Project Research
Resume:
Michael J. DiBiasio-White
*******.**************@*****.***
Cell 508-***-****
Home Address
somerset, MA 02726
EDUCATION
University of Massachusetts Dartmouth North Dartmouth, MA
B.S. in Biology Graduated, May 2010
HONORS AND AWARDS
Hoff Scholar( Hoff scholarship) sponsored by Umass Dartmouth Alumni
Honorary Mention for Oral presentation at BioNess conference- 2009
Deans List-2007-2010
First place in poster presentation at BioNess conference – 2009
Research and Related Jobs
Constellation pharmaceuticals Cambridge, Ma
Biology research (contractor) 2011 May-present
o Work for a biopharmaceutical company; supporting production and purification
of recombinant and WT proteins.
o Protein purification (insect cell and bacteria)
o Protein refolding and reconstitution
o Purification of His and Flag tag proteins using standard chromatographic techniques
o Some experience in Size exclusion chromatography (HPLC, Akta system)
o Protein expression/ solubility optimization and protocol optimization
o Buffer and media preparation
o Bacterial transformations and sequencing
o Protein quantification (UV absorbance, BCA)
o Western blotting, SDS PAGE
Boston Open Labs Fall River, MA
Intern May- August 2010
Developed protein and amino acid catalogue
Ordered supplies and equipment
Managed phone calls and customer calls
Phosphorex
Intern May- August 2009
Research project focused on: Biomass conversion to Biofuels
Developed new pre-treatment method for biomass conversion to glucose by sonication without chemical use
Grew and extracted enzymes from fungus using several methods
Used enzymes and made buffers
University of Massachusetts Dartmouth North Dartmouth, MA
Independent Researcher September 2008- May 2010
Worked in the molecular biology lab
Analysis of Wnt clade (1,3,6,9,10) in oro-facial clefting
Used techniques including: PCR, DNA/RNA isolation gel electrophoreisis and in situ hybridzation to study morpholino knockouts of Wnt clade affect on development
Researcher September-December 2008
Worked on isolating Bacterial DNA and transformation
Pond water DNA isolation of bacteria present
PCR, DNA/ RNA isolation techniques used
LAB TECHNIQUES
Cloning and subcloning, DNA sequencing, vector and plasmid preparation, staining and microscopy, DNA and RNA extraction, gel extraction, bacterial transformation using restriction enzymes, gene cloning, restriction mapping, gel electrophoresis, SDS-Page, Western blot, PCR, Protein expression and purification, media prep, autoclave, HPLC ( size exclusion chromatography)
INSTRUMENT USE
JSM 5610 Scanning Electron Microscopy (SEM)
Hach DR-4000 UV/Vis Spectrophotometer
Olympus BH-2 Optical Microscope
PCR and cloning techniques
SKILLS
Excellent verbal, written communication, and organization skills. Have frequently given presentations to large audiences.
Extensive use of Microsoft Excel to present data in tables and graphs format
Knowledgeable/ past experience with research paper writing with publication format
Ability to operate with efficacy SEM to achieve images.
Efficiency in cloning and subcloning
ACTIVITIES
Secretary of Pre-Health Club (04/2009-05/2010)
Active member of Pre-Med club (09/2006-present)
Volunteer at molecular biology lab in Umass Dartmouth (10/200*-**-****)
Volunteer after hours tutor/ homework help at Brown School, Swansea, Ma ( 3 years)
Participant in BioNes scientific conference (2009)
PUBLICATIONS/ PRESENTATIONS
2009- BioNess Conference. Poster Presentation: “Analysis of Wnt Clade in Oral Facial Clefting.”
Lane H. Wilson, Michael J. Dibiasio-White, Tracie Ferriera.
References available upon request:
Email: *******.**************@*****.***
Cell: 508-***-****
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