Assistant Technician

Lauren Meldi Sholl

***************@*.************.***

720A Hinman Ave Apt 3N, Evanston, IL 60202, 417-***-****

Educational Information

Northwestern University, Evanston, IL, USA 2008-present

• Master’s degree in Molecular Biosciences, GPA: 3.67

University of Missouri-Columbia, Columbia, MO, USA 2002-2006

• B.S., Biological Sciences; Minor: Spanish; GPA 3.54

• Dean’s list (2002-2006)

Research Experience

Northwestern University, Evanston, IL, USA 2008-present

Graduate Student with Jason Brickner, Ph.D.

The molecular mechanism of gene localization in the nucleus of S. cerevisiae

• Use confocal microscopy with immunofluorescence to determine gene localization with respect to the nuclear periphery

• Identify the minimal DNA element that is responsible for targeting the active HSP104 gene to the nuclear periphery

• Determine the mechanism of gene targeting through EMSA, Co-immunoprecipitation, and chromatin immunoprecipitation experiments

University of Kansas Medical Center, Kansas City, KS, USA

Research Associate with Robert De Lisle, Ph.D. 2006-2008

The role and pathophysiology of cystic fibrosis in the cftr knockout mouse small intestine.

• Used mass spectrometry and qRT-PCR to find gene expression differences between wild type and cftr knock-out mice

• Determined eicosanoid differences between wild type and cftr mice

• Performed small intestine in vivo and in vitro motility assays

• Determined affect of drugs on small intestinal motility

• Determined the development and onset of CF symptoms including small intestinal bacterial overgrowth, motility, and eicosanoid imbalances

University of Missouri Columbia Hospital, Columbia, MO, USA Summer 2006

Laboratory Technician

• Received patient bacteria samples and streak on appropriate media for detection of bacteria

• Used microscopy and staining to determine if gram negative or gram positive bacteria are present

• Performed stat culture analysis

University of Missouri Columbia, Columbia, MO, USA 2002-2006

Summer undergraduate research student for Habib Zaghouani

The development of myelin reactive T-cells in multiple sclerosis

• Determined severity of multiple sclerosis phenotype in mice receiving drugs

• Cell culture

• Assisted graduate student with ELISAs

Teaching Experience Northwestern University, Evanston, IL, USA

• Teaching Assistant, Genetics and Evolution laboratory, Fall 2009

• Teaching Assistant, Biochemistry, Fall 2010

Skills and Techniques

• Confocal and spinning disk fluorescent microscopy

• Genetic manipulations in yeast

• PCR and cloning

• Chromosome conformation capture

• Protein extraction and immunoprecipitation experiments

• Chromatin immunoprecipitation experiments followed by qRT-PCR

• RNA extraction

• Genotyping and maintaining transgenic mouse line

• Enzyme immunoassay

Publications

Brickner, D.; Ahmed, S.; Meldi, L. et al. Transcription Factor Binding to a DNA Zip Code Controls Interchromosomal Clustering at the Nuclear Periphery. 2012. Developmental Cell.

De Lisle, R.C., Meldi L, and Mueller R. Intestinal smooth muscle dysfunction develops postnatally in cystic fibrosis mice. J Pediatr Gastroenterol Nutr. 2012. Jun 13.

Meldi, L. and J.H. Brickner, Compartmentalization of the nucleus. Trends Cell Biol, 2011. 21(12): p. 701-8.

De Lisle, R.C., R. Sewell, and L. Meldi, Enteric circular muscle dysfunction in the cystic fibrosis mouse small intestine. Neurogastroenterol Motil, 2010. 22(3): p. 341-e87.

De Lisle, R.C., et al., Mast cells and gastrointestinal dysmotility in the cystic fibrosis mouse. PLoS One, 2009. 4(1): p. e4283.

De Lisle, R.C., et al., Altered eicosanoid metabolism in the cystic fibrosis mouse small intestine. J Pediatr Gastroenterol Nutr, 2008. 47(4): p. 406-16.

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