Project Quality Control

AABHA CHORDIA

Email: ********@*****.***

Mobile: 857-***-****

SUMMARY OF QUALIFICATIONS

Performed HPLC and TLC for synthetic compound characterization and formulation analysis at the Center for Pharmaceutical Biotechnology and Nanomedicine (Director: Dr. Vladimir Torchilin) at Northeastern University

Adept at performing mammalian cell culture techniques and cell viability assays

Performed SDS-PAGE, ELISA and Western blot immunoassays for isolation and quantification of proteins

Expertise in operating instruments for the biophysical characterization of delivery systems

Assisted in carrying out invivo biodistribution studies of a novel delivery system for gene therapy developed for tumor targeting and establishment of tumor bearing mice model

EDUCATION

Northeastern University, Boston, MA (April 2012)

Master of Science in Biomedical Sciences- Pharmaceutics and Drug Delivery Systems

Relevant Coursework: Biochemistry, Anatomy, Physiology and Pathophysiology, Molecular Cell Biology, Advanced Drug Delivery Systems, Pharmaceutical Sciences Laboratory, Experimental Design and Biostatistics, Ethics in Biological Research, Introduction to Industry Research Guidelines, Pharmacokinetics and Drug Metabolism, Advanced Physical Pharmacy

University of Mumbai, Mumbai, India (April 2010)

Dr. Bhanuben Nanavati College of Pharmacy

Bachelor of Pharmacy

RESEARCH SKILLS

Centre for Pharmaceutical, Biotechnology and Nanomedicine, Northeastern University, Boston, MA

Research Assistant (March 2011-Present)

Project#1: Preparation and evaluation of transferrin-conjugated polymeric/lipidic drug/gene delivery system

Thin Layer Chromatography

Purification of polymers by dialysis

ELISA for immunodetection and quantification of transferrin

BCA protein assay, TNBS assay for amine quantification

Size and Zeta potential measurement of the delivery system

Agarose gel electrophoresis to evaluate the condensation capacity of the delivery system

HPLC for determination of encapsulated paclitaxel in the delivery system

Project#2: P-glycoprotein (P-gp) downregulation in mammalian tumor cells using transferrin conjugated carrier

Loading the delivery system with siRNA targeting the MDR1 gene that codes for P-gp

SDS-PAGE for separating mammalian cell culture proteins and isolating P-gp

Western blot to evaluate P-gp downregulation

Project#3: Evaluation of intracellular uptake of the drug/gene delivery system and cell viability after treatment.

Establishment, maintenance and preservation of mammalian tumor cell lines (human and murine)

Cell titer blue assays to evaluate drug and polymer cytotoxicity

Flow cytometry for evaluation of P-gp silencing efficacy

UV and fluorescent spectrophotometry

Confocal and fluorescent microscopy to check the intracellular uptake of the delivery system

Project#4: Biodistribution of Fluorescein-labeled siRNA complexed in a carrier made of Phospholipid-modified PEI and PEG-PE

Establishment of subcutaneous 4T1 tumor-bearing BALB/c mice model

Intraperitoneal, intravenous, subcutaneous injection procedures

Anesthesia (intraperitoneal:ketamine/xylazine, isoflurane; euthanasia procedures: carbon dioxide asphyxiation and cervical dislocation)

Mouse organ harvesting and tissue homogenization

Miscellaneous Lab skills acquired via Pharmaceutical Sciences Laboratory course:

Enzyme kinetics experiments, formulation of gold and gelatin nanoparticles, affinity chromatography, PCR, Transfection and Restriction enzyme digests, Nanodrop® for protein and RNA quantification

ACADEMIC PROJECTS, PUBLICATIONS and AWARDS:

Publication:

V. Suvarna, S.Shetye, A. Chordia, “Bio Ceramics” Journal of Pharmacy Research- Volume-2 Issue-10, 2009.

Poster Presentation:

Essex,S., Navarro, G.,Chordia, A. and Torchilin, V.P. “Novel Polyethylenimine Based Nanocarrier Improves Systemic Tumoral Delivery of siRNA” poster presented at 2012 Annual Meeting of American Association of Pharmaceutical Scientists-NERDG. (April 20th, 2012)

Essex,S., Navarro, G.,Chordia,A. and Torchilin, V.P. “Novel Polyethylenimine Based Nanocarrier Improves Systemic Tumoral Delivery of siRNA” poster presented at RISE 2012: Research, Innovation and Scholarship Expo at Northeastern University, Boston (March 29, 2012)

Poster abstract S. Essex, G. Navarro, A.Chordia and V.Torchilin “Mixed Carrier Made of Phospholipid-modified PEI and PEG-PE Improves Systemic Delivery of siRNA in tumors” accepted at the 39th Annual meeting and exposition of the Controlled release society (CRS 2012) to be held at Quebec, Canada in July 2012

A.Chordia, N.Dave, V.Suvarna, S.Patil “Anti Fungal Activity of Selected Plant Extract on Human Fungal Pathogen” poster presented at the 61st Indian Pharmaceutical Congress (IPC), Ahmedabad, India (December 2009)

INDUSTRIAL TRAINING and VISITS

Summer training at Renumed Pharmaceutical Lab (May 2009-June 2009)

Assisted in the formulation and development of ibuprofen, paracetamol, sildenafil, sumatriptan succinate tablets

Worked on lab scale and pilot scale equipments such as multi-station tablet press, manual tablet punching machine, spheronizer, oscillator-granulator, fluidized bed system and standard coating pans

Performed Quality control tests like:

HPLC and TLC to detect impurities in the sumatriptan succinate tablets

Disintegration testing

Dissolution testing

SKILLS

MS Office Suite 2007

Working knowledge of research data analytical tools like Graph Pad Prism 5.04, ImageJ

Proficient at using useful research tools like PubMed and Endnote

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