Molecular Biologist

VRUSHALEE V. PALSULE

**** *** ******, *** **, Toledo, OH 43614 ● ***************@*****.*** ● 215-***-****

Seeking a full-time opportunity in Research, explore more professional skills in fields in Molecular Biology

SUMMARY

• 3+ years of experience in standard PCR, gel electrophoresis and primer design, BSL-2 biosafety cabinets/hoods

• ~ 1 year of experience in qRT-PCR, probe design, restriction enzyme digestin, ligation, cloning cell culture techniques

• 1+ years of experience in dissection of fish, RNA and DNA extractions, reverse transcription and Agilent bioanalyser

• Comfortable working with computerized instruments and primer design softwares such as oligo and biosearch technology, Adobe Illustrator, Microsoft word, excel and powerpoint (posters and presentations)

• Goal oriented, Effectively accomplish targets before deadline, troubleshooting in team plus independent contributor, maintain lab record

EDUCATION

Master of Science - Cell and Molecular Biology (May ’10)

LOUISIANA TECH UNIVERSITY, Ruston, LA

Thesis Track, Graduate Advisor: Dr. Jeffry Shultz

Course Highlights: Principles of cell and molecular biology, Research methods in biological sciences, Protein engineering, Cancer biology, Virology, Biology seminar, Statistics, Current topics in research

Bachelor of Science – Biotechnology (May ’07)

MUMBAI UNIVERSITY, Mumbai, India

Course Highlights: Cell biology, Genetics, Bichemistry, Biophysics, Biomathematics and Biostatistics, Instrumentation, Microbiology, Immunology, Bio-process technology and Bioinformatics, Molecular biology, DNA Technology and Genetic Engineering, Animal and plant Biotechnology, Tissue culture technology

EXPERIENCE

RESEARCH TECHNICIAN - Lake Erie Research Center, University of Toledo, Toledo, OH (Oct. ’10 – Present)

Project Topic:

• Rapid development of PCR test to detect viral hemorrhagic septicemia (VHS) virus

• Detection of viral particles of VHS virus in experimentally challenged yellow perch

• Development of Real-time PCR for detection of VHS virus

• Comparison between cell culture assay and developed PCR technique to detect molecules of VHS virus present in experimentally challenged and wild caught fish

- Major work: Designing primers and probes, dissection of fish, extraction of RNA from fish tissues, reverse transcription of RNA to cDNA, standardized PCR with internal standard, Agilent Bioanalyser for detection of DNA size and quantity,

qRT-PCR, validation assay experiments, cloning, sequencing form microsatellite DNA

- Assistance in writing grants for projects and publications, Making posters and several presentations

- Maintenance of lab by keeping record of all reagents, ordering, budget handling, acquiring quotes, efficiently storing data on computer as well as in notebook, submission of weekly progress reports

- Conservation genetics as a coursework

TEACHING ASSISTANT - Louisiana Tech University, Ruston, LA (Aug. ’09 – May ’10)

- Lab instruction for four sections of a junior level

- Duties included training students in pipetting, PCR and related laboratory techniques, gel preparation and documentation

TEACHING ASSISTANT - Louisiana Tech University, Ruston, LA (Aug. ’09 – May ’10)

Project Topic: Integration of Multiple Sclerosis Allele Screening and classroom molecular genetics instruction

RESEARCH ASSISTANT - Louisiana Tech University, Ruston, LA (Aug. ’08 – May ’09) Project Topic: Correction of point mutations by Flp and Cre-mediated gene replacement

- To create a recombination vector in pUC19 for correction of gene replacement detecting vectors specific for a particular disease, evolving the corresponding Flp variants.

- Created a Recombinase-mediated cassette exchange (RMCE) with Cre gene

TRAINEE - Thyrocare, Thyroid Hormones Testing Laboratory, Mumbai, India (May ’07)

- Effect of Temperature and Time Duration on Concentration of Triglycerides and HDL

TRAINEE, JASLOK HOSPITAL, Mumbai, India (Apr. ’06)

Department of Assisted Reproduction and Genetics

Training Details: Karyotyping and FISH (Fluorescence in situ Hybridization)

Immunology Department

Training Details: Introduction to ELISA, PCR, Human Lymphocyte Antigen (HLA) matching, Cross Matching, Anti Nuclear Antibodies (ANA), Anti Mitochondrial Antibody (AMA) and Chemiluminescence for HBs, HIV, HCV

TRAINEE, BOMBAY HOSPITAL, Department of Human Genetics, Mumbai, India (Mar. ’06)

- Training in Blood Ammonia, Pyruvate, Gel Electrophoresis, Thin Layer Chromatography, Leucocytes Separation, Lactate and Enzyme Determination, and Alkaptonurea Test

- Performed biochemistry tests on urine samples from various patients to detect genetic deficiencies

SKILL-SET

Tissue Culture Techniques

- Dissection of fish, obtaining organs from fish

- Cell and tissue culture techniques (cancer cell lines and mouse embryonic stem cell culture)

- Transfection of cell culture with fluorescence proteins; mainly GFP

- Efficient handling of Fluorescence microscope for observation of cells

- Cryopreservation and maintaining stocks

- Perform passage of cell culture and maintain plates Viable cell count in haemocytometer

- Plant tissue culture techniques, Mushroom Cultivation

Wet Laboratory Techniques

- Plasmid construction, restriction enzyme digestion, ligation and related techniques used in molecular cloning

- Extraction of RNA from fish tissues, Reverse Transcription of RNA to cDNA

- Chip technology for gel electrophoresis for quantification of molecules present in PCR product

- Sequencing sample preparation and amplification of DNA (PCR), bacterial colony PCR, Gel electrophoresis

- Micro-satellite DNA amplification

- SDS- PAGE, Western blot and Southern blot analysis

- Microbiology techniques such as preparation of agar plates with antibiotics, media/buffer preparation, isolation of pure culture, autoclave, streaking agar plates with efficiency, electroporation

Instruments and Software:

- Frequent use and good knowledge in MEGA, Oligo primer designing, Biosearch technology for probe designing softwares

- Agilent Bioanalyser, Applied Sciences 7500 Fast real time PCR machine, 3130 xl Genetic analyzer for Microsatellite DNA, Any standard PCR machine, NanoDrop 2000, BSL-2 biosafety cabinets/hoods

POSTER:

Louisiana Tech University, Department of Applied and Natural Sciences (Ruston, LA) (April ’12)

The Integration of Multiple Sclerosis Allele Screening and Classroom Molecular Genetics Instruction

Vrushalee Palsule, Dr. Jeffry Shultz, Jamie Toms

PRESENTATIONS

- Viral load of VHSv: Immersion vs. Injection challenged yellow perch at Ohio Fish and Wild life Management Association

Palsule Vrushalee, Pierce Lindsey, Stepien carol (Feb. ’12)

- Progress in rapid PCR testing for VHS at Ohio Department of Agriculture (Sept. ’11)

Pierce Lindsey, Crawford Erin, Willey James, Palsule Vrushalee, Stepien Carol

PUBLICATIONS

- Independent Gene Discovery and testing (Sept. ’10)

Vrushalee Palsule, Dijana Coric, Russell Delancey, Heather Dunham, Caleb Melancon, Denis Thompson, Jamie Toms, Ashley White and Jeffry Shultz

- Detection of fish Viral Hemorrhagic Septicemia Virus (VHSv) using StaRT-PCR: Enhanced quality control with internal standards (In preparation)

Lindsey Pierce, Erin Crawford, Vrushalee Palsule, James Willey, Douglas Leaman, Mohammad Faisal and Carol A. Stepien

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