Medical Supervisor

JIYANG JIANG, M.D., Ph.D.

MAIL: **** Lambertina Ln. Rockville, MD 20850

PHONE: 301-***-**** • E-mail ft2kav@r.postjobfree.com • U.S. citizen

SUMMARY

Senior Research Scientist with extensive research experiences in the areas of virology, immunology and pharmacology; broad knowledge in biochemistry, molecular and cell biology; abilities to independently design and operate experimental plan, evaluate and interpret test results, organize high-quality scientific writings and presentations; certificated medical education, hands-on experiences in clinical training; outstanding teamwork skills and effective communication and presentation skills.

RESEARCH EXPERIENCES

Employer Name: National Institutes of Health Dates: 06/07- present

Supervisor: Dr. Judith G. Levin 301-***-****(Permission to contact)

Title: IRTA Postdoctoral Fellowship

● Focusing on human immunodeficiency virus type 1 (HIV-1) capsid protein and analysis of the capsid protein’s role in early post-entry steps and the consequences for HIV-1 replication

● Demonstrated the linker region between NTD and CTD of HIV-1 capsid has a crucial role in virus assembly and formation of conical cores

Employer Name: Vanderbilt University, Nashville, TN Dates: 12/02- 05/07

Supervisor: Dr. Christopher Aiken 615-***-**** (Permission to contact)

Title: Postdoctoral Fellowship

● Focused on HIV-1 envelope function during maturation, demonstrated that coupling fusion to particle maturation is a common feature of HIV-1 Env proteins, regardless of HIV-1 co-receptor or usage and cell type

● Found evidence that the LLP1 domain in gp41 cytoplasmic tail plays an important role in regulating premature virion entry and the interaction of Pr55Gag with gp41 cytoplasmic tail is in sufficient to inhibit immature virions entry

Employer Name: Department of Immunology, Dates: 09/99- 07/02

Peking University Health Science Center, Beijing, P.R.China

Supervisor: Dr. Shusheng Xie,

● Focused on accelerating the process of hematopoietic and immune reconstitution after bone marrow transplantation

● Established tetracycline-inducible gene expression of bone marrow stromal cell line and found that the enginered bone marrow stromal cell could accelerate the process of hematopoietic and immune reconstitution after bone marrow transplantation

Employer Name: Department of Pharmacology Dates: 07/97- 08/99

Xinjiang Medical University, Xinjiang, P.R.China

Title: Lecturer

● Focused on the anti-inflammatory and anti-tumor activity of alkaloid compounds, identified of compounds derived from oriental traditional herbal medicine, including

berberin, harmain and harmalin, as anti-inflammatory or anti-tumor agents

● Taught courses in pharmacology and experimental pharmacology

MEDICAL EDUCATION

1994 M.B. (MD equivalent) Xinjiang Medical University, P.R. China

Dates: 09/89-07/94

GRADUATE EDUCATION

2002 Ph.D. in Immunology,

Peking University, health Science Center, Beijing, P.R. China Dates: 08/99-07/02

1997 M.S. in Pharmacology,

Xinjiang Medical University, Urumqi, P.R. China. Dates: 08/94-07/97

SELECTED JOB RELATED TRAINING

• Molecular & Cell Biology: Skillful in nucleic acid extraction and recombinant, real time PCR, western blotting, recombinant protein purification from bacterial and antibody purification from hybridomas cells.

• Microbiology: Rich experiences in working with retroviruses under BSL-3 biocontainment conditions, handling clinical infectious samples and radioactive materials. Proficient in DNA and RNA recombination, purification from bacterial, blood and tissue, virus production, purification and titration, conducting HIV-1 structure–function analysis including fusion, uncoating, RT activity, infectivity and replication assay.

• Immunology: Good at magnetic separation primary human cell, evaluation of antigen specific cellular immune responses, cell-based assay technologies, such as assays used for ADCC, cytokine secretion, proliferation and apoptosis; ELISA (p24, HIV Env, IL-6), immunohistochemical, immunofluorescence, flow cytometry based methods and T cell receptor repertoire diversity assays.

• Pharmacology: Good at developing experimental animal models in mice, rats and rabbits, evaluating drugs effectiveness, all routes of compound administration and blood sample collections and tissue harvesting. Skillful in pharmacokinetics and pharmacodynamics data analysis. Significant experiences in anti-HIV, anti-bacterial, anti-cancer drug screening and evaluation.

• Solid knowledge in biostatistics and data analysis. Proficient in using Word, Excel, PowerPoint, Adobe Illustrate, NIH Image, ImageQuant software, Odyssey Infrared Imaging System.

PUBLICATIONS

1. Jiang Jiyang, Ablan Sherimay, et al. The interdomain linker region of HIV-1 capsid protein is a critical determinant of proper core assembly and stability. Virology. Dec 2011,

421(2):253-65.

2. Jiang Jiyang, Christopher Aiken. Maturation-dependent human immunodeficiency

virus type 1 particle fusion requires a carboxyl-terminal region of the gp41 cytoplasmic tail. J Virol. 2007 Sep; 81(18):9999-10008.

3. Davis MR, Jiang Jiyang, Zhou J, Freed EO, Aiken C. A mutation in the human immunodeficiency virus type Gag protein destabilizes the interaction of the envelope protein subunits gp120 and gp41. J Virol. 2006 Mar; 80(5):2405-17.

4. Jiang Jiyang and Christopher Aiken. Maturation of the viral core enhances the fusion of HIV-1 particles with primary human T cells and monocyte-derived macrophages. Virology. 2006 Mar 15;346(2):460-8.

5. Marjorie Pion, Jean-Francois Arrighi, Jiang Jiyang, Christopher A Lundquist, Oliver Hartley, Christopher Aiken and Vincent Piguet. Analysis of HIV-1-X4 Fusion with Immature Dendritic Cells Identifies a Specific Restriction that Is Independent of CXCR4 Levels. J Invest Dermatol. 2006 Aug 17

6. VanCompernolle SE, Taylor RJ, Oswald-Richter K, Jiang Jiyang, Youree BE, Bowie JH, Tyler MJ, Conlon JM, Wade D, Aiken C, Dermody TS, KewalRamani VN, Rollins-Smith LA, Unutmaz D. Amphibian antimicrobial skin peptides potently inhibit HIV infection and transfer of virus from dendritic cells to T cells. J Virol. 2005 Sep; 79(18):11598-606.

7. Wyma DJ, Jiang Jiyang, Shi J, Zhou J, Lineberger JE, Miller MD, Aiken C. Coupling of human immunodeficiency virus type 1 fusion to virion maturation: a novel role of the gp41 cytoplasmic tail. J Virol. 2004 Apr; 78(7):3429-35.

8. Li A-L, Jiang JY, Xie SS. et al. Bone marrow stromal cell line co-transfected with IL-2 and IL-3 genes can accelerate restoration of T-cell immunity in allo-BMT mice. Chin Med J (Engl). 2004 Aug; 117(8): 1223-7

9. Jiang JY, Li A-L, Xie SS. The enhancing effects of IL-3 gene transfected murine bone marrow stromal cells on hematopoiesis under control of doxycycline. J Exp Hematol 2003; 11:335-340.

10. Jiang JY, Li A-L, et al. The improving effect of bone marrow stromal cell transfected with IL-3 gene on hematopoietic reconstitution in bone marrow transplantation of mice] Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2003 Dec; 11(6):633-8. Chinese.

11. Jiang JY, Li A-L , Liu C-Q , et al. The improving effect of gene engineered bone marrow stromal cell line which can induce IL-2 gene expression on immune reconstitution after bone marrow transplantation. Chinese J Microbiol Immunol ,2003 , 23(7):548-552

12. Wang Q, Jiang JY, Xie SS. Measured blood concentrations of Dox with high pressure in liquid chromatograph. Chinese Journal of Clinic Pharmacology. 2002,3:109-102

13. Jiang JY, Zhang J-M, Hao J, et al. The improving effect of bone marrow stromal cell

transfected IL-3 gene in allogeneic BMT mice. Chinese Journal of Hematology.

14. Jiang JY, Jin YZh, Hao J, et al. Accelerated hematopoletic function recovery by combined IL-3 and IL-6 gene therapy in allogeneic BMT mice. Chinese Journal of

Experimental Hematology. 2002; 10(5): 377-82.

15. Jiang JY, Jin Y-Zh, Hao J, et al. Improving immunologic function reconstitution by bone

marrow stromal cell transfected combined IL-3 and IL-6 gene in allogeneic BMT mice.

Shanghai Journal of Immunology. 2002,22(3): 153-156

16. Qin FH, Jiang JY, Jin YZ, et al. Accelerated immune reconstitution after bone marrow transplantation in mice by bone marrow stromal cell line transfected with IL-6 gene. Chin J Microbio Immunol (Chin) 2002; 22:546-550.

17. Jiang JY, Hao J, Xie SS. The accelerated immunologic function reconstitution by bone marrow stromal cell which reansfected IL-2 gene in allogeneic bone marrow transplantation. Chinese Journal of Immunology 2001, 12(6): 653-657

18. Jiang JY, Hao J, Xie SS. The improving effect of bone marrow stromal cell which transfected IL-3 gene on hematopoiesis in vito. Journal of Immunology. (Chinese) 2001,17(4): 285-288

19. Jiang JY, Lin H-Zh, et al, The anti-tumor activity of Harmain, Pharmacology and Clinics of Chinese Materia Medica. 2000.09.15; 16(S:S):5-6

20. Jiang JY, Geng D-Sh, Tuerxj T, et al. Anti-inflammatory effects and mechanism of berberine Chinese Pharmacological Bulletin 1998.10.20; 14(5): 434-437

21. Jiang JY, Liu F. The anti-inflammatory activity of Berbrine and its effects on Heymann nephritis. Information of the Chinese Pharmacological Society. 1998.11.30; 15(4): 36-37

22. Liu F, Jiang JY. The anti-inflammatory activity of Berbrine. Information of the Chinese Pharmacological Society 1998.11.30; 15(4): 11

INTERNATIONAL MEETINGS AND POSTERS

• 2010 Retrovirus in Cold Spring Harbor

Jiang, J. et al. Mutations in the hinge region of HIV-1 capsid that yield non-infectious virons with conical cores.

• 2005 Retrovirus in Cold Spring Harbor

Jiang, J. Aiken, C., The Requirement for Gag Cleavage in HIV-1 Particle Fusion is a General Feature of HIV-1 Env Proteins.

• 2004 Retrovirus in Cold Spring Harbor

Jiang, J. Aiken, C., Coreceptor-dependent requirement for HIV-1 maturation.

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