TD Cutler, Ph.D.

CURRICULUM VITAE

TIMOTHY D. CUTLER

*** ******** ****

Carlisle, Iowa 50047

E-mail: ******.***@*****.***

Phone: 515-***-****

Summary of Qualifications:

Federal Licensure of Animal Biologics; Biological and Chemical in vitro Assay Development with Validation, Optimization and Standardization; Design of Experiments (factorial); Protein Purification and Characterization (WB, PAGE, ELISA, IEP, CIEP); in vivo Model Development; Laboratory and Host Animal Protocol Development; Statistical Analysis; Cell Culture (viral propagation); Bacterial Fermentation; SPC, GLP, GCP, and GMP Experienced; Cross-functional Work Team Leadership / Participation; Publication and SOP Authorship, SOP Departmental Reviewer; Departmental and Laboratory Management.

Education:

Ph.D. Veterinary Microbiology and Preventative Medicine (05-2011) Iowa State University, Ames, IA

Dissertation: Inactivation of PRRSV under static and dynamic conditions using (UV254).

M.S. Microbiology, Minor in Chemistry South Dakota State University, Brookings, SD

Thesis: Superantigenic effects of Staphylococcal Enterotoxins Type A and Type B on peripheral blood lymphocytes of meat producing animals.

B.S. (Double major) Microbiology and Biology South Dakota State University, Brookings, SD

Professional and Research Experience:

Midland Bioproducts Corporation, 2011 to 2012:

Research and Development Department Manager: Managed the R and D Department

• Supervised 5 FTEs, conducted budgeting, project prioritization

• Managed the support of LC matrices for the production of commercial antisera

• Managed (and conducted) the purification of proteins from sera

o ATIII and Beta2 microglobulin preparative chromatography (FPLC)

o Protein characterization (PAGE, IEP and CIEP)

BioProtection Systems, ISU Research Park, Jan to July 2011:

Post Doctoral Fellowship: Vaccinology (aGal technology)

• VLP production (shaker and wavebag technology)

• Recombinant protein purification/characterization

• in vitro assay development (WB, Dot Blot, PAGE)

• Cell culture (CHO, Sf-9, Sf-21)

Iowa State University, 2005 to 2010:

Research Assistant: Veterinary Diagnostic and Production Animal Medicine

• Developed techniques for the inactivation and quantification of PRRSV

• Investigated use of UVC radiation to inactivate viral pathogens

Diamond Animal Health, Des Moines, IA. 1998-2002:

Research & Development Scientist: Managed the Immunochemistry Laboratory

• ELISA development, optimization, standardization and transfer of techniques and assays to B. Q. C. for BVD 1 and 2, Bartonella henselae, Infectious Pancreatic Necrosis Virus, K99 E. coli, BCV, BRV, H. somnus, Pasterella multocida

• Research reports, Regulatory submissions and SOP writing

• Vaccine development for intranasal Feline Trivalent Vaccine including production of pre-licensing serials, animal work, virus titration

• Equine influenza product egg production optimization

• Protein extraction, purification, characterization and concentration

• Supervised 2 FTEs for the development and standardization of ELISAs

Good Samaritan Health Systems, Kearney, NE. 1996 to 1998:

Outcomes Coordinator/Statistician: Served as the Health Systems Statistical Analyst

• Performed statistical analysis, developed and maintained databases

• Management and implementation of the CHI Patient Satisfaction Program

• Co-managed and co-implemented the Clinical Outcomes Program

• Presented Quality Management Programs and Outcome/Results Programs to

physicians, administration, employees, and the public

SmithKline Beecham / Pfizer Animal Health, Lincoln, NE. 1993-1996:

Biological Quality Control Technologist: In vitro assay development

• Determined the sensitivity, reproducibility, reagent validation for in vitro assays

• Specific assays: Lyme Disease (NS1 OspA), Bovine and Porcine Trypsin, Sodium Thiosulfate, Erysipelothrix rhusiopathiae, Tetanus Toxoid, FELV

• Defended results and interpretations under corporate and regulatory review

• Served on project teams and chaired various sub-teams including: Erysipelothrix rhusiopathiae, Pasteurella hemolytica, Tetanus Toxoid, Borrellia burgdorferi (NS1 OspA), FELV

• SOP writing, corporate reports and USDA and EEC submission preparation

• Served on facility and departmental GLP and SOP working committees

Grand Laboratories, Larchwood, IA 1987-1990:

R and D Project Manager: Successful federal licensure of animal biologics (bacterins)

• Development of timelines, clinical study protocols, regulatory submissions

• Developed experimental bacterins, conducted safety and toxicity studies

• Planned then supervised host and lab animal immunogenicity studies

• Evaluated, interpreted, and significance tested laboratory and clinical trial data

• Developed quantitative and qualitative in vivo models and in vitro tests

• Supervised 2FTEs and indirect reports (laboratory and animal care technicians)

Awards:

Graduate Scholar Program, (Kansas State University), 2009: ($23,999 scholarship)

Gamma Sigma Delta Honor Society 2006: May induction

Instructor of the Quarter 2005: Hamilton College (now Kaplan University)

Recognized Mentor 2004: ISU Student Scholars and Leaders

SmithKline Beecham 1994: Picture Award

Grand Labs 1989: The Coveted Grand Laboratories Donut Award

Professional Memberships:

Sigma Xi Research Society

American Association of Clinical Chemists

Refereed Publications:

1) Kinetics of UV254 inactivation of selected viral animal pathogens in a static system. Cutler T.D., Wang C., Qin Q., Hoff S.J., Zhou F., Warren K.J., Ridpath J.F., Yoon K-J., Zimmerman J.J. 2011, Journal of Applied Microbiology, 110.

2) Median infectious dose (ID50) of porcine reproductive and respiratory syndrome virus via aerosol exposure. Cutler T.D., Kittawornrat A., Hoff S.J., Wang C., Zimmerman J.J. 2011, Veterinary Microbiology, 151 (3-4).

3) Review of ultraviolet and the mechanisms underlying the inactivation of infectious agents by ultraviolet irradiation. Cutler T.D., Zimmerman J.J. 2011, Animal Research Reviews 11 (3).

4) Effect of temperature and relative humidity on ultraviolet inactivation (UV254) inactivation of airborne porcine respiratory and reproductive syndrome virus. Cutler T.D., Wang C., Hoff S.J, Zimmerman J.J. 2012, Veterinary Microbiology 107 (1-2).

5) A method to quantify infectious airborne pathogens at concentrations below the threshold of quantification by culture. Cutler T.D., Wang C., Kittawornrat A., Hoff S.J., Zimmerman J.J. 2012, Canadian Journal of Veterinary Research (in press).

6) Mitogenic Effects of Staphylococcal Enterotoxins on Bovine and Porcine Peripheral Blood Lymphocytes. Cutler T.D., and Hurley, C.J., 1995. South Dakota State University Thesis.

Non-refereed

Good Samaritan Health Systems newsletter

Inactivation of PRRSV with the use of TiO Photocatalytic System. Genesis Air Incorporated, Lubbock, TX.

Oral Presentations:

1) Cutler T.D., Wang C., Qin Q., Hoff S.J., Zhou F, Warren K.J., Miller C., Ridpath J.F., Yoon K-J., Zimmerman J.J. December 5, 2009. Median infectious dose (ID50) of PRRSV isolate MN-184 for young pigs via aerosol exposure. 2009 International PRRSV Symposium. Chicago, Illinois.

2) Cutler TD, Hoff S, Wang C, Warren K, Zhou F, Qin Q, Miller C, Ridpath J, Yoon K-J, Zimmerman J. October 10, 2009. UV254 Inactivation of Selected Viral Pathogens. 52st Annual Conference, American Association of Veterinary Laboratory Diagnosticians. San Diego, California.

3) Cutler TD, Hoff SJ, Wang C, Warren KJ, Zhou F, Qin Q, Miller C, Ridpath JF, Yoon K-J, Zimmerman J. March 7, 2010. UV254 inactivation of selected viral pathogens. Annual meeting, American Association of Swine Veterinarians. Omaha, Nebraska.

4) Cutler TD, Hoff SJ, Wang C, Warren KJ, Zhou F, Qin Q, Miller C, Ridpath JF, Yoon K-J, Zimmerman J. December 7, 2009. UV254 inactivation of selected viral pathogens. 90th Ann Conference of Research Workers in Animal Diseases. Chicago, Illinois.

5) Cutler TD, Kittawornrat A, Hoff S, Wang C, Zimmerman J. December 7, 2009. Median infectious dose (ID50) of PRRSV isolate MN-184 for young pigs via aerosol exposure. 90th Ann Meet Conference of Research Workers in Animal Diseases. Chicago, Illinois.

6) Cutler TD, Kittawornrat A, Hoff S, Wang C, Zimmerman J. December 4, 2009. Median infectious dose (ID50) of PRRSV isolate MN-184 for young pigs via aerosol exposure. 2009 International PRRSV Symposium. Chicago, Illinois.

Poster Presentations:

1) Cutler TD, Kittawornrat A, Hoff S, Wang C, Zimmerman J. March 7, 2010. Median infectious dose (ID50) of PRRSV isolate MN-184 for young pigs via aerosol exposure. Annual meeting, American Association of Swine Veterinarians. Omaha, Nebraska.

2) Cutler TD, Hoff SJ, Wang C, Warren KJ, Zhou F, Qin Q, Miller C, Ridpath JF, Yoon K-J, Zimmerman J. December 5, 2009. UV254 inactivation of selected viral pathogens. 2009 International PRRSV Symposium. Chicago, Illinois.

Teaching and other experience:

Teaching Assistant, Iowa State University, 2003 to 2006:

• Instructed Comparative Biology Laboratory

• Instructed Anatomy and Physiology Laboratory

Adjunct Lecturer, Hamilton College, 2003 to 2005

• Instructed Anatomy and Physiology 1

The Cutler Group (L.L.C.), Carlisle, IA. 2001 to 2003

• Real Estate Investment and Development Company

• Income Tax Specialist H & R Block

Teaching & Research Assistantship S. Dak. State University 1990 to 1993:

• Instructed Analytical Chemistry I and II, Biochemistry I and Biology I Laboratory

• Researched the Superantigenic effects of Staph. Enterotoxin Type A & Type B on Peripheral Blood Lymphocytes

Grants awarded:

1) Infectious Dose for PRRSV MN-184: National Pork Board $45,366

2) Effect of Temperature and Relative Humidity on Ultraviolet Inactivation of Influenza Type A and BVDV: National Pork Board $39,839

3) Effect of Temperature and Relative Humidity on Ultraviolet Inactivation of PRRSV MN-184: IA Attorney General’s Office $43,046

4) Graduate Scholar Program: Kansas State University $23,999

5) State of Iowa Capital Asset Award $500,000

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