Research Technician
Resume:
Megan M. Bucher
*****.******@*****.***
Northumberland, PA 17857
Objective: Obtain a full time position as a laboratory technician to further my experience in research and development
Education:
Wilkes University Wilkes-Barre, PA
Bachelor of Science in Biology May 2009
Minor in Chemistry
GPA: 3.52/4.00
Dean’s List: Fall 2005, Fall 2007, Spring 2008, Fall 2008, Spring 2009
Senior Research:
Project Leader: Localization of TGF-B in Wild-Type and TSP-KO mice, Dr. Valerie Kalter
Work Experience:
Geisinger Health Services, Weis Center for Research
Genomics Core Research Technician August 2009-Present
•Lead data collection for gene mutation study of Polycythemia Vera by isolating DNA, amplifying via PCR, and organizing results
•Managed several DNA sequencing projects including Non-Alcoholic Steatohepatitis by collecting specimens, updating data, and storing samples
•Coordinated data entry for research projects using Microsoft Excel and Freezerworks applications
•Isolated DNA by using Qiagen robots M48 and Symphony Models
•Collaborated with scientists and staff to create future projects in partnership with research institutions
Wilkes University Biology Department
Intern/Research Assistant, Dr. Valerie Kalter Summer 2008
•Cultured lung fibroblasts both from wild-type and thrombospondin deficient mice
•Prepared histological slides of mouse brains in order to view anatomical differences between wild-type and thrombospondin deficient mice
•Updated lab mice conditions by changing cages, filling water and food, and monitoring activity
•Counted and recorded bacteria colonies from mouse colon on MacConkey agar
•Aided in RNA purification from mouse colon
Activities:
Sigma Alpha Pi, The National Leadership Honor Society 2008 - 2009
Beta Beta Beta, The Biological Honors Society, Historian 2007 - 2009
Wilkes University Women’s Tennis Team 2005 - 2008
Pre-Med Day Team, High School Program Volunteer 2007, 2008
Laboratory Techniques & Skills:
DNA Isolation
PCR
Immunohistochemistry
RNA Purification
Cell Culturing
Microscope Slide Preparation
Genotyping
Spectrophotometry
Data Management
Laboratory Machinery:
Qiagen M48
Qiagen Symphony
Nanodrop ND-1000 Spectrophotometer
Applied Biosystems Real-Time PCR 7500
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