Assistant Ms Office

Saurabh Patil

** ******** ***. *** **, Boston, MA - 02115 | (617) 283 - 1863 | *******.*.*****@*****.***

PROFESSIONAL EXPERIENCE

Northeastern University, Boston, MA November 2009 - July 2011

Laboratory Assistant, ‘Center for Pharmaceutical Biotechnology and Nanomedicine’,

Laboratory of Dr. Vladimir Torchilin

Studied lysosomal targeting using (FITC)-Dextran loaded liposomes surface modified with Rhodamine B & Mitochondrial targeting using Rhodamine 123 conjugated nanocarriers

Maintained adherent mammalian cell lines and evaluated effect of liposomal formulation by cell cytotoxicity studies such as Cell titer Blue and LDH assay

Isolated intact mitochondria and lysosomes using density gradient ultracentrifugation

Performed Bradford and BCA assay to analyze total protein content in lysed cell fractions

Carried out SDS PAGE to quantitate total protein in cell lysate

Identified lysosomal fraction using fluorescence assay for β- Galactosidase activity

Analyzed binding capacity of modified 2C5 antibody using indirect ELISA

Confirmed accumulation of FITC-dextran and Rhodamine in cell fractions using fluorescence microscopy, spectrophotometry and flow cytometry (FACS)

Separated and purified ligand mixtures using size exclusion chromatography (SEC) and Thin layer chromatography

Formulated PEG-PE liposomal systems conjugated with ligands and characterized them for size using dynamic light scattering and for charge using zeta potential analysis

Compiled data from literature search and summarized results in an Excel report

¬¬MedImmune, LLC, Santa Clara, CA June 2010 - August 2010

Intern, Vaccine Process Biochemistry, Process Development Dept.

Project assigned: ‘Optimization and preparation of genomic and sheared DNA from Vero clone 51D11’

Worked extensively with QIAGEN QIAamp Maxi kit for genomic DNA isolation

Carried out detergent lysis and genomic DNA extraction from large scale batches of Vero suspension cells

Purified and concentrated DNA using RNase enzyme treatment, removal and ethanol/salt precipitation.

Confirmed DNA purity using agarose gel electrophoresis and analyzed gel images in Alpha Imager

Quantified DNA concentration in eluate using Picogreen dsDNA assay and NanoDrop spectrophotometer

Handled instruments such as Ultracentrifuge, probe sonicator and Nanosight particle size analyzer

Observed and assisted with viral harvest filtration using Tangential and Direct Flow filtration and protein purification using AKTA Explorer.

Successfully purified and delivered 1mg/mL target concentration of intact and sheared genomic DNA

Presented results at inter-departmental and company-wide presentation events

EDUCATION

Northeastern University, Boston, MA May 2011

Master of Science in Pharmaceutical Sciences

Specialization in Pharmaceutics and Drug Delivery Systems

University of Mumbai, India May 2009

Bachelor of Science in Pharmaceutical Sciences

Saurabh Patil

38 Westland Ave. Apt 52, Boston, MA - 02115 | (617) 283 - 1863 | *******.*.*****@*****.***

SKILL SET

Aseptic Mammalian cell culture techniques (BSL2), cell line maintenance, transfection

Molecular Biology techniques : DNA/RNA extraction, handling and storage of DNA preparations, agarose gel electrophoresis, ELISA, SDS-PAGE, RT-PCR, western blot; organelle isolation: lysosome and mitochondria; Protein assays: Bradford, BCA;

Enzyme assays: LDH, β- Galactosidase activity; flow cytometry, spectrophotometry, lyophillization

Dialysis, particle size analysis, TLC, column chromatography, ultracentrifugation, fluorescence microscopy

COMPUTER SKILLS

MS Office : Word, Excel, Powerpoint; SPSS, Graphpad

ACADEMIC PROJECTS

Poster presentation on ‘Development of liposomes modified with novel immunoconjugate of antinucleosome antibody 2C5 attached to PEG-PE via a pH sensitive bond’ at Northeastern University Research & Scholarship Expo, March 2010

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