JR. Scientist

David Vagratian

Vilnius, Lithuania • 370-*-***-**** • **********@*****.*** • US Citizen

Summary of Qualifications:

• Extensive hands on experience with RT-qPCR and qPCR.

• Experience with basic molecular biology techniques.

• Experience maintaining an accurate and up-to-date laboratory notebook.

• Experience recording data and reports according to established SOPs.

• Ability to handle multiple assignments under pressure and meet deadlines.

• Proficient in Microsoft Word, Excel, PowerPoint and GIMP.

• Excellent written, oral and interpersonal communication skills.

Work Experience:

UAB Fermentas, Vilnius, Lithuania, March 2009 – Present

Jr. Scientist, Research and Development

• Developed RNase test for product purity testing through Denaturing Urea-PAGE using radiolabeled nucleotides.

• Improved electrophoresis RNase tests for current and future products.

• Responsible for validating Taq DNA polymerase and qPCR master mix product purity and quality according to ISO standards and established SOPs.

• Assisted One-Step and Two-Step RT-qPCR product development teams with buffer optimization and RNA integrity improvement.

• Investigated mutant Reverse Transcriptase interactions with substrates and Taq DNA polymerase during One-Step RT-qPCR.

Education:

Bachelor of Arts in Cell/Molecular Biology, May 2008

Washington & Jefferson College, Washington, Pennsylvania

Experience:

Independent Study, September 2007- March 2008

“Development of Innervation in the Ventral Diaphragm Muscle in Manduca sexta”

• Performed multiple dissections on Manduca sexta pupae and adults.

• Performed tissue preparations, pharmacological manipulations, and histological techniques.

• Visualized synaptotagmin immunoreactivity through a laser scanning confocal microscope.

• Delivered presentation at the 29th Annual Undergraduate Biology Symposium for Western Pennsylvania.

Lab Assistant: Organic Chemistry and General Biology, September 2006– May 2007

• Prepared chemical reagents, maintained laboratory equipment, and mentored students.

Class Projects, 2005

“Indirect Immunofluorescence Detection of GM130 in Golgi apparatus in HeLa Cells”

“Benzaldehyde Ineffectiveness at Inducing Apoptosis in HeLa Cells”

• Presented findings at poster presentations.

Technical Skills:

• Extensive hands on experience with radiolabeling nucleotides, autoradiography, PAGE (conventional and DCode BIO-RAD Universal Mutation Detection System), SDS-PAGE, Western / Southern / Northern blots, ELISA, agarose gel electrophoresis, protein-gel shift, Bradford protein assay, Agilent 2100 Bioanalyzer, NanoDrop, UV/VIS spectroscopy, scintillation analysis endpoint, RT-qPCR and qPCR (ABI 7000, 7500 Fast, StepOne Plus and BioRad iCycler iQ), pharmacological manipulations, immunohistology, confocal lazer scanning microscopy, cell culture (HeLa), bacterial cultures, DNA preps, RNA and protein extractions, transfection, affinity and agarose columns, titration, and insect dissections.

Contact this candidate