen Rohde,

Lawrence Gan, Mi-young Jung,| Melanie N. Shackett, Sowmya Chollate,|

Anthone W. Dunah, Pamela A. Snodgrass-belt, H. Moore Arnold, Arthur

G. Taveras, Kenneth J. Rhodes, and Robert H. Scannevin . Discovery of

BIIB042, a Potent, Selective, and Orally Bioavailable ?-Secretase

Modulator (2011). ACS Med. Chem. Lett 2, 786-791.

5. Paul H. Weinreb, Dingyi Wen, Fang Qian, Craig P. Wildes, Ellen A.

Garber, Lee Walus, Mi-young Jung, Joy Wang, Jane K. Relton, Joseph

Amatucci, Ruizhong Wang, Frank Porreca, Laura Silvian, Werner Meier,

R. Blake Pepinsky, and Daniel H.S. Lee. Resolution of disulfide

Heterogeneity in Nogo Receptor (NgR1) Fc Fusion Proteins by Molecular

Engineering (2010). Biotechnol. Appl. Biochem 57, 31-45.

6. Jung, M.-Y., Lorenz, L.J., and Richter, J.D. Translational control by

neuroguidin, a eukaryotic initiation factor 4E and CPEB binding

protein (2006). Mol Cell Biol. Jun; 26(11):4277-87.

7. Jung, M.-Y., Huang, Y.-S., Sarkissian, M., and Richter, J.D. Activity-

dependent CPEB phosphorylation and (CaMKII mRNA polyadenylation at

synapses (2002). EMBO, 21(9) 1-10.

8. Irina Groisman, Jung, M.-Y., Sarkissian, M., and Richter, J.D.

Translational control of the embryonic cell cycle (2002). Cell 109,

473-483.

9. Jung, M.-Y., Hof,P. and Schmauss,C. (2000). Targeted disruption of the

dopamine D(2) and D(3) receptor genes leads to different alterations

in the expression of striatal calbindin-D(28k). Neuroscience, 97(3),

495-504.

10. Jung, M.-Y., Scryabin,B.V., Arai,M., Abbondanzo,SA., Fu,D.,

Brosius,J., Robakis,N.K., Polites,H.G., Pintar,J.E., and Schmauss.C.

(1999). Potentiation of the D2 mutant phenotype in mice lacking

dopamine D2 and D3 receptors. Neuroscience, 91(3), 911-924.

11. Jung, M.-Y. and Schmauss,C. (1999). Decreased c-fos responses to

dopamine D1-receptor agonist stimulation in mice deficient for D3

receptors. J. Biol. Chem. 274 (41), 294**-*****.

12. Park, C. G., Jung, M. Y., Choi, Y. W., Winslow, G. M. (1995).

Proteolytic processing is required for viral super antigen activity.

J. Experimental Medicine 181, 1899-1904.

13. Park, H. S., Jung, M.-Y., Oh, M. S., Koh, j. H., Kim, H. S. and Hyun.

H. H. (1987). Isolation and purification of human interferon alpha

from recombinant E.coli by monoclonal antibody column and HPLC gel

filtration. Korean Biochem J. 20(4), 326-334.

14. Kim, K. T., Jung, M.-Y., and Yoo, O.-J. (1985). Purification and

catalytic properties of Stu I restriction endonuclease from

Streptomyces tubercidicus. Korean Industrial Microbiology 25(3), 180-

183.

Presentations

(Slide and Poster)

1. Scannevin RH, Chollate S, Jung MY, Patel H, Bista P, Zeng W, Lukashev

M, Rhodes KJ (2010). Neuroprotective Effects of Dimethyl Fumarate and

Monomethyl Fumarate on Primary Cultures of Human Spinal Cord

Astrocytes After Oxidative Challenge, Biogen Idec, Inc., Weston, 26th

Congress of the European Committee for Treatment and Research in

Multiple Sclerosis.

2. Nastassia Sylvestre, Hiral Patel, Robert Scannevin, Mi-young Jung

(2010). Development of Protocol for Culture of Oligodendrocyte

Precursor Cells and Testing the Effect of FUMARATE, Biogen Idec,

Cambridge, MA.

3. Nastassia Sylvestre, Robert Arduini, Melissa Levesque, Christine Liu,

Stephan Miller, Darren Baker, Robert Scannevin, Mi-Young Jung (2009).

Developing the Binding Assay between Arc and PS1, Biogen Idec,

Cambridge, MA.

4. Jung, M.-Y and Lee, D. (2006). CGN neurite outgrowth assay Biogen

Idec, Cambridge, MA.

5. Jung, M.-Y., Lorenz, L.J., and Richter, J.D. Translational control by

neuroguidin, a eukaryotic initiation factor 4E and CPEB binding

protein (2004). Society for Neuroscience Annual Meeting.

6. Jung, M.-Y., Bernard, D.C., Cao,Q., Lorenz, L.J., and Richter, J.D.

(2002). Vertebrate midline is complexed to the cap binding factor

eIF4E, is in particles in neuronal growth cones, and controls neural

tube formation in embryos. Axon Guidance Annual Meeting, Cold Spring

Harbor Laboratory, NY.

7. Lorenz, L.J., Cao,Q., Jung, M.-Y., Perrimon, N., Van Vactor, D., and

Richter, J.D. (2002). Midline, an eIF4E-binding protein, mediates

longitudinal axon guidance and netrin signaling. Axon Guidance Annual

Meeting, Cold Spring Harbor Laboratory, NY.

8. Jung, M.-Y., Hof, P. R. and Schmauss, C. (1999). Expression of

calbindin-D28K immunoreactivity in the dorsal striatum of mice lacking

dopamine D2, D3, and D2/D3 receptors. Society for Neuroscience Annual

Meeting.

9. Jung, M.-Y. and Schmauss, C. (1998). Altered induction of c-fos

expression following dopamine D1-receptor stimulation in mice lacking

D2, D3, and D2/D3 receptors. Society for Neuroscience Annual Meeting.

10. Jung, M.-Y., Skryabin, B. V., Arai, M., Brosius, J., Pintar. J. E. and

Schmauss, C. (1997). Increased expression of dopamine D3 receptors in

brains of D2-receptor-deficient mice. 50th Anniversary Scientific

Symposium, New York, NY.

11. Jung, M.-Y., Skryabin, B. V., Arai, M., Brosius, J., Pintar. J. E. and

Schmauss, C. (1997). Increased expression of dopamine D3 receptors in

brains of D2- receptor-deficient mice. Society for Neuroscience Annual

Meeting.

12. Chung, K. T., Jung, M.-Y., Yoo, W. I., and You, K.-S. (1989) An FPLC

procedure for purification of hepatitis B surface antigen produced in

the culture of recombinant mammalian Cell. 5th FAOB Congress.

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