Medical Project

CURRICULUM VITAE

Byung O. Lee

**** ********** **.

San Marcos, CA 92078

Tel) 760-***-****

Email) ********@*****.***

EDUCATIONAL BACKGROUND

Mar. 1986 - Feb.1992 B.A Dept. of Microbiological engineering, Kon-kuk

University, Seoul, Korea (This period includes 2 years of military

service)

Mar. 1992 - Feb.1994 M.A Dept. of Microbiological engineering, Kon-kuk

University, Seoul, Korea

Thesis: Gene cloning and the production of Biosurfactant from Bacillus sp.

Oct. 1993 - Feb. 1994 Research Student in Osaka University Medical School

Mar. 1994 - Feb. 1998 Ph.D. Molecular Oncology Lab. Osaka University

Medical School, Suita-city, Osaka, Japan

Thesis: Elevated levels of the soluble form of bone marrow stromal cell

antigen 1(BST-1) in the sera of patients with severe Rheumatoid Arthritis

PROFESSIONAL EXPERIENCE

Oct. 1993 - Feb. 1994 Research Student in Osaka University Medical

School

Description: This course is a pre-Ph.D. program. This program trains Ph.D.

student candidates for proper scientific orientation, including the

scientific method.

Feb. 1994 - Feb. 1998 Ph.D. program in Osaka University Medical School

Description: During this period, I was involved in clarifying the role of

a new molecule. This molecule is related to the disease kinetics of

Rheumatoid Arthritis. I found this molecule is upregulated in severe

Rheumatoid Arthritis patients. I worked closely with my mentor and a

technician.

Feb. 1995 - Feb. 1997 Teaching Assistant in Osaka University Medical

School

Description: Performed lectures to the students of the Medical school for

Immunology and Cell Biology.

Apr. 1998 - Mar. 2003 Postdoctoral fellowship, Trudeau Institute,

Saranac Lake, NY

Description: Major duty of this job was designing and clarifying the role

of CD40, including making decisions on the direction of research.

Publication and presentation in scientific conferences was also a major

activity.

Apr. 2003 - Mar. 2005 Research Scientist, Trudeau Institute, Saranac

Lake, NY

Description: Designed the majority of research project, and distributed

assignments to other scientific staff members. Engaged in collaborations

with other scientists at different institutions. Publication and

presentation in scientific conferences was also a major activity.

Apr. 2005 - Mar. 2012 Assistant member, Vaccine Research Institute, San

Diego, CA

Description: Manage a laboratory as a principal Investigator. Write

government research grants mainly for NIH research programs. Design own

research project, and distribute assignments to other scientific staff

members. Engage in collaborations with other scientists at different

institutions. Publication and presentation in scientific conference is

crucially included.

April. 2012 - Present Adjunct faculty, Vaccine Research Institute, San

Diego, CA

Description: Manage a laboratory as a principal Investigator as part time.

Write government research grants mainly for NIH research programs. Design

own research project, and distribute assignments to other scientific staff

members. Engage in collaborations with other scientists at different

institutions. Publication and presentation in scientific conference is

crucially included.

SCIENTIFIC MEMBERSHIP

1998 - present Member of the American Associates of Immunologists

2002 - presen Sigma Xi The Scientific Research Society

1 AVAILABLE RESEARCH TECHNIQUES

Animal experiment (19 years of experiences)

- surgery

- bone marrow transplantation

- thymectomy, splenectomy, survival surgery

- intravenous, intranasal, intratracheal, intradermal, subcutaneous

injection

- irradiation

- making rabbit polyclonal antibody

- making mouse monoclonal antibody

Cell Biology

- cell line establishment

- in vitro cell activation

- cell fractionation

- in vitro dendritic cell generation

- CFSE trace in vitro and in vivo

Immunological assay

- FACS analysis (for 17 years of experiences)

- Intracellular cytokine staining and cytokine assay

- ELISA, ELISPOT

- making monoclonal antibody and purification

- cell purification by FACS, antibody cocktail and magnetic separator

- immunoblotting

Biochemistry

- SDS-PAGE

- Gel purification

- Affinity purification

Molecular biology

- cloning

- PCR

- Northern blot

LANGUAGE SKILLS

English

Korean

Japanese

PUBLICATIONS

Ishikawa, J., Kaisho, T., Tomizawa, H., Lee, B.O., Kobune, Y., Inazawa, J.,

Oritani, K., Itoh, M., Ochi, T., Ishihara, K. and Hirano, T. 1995.

Molecular cloning and chromosomal mapping of a bone marrow stromal cell

surface gene, BST-2, that may be involved in pre-B-cell growth. Genomics.

26:527-534.

Lee, B.O., Ishihara, K., Denno, K., Kobune, Y., Itoh, M., Muraoka, O.,

Kaisho, T., Sasaki, T., Ochi, T. and Hirano, T.. 1996. Elevated levels

of the soluble form of bone marrow stromal cell antigen 1 in the sera of

patients with severe rheumatoid arthritis. Arthritis Rheum. 39:629-637.

Okuyama, Y., Ishihara, K., Kimura, N., Hirata, Y., Sato, K., Itho, M., Lee,

B.O. and Hirano, T. 1996. Human BST-1 expressed on myeloid cells functions

as a receptor molecule. Biochem. Biophys. Res. Comm. 228:838-845.

Ishihara, K., Kobune, Y., Okuyama, Y., Itoh, M., Lee, B.O., Muraoka, O. and

Hirano, T. 1996. Stage-specific expression of mouse BST-1/BP-3 on the

early B and T cell progenitors prior to gene rearrangement of antigen

receptor. Int. Immunol. 8:1395-1404.

Ishihara, K., Okuyama, Y., Lee, B.O., Itoh, M., Nishikawa, K. and Hirano,

T. 1997. Structure, expression, and function of BST-1: its identity with

M05., in Leucocyte typing. VI:1086-1089.

Itoh, M., Ishihara, K., Hiroi, T., Lee, B.O., Maeda, H., Iijima, H.,

Yanagita, M., Kiyono, H. and Hirano, T. 1998. Deletion of BST-1 (CD157)

gene impaired systemic TI-2 antigen-induced IgG3 and mucosal TD antigen-

elicited IgA responses. J. Immunol. 161:3974-3983.

Lee, B.O., Haynes, L., Eaton, S.M., Swain, S.L., and Randall, T.D. 2002.

The biological outcome of CD40 signaling is dependent on the duration of

CD40 ligand expression: reciprocal regulation by IL-4 and IL-12. J. Exp.

Med. 196:693-704.

Lund, F.E., Partida-Sanchez, S., Lee, B.O., Kusser, K., Hartson, L., Hogan,

R.J., Woodland, D.L. and Randall, T.D. Lymphotoxin-a-deficient mice make

delayed, but effective, T and B cell responses to influenza. 2002. J.

Immunol. 169:5236-5243.

Lee, B.O., J. Moyron-Quiroz, J. Rangel-Moreno, K.L. Kusser, L. Hartson, F.

Sprague, F.E. Lund and T.D. Randall. 2003. CD40, but not CD154,

expression on B cells is necessary for optimal primary B cell responses.

2003 J. Immunol. 171(11): 5707-17

Lee, B.O., L. Hartson and T.D. Randall. 2003. Normal development and

function of CD40-deficient influenza-specific CD8 T cells in a CD40

sufficient environment. J. Exp. Med. 2003; 198(11): 1759-64

Lee, B.O., Javier Rangel-Moreno, Juan E. Moyron-Quiroz, Louise Hartson,

Frank Sprague, Frances E. Lund and T.D. Randall. 2004 CD4 T cell-

independent antibody responses promotes resolution of primary influenza

infection and helps to prevent reinfection. J. Immunol., Nov 2005; 175:

5827 - 5838.

Billaud JN, Peterson D, Lee BO, Maruyama T, Chen A, Sallberg M, Gardu o F,

Goldstein P, Hughes J, Jones J, Milich D. Advantages to the use of rodent

hepadnavirus core proteins as vaccine platforms. Vaccine. 2007 Feb

19;25(9):1593-606.

Denise A. Kaminski, Byung O. Lee, Sheri M. Eaton, Laura Haynes, and Troy D.

Randall CD28 and ICOS signaling can sustain CD154 expression on activated

T cells. 2009; Immunology, Jul;127(3):373-8 (First two authors equally

contributed)

Byung O. Lee, Amy Tucker, Lars Frelin, Matti Sallberg, Joyce Jones*, Cory

Peters, Jan Hughes, David Whitacre, Bryan Darsow, Darrell L. Peterson,

David R. Milich. Interaction of the Hepatitis B Core Antigen and the Innate

Immune System. 2009. J. Immunol, ;182: 6670 - 6681.

Lars Frelin, Therese Wahlstr m, Amy E. Tucker, Joyce Jones, Janice Hughes,

Byung O. Lee, Jean-Noel Billaud, Cory Peters, David Whitacre, Darrell

Peterson, and David R. Milich

A Mechanism To Explain the Selection of the Hepatitis e Antigen-Negative

Mutant during Chronic Hepatitis B Virus Infection. J. Virol., Feb 2009; 83:

1379 - 1392.

Whitacre DC, Lee BO, Milich DR. Use of hepadnavirus core proteins as

vaccine platforms. Expert Rev Vaccines. 2009 Nov;8(11): 1565-73

Byung O. Lee, Joyce E. Jones, Cory J. Peters, David Whitacre, Lars Frelin,

Janice Hughes, Won-Keun Kim and David R. Milich Identification of a unique

double negative (DN) Treg cell population, Immunology. 2011 Dec;134(4):434-

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