Engineering Supervisor
Resume:
EDUCATION:
Postdoc. (Supervisor: Prof. Janet Moradian-Oldak), Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA 90033, USA
2008.3-2010.3
Postdoc. (Supervisor: Prof. George H. Nancollas), Chemistry Department Sciences Complex, The State University of New York, NY 14226, USA
2003.9-2007.12
Ph.D. (Supervisor: Prof. Ji-Yong Chen), Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, China
1999.9-2002.7
MSc. (Supervisor: Prof. Jiu-Li Luo), Department of Chemistry, Sichuan University, Chengdu 610064, China
1995.9-1999.7
BSc. Department of Chemistry, Sichuan University, Chengdu 610064, China
RESEARCH EXPERIENCE:
During postdoc period, the projects: Enamel Proteins (amelogenin, enamelin, KLK4 and MMP20) Structure and Their Cooperation and Functions on Enamel Growth and Maturation. (1) Determine Kinetics of Calcium Phosphate (CaP) Nucleation & Mechanisms of CaP Phase Transformations by amelogenins. Preliminary results show the assumption that proteins and their cooperative combinations, peptides and inorganic ions, such as magnesium all exhibit roughly equivalent biological control over crystal nucleation and growth during mineralization, either by localizing crystallization or by stabilizing otherwise short-lived amorphous forms of calcium phosphate. (2) In addition to the spherical particles associate, full length of amelogenin either tend to form an anisometric assembly associate which can go on to assemble into chains-like formation. At the certain condition, amelogenin monomer and oligomers nanoparticles tested within unstructured state. During amelogenin (rP172) proteolysis by rpMMP-20, the nanorods structure of co-assembly between full- length rp172 and their cleavage products were investigated by TEM technique. Head to Tail amelogenin monomers connected into oligomers, these oligomers stacked into nanorods. As the building blocks, this nanorod structure was proposed forming the enamel matrix in vivo. In vitro investigation of amelogenins cooperation with enamelin enamelin has the tendency of cooperation with amelogenin into oligomeric particles lead dispersion of amelogenins aggregation, at the same time, the aspect ratio of OCP crystals was markedly increased in a dose dependent manner.
HR-TEM, RP-HPLC, CD, Flourescence, dynamic light scattering (DLS), SEM, FTIR, X-ray have been used to examine proteins structures and assembly morphologies evolution and the crystals nucleation and growth. Especially for HR-TEM, I have almost 6 years experience of using it for testing morphology and quantity of protein structure and crystal lattice. At the same time, CC/Dual CC techniques have be used to quantitatively examine biomineralization process in vitro. Induction times for homo/heterogeneous nucleation, particle evolution of nuclei with time, and subsequent growth rate for hydroxyapatite (HAP) have been investigated.
During my studies of my Ph.D. thesis, my projects are 1) Modify surface of surgical titanium implants with CaP ceramics and collagen so that they can be bioactive; 2) perform electrochemical deposition experiments, correlative instrument examinations, cell culture and animals bones implanting were used to test in vivo bioactivity of the modified titanium surface. It found that bioactive calcium phosphates coating and collagen nanofilm can be formed on the titanium implants surface, resulting improved binding efficiencies between implants and the bone. I have good experience in cells culture and small animal surgery ( bone repairing or subcutaneous implantation surgery on rats, rabbits and dogs), western blotting, HE staining, ELISA, RT-PCR; immunochemistry; Facs; Elisa; immunoflourescence staining and Co-Immunoprecipitation.
I am a self-motivated, and independent researcher, can collaborate very well with the colleagues. Analyzing and solving problems on my own account is wonderful. I have good oral and written communication skills in English.
My research interests focus on multi-disciplinary fields: Proteins Structure and Function (include crystallography, nanoscale composites, molecular self-assembly, superamolecular protein-protein, protein template nanostructures, mechanism of mineralization bone, dentin, and enamel, and calcium protein signaling investigation. Otherwise, proteins (peptides) medicine, surface nanotechnology, biomaterials, materials interface chemistry are my expanding research interesting directions.
FUNDING
1) Principle Investigator, The preparation of the bioactive porous titanium coating on the surface of surgical implant, (The Science and Technology Bureau of Chengdu city, Period: 01/2005-12/2006, RMB: 50,000, N: 05HJSW044).
2) Peptide self-assembled monolayer regulating nucleation and growth of hydroxyapatite. (National Natural Science Foundation of China, RMB: 250,000, N: 21101108/B010404).
PROFESSIONAL ACTIVITY
Member: Chinese Society of Biomaterials
AAAS(Science Program for Excellence in Science)
PATENTS
1.Yang BC, Chen HZ, Yang XD, et al., The Gas of Protecting Device for Porous Titanium Using the Air Plasma equipment, China Patent, Public,( authorization, N:200520035503.9)
2.Yang BC, Chen HZ, Yang XD, et al., The sprayer method design and the Gas of Protecting Device for Porous Titanium Using the Air Plasma equipment, China Patent, Public,( authorization, N: 200510021694.8)
PUBLICATIONS:
1. Xiudong Yang, Zhi Sun, Ruiwen Ma, Daming Fan and Janet Moradian-Oldak. Amelogenin Nanorods Formation During Proteolysis by Enamelysin. J Struct Biol. 176, 220-228, (2011).
2. Xiudong Yang, Daming Fan and Janet Moradian-Oldak. Effect of the 32 kDa Enamelin on Amelogenin Self-assembly. Eur J Oral Sci.119, 351–356, (2011).
3.Daming Fan, Mayumi Iijima, Keith M. Bromley Xiudong Yang, Shibi Mathew and Janet Moradian-Oldak. The Cooperation of Enamelin and Amelogenin in Controlling Octacalcium Phosphate Crystal Morphology. Cells Tissues Organs,194, 2-4, (2011).
4.Xiudong Yang, Baoquan Xie, Lijun Wang, Yueling Qin, Zachary J. Henneman and Gorge H. Nancollas. Influence of magnesium ions and amino acids on the nucleation and growth of hydroxyapatite. CrystEngCom. 13, 1153-1158, (2011).
5.Xiudong Yang, Lijun Wang, Yueling Qin, Zhi Sun, Zachary J. Henneman, Janet Moradian-Oldak, and George H. Nancollas. How amelogenin orchestrates the organization of hierarchical microstructures of enamel. The Journal of Physical Chemistry-B 114: 2293–2300, (2010).
6.Xiudong Yang*, Zhang Bo, Lu Jian, Chen Jiyong, Xingdong Zhang, Zhongwei Gu. Biomimetic Ca-P Coating on Pre-calcified Ti Plates by Electrodeposition Method, Applied Surface Science, 9: 2700-2704, (2010).
7.Xiudong Yang*, Huang Yi, Jiang Bo, et al. Collagen nanofilm immobilized on at surfaces by electrodeposition method, J Biomed Mater Res: Appl Biomater 90B:608-613, (2009).
8.Xiudong Yang, Zhang Bo, Qu Yang Chen Jiyong, Yang Bangcheng, The study of Ca-P coatings prepared by cathode deposition method, Rare Metal Materials and Engineering, 37: 538-541, (2008). (Chinese)
9.Xiudong Yang, Xiong Lu, Qiyi Zhang, et al., BCP coatings on pure titanium plates by CD method, Materials Science and Engineering C 27:781–786, (2007).
10.Xiudong Yang, Teng Lizhao, Lu Jian, Wei Qingrong, Wang Hui, Chen Jiyong ,Yang Bangcheng, Biomimetic Coating on Titanium Metal and Its Excellent Cell Proliferation. Key Engineering Materials 330-332: (2007).
11.Xiudong Yang, Min Tang, Bo Zhang, et al., Calcium Phosphate Coating on AT Titanium Plate by CD and BD methods, Key Engineering Materials 309-311: (2006).
12.Xiudong Yang, Luo Jiuli, Influences of Non-ideality of Externally Controlled Component Multiple Steady States Transition in Regular Solution, Journal of Sichuan University (Natural Science Edition), 3:142-147, (2002). (Chinese)
13. Zhang Qi, Yang Leng, Xiong Lu, Renlong Xin, Yang Xiudong, etc. Bioactive films on metallic surfaces for osteoconduction, J Biomed Mater Res: partA 88:481-490, (2008).
14. Lu xiong, Wang yibo, Yang Xiudong, etc. Spectroscopic analysis of titanium surface functional groups under various surface modification and their behaviors in vitro and in vivo, J Biomed Mater Res: partA 84A: 523–534, (2008).
15. Tang Min, Yang Xiudong, Wu Yao, Cao Yang, Zhou Yu. Biological Evaluation of New Percutaneous Implanting Materials, JOURNAL OF BIOMEDICAL ENGINEERING, 25:143-145, (2008). (Chinese)
16. Renlong xin, Leng Yang, Jiyong Chen, Xiudong Yang et al, Comparison of Biomineralization on Calcium Phosphate Implants in Rabbit and Dog models, Key Engineering Materials. 330-332: (2007).
17.Wei QR, Xiudong Yang, et al, Effect of Inorganic Ions on Morphology and Crystallinity of collagen Fibrils, Oral Presentation at 7th Asia Syposium on Biomedical Materials, Karea, 2006.
18. Afshar A, Yousefpour M, Yang Xiudong, et al., Investigation of morphology and bioactive properties of comsite coating of HA /vinyl acetate on pure titanium, Materials Science and Engineering B, 128:243-249, (2006).
19. M. Yousefpour, A. Afshar, Xiudong Yang, et al., Nano-crystalline growth of electrochemically deposited apatite coating on pure titanium, Journal of Electroanalytical Chemistry 589:96-105, (2006).
20. Lizhao Teng, Hongsong Fan, Xiudong Yang, et al., The Study of New Injectable Root Canal Filling Material, Key Engineering Materials 288-289(2005).
Contact this candidate