George W.Woodfield

856-***-****)

Email addresses: flxd9l@r.postjobfree.com

Over 12 years of cell and molecular based assay research and development with a results and challenge-driven attitude with additional experience in management of complex research projects and laboratory personnel. Extensive hands-on experience in development of cellular, molecular, and biochemical approaches in field of molecular oncology, including: Cell culture, gene expression profiling (Micro-Array analysis), validation with qPCR, and Next Gen qChIP analysis, strong analytical problem solving and solution generating abilities. Possesses leadership abilities with commitment to training, mentoring and fostering collaborative efforts among team members. Expressly detailed research technician with a commitment to organizational and communication skills.

Self-motivated and resilient Strong inter-personal skills

Accomplished Cell and Molecular research biologist Lab management experience

Skilled problem solver Results/Data based motivation

Skills Set

Biochemical Approaches (Protein–Protein Interactions)

● Recombinant protein expression and chromatography techniques: (IMAC, ion exchange, affinity, size exclusion, and reverse phase)

● Antibody-derived applications in cells, and tissues (western blot/IF/IHC/ELISA)

● Co- immuno-precipitation, GST- Pull-down and Far-western

● HPLC analysis, DNA/RNA synthesis, and native/denaturing PAGE

Cellular and Molecular Approaches

● Primary cell culture (primary adenocarcinomas, para-thyroid hyperplasia and normal breast epithelial), Breast, Colon, and Ovarian cancer cell lines, including passage and cell banking

● Transient and stable transfection DNA/RNAi based, virus gene transfer (Lentiviral and Adenoviral)

● ChIP Nex Gen sequencing on Illumina GA2 platform

● Gene cloning, sub-cloning, PCR mutagenesis, library construction and screening and 5’and 3’ RACE

● pGL-3 based reporter gene assays (luciferase)/(Renilla or GAL4)

● Flow Cytometry for cell sorting, apoptsis assay (Annexin V) or DNA cycle cycle (PI) on FACS DiVa, LSR II, and FACScan platforms (analysis on FLOWJO or Cell Quest software)

● Northern and Southern analysis

Mouse Genetics and Tumor Models

●Cre/Lox conditional MMTV TFAP2C knockout mouse model ● In vivo xenograft mouse tumor models

●Colony maintenance (breeding, genotyping, handling, bleeding, injections, and feeding/gavage)

Web Based Tools and Basic Statistics

●Sequence analysis and alignments on Sequencher, UCSC genome browser, NCBI Blast analyses

●Oncomine Data sets analyses

●Basic understanding of statistical analysis (SEM, t-tests, ANOVA). Ability to analyze, interpret data sets.

Professional Experience

University of Iowa, Carver College of Medicine, Iowa City, IA

Departments of Surgery and Biochemistry, Holden Comprehensive Cancer Center (2005-2010)

ASSISTANT RESEARCH SCIENTIST

● Involved in organizing bi-weekly team meetings instrumental for project progress

● Mentoring, training and supervising graduate students and post-doctoral fellows

● Foster collaborations with Principal Investigators inside and outside of the University of Iowa

● Responsible for lab management, purchasing, maintenance and organization

● Provides valued biochemical molecular and cellular experience

● Developed and implemented new ChIP Next Gen sequencing project

● Designed, managed and executed independent research projects

● Generating data coupling transcription factor binding with gene regulation in Breast cancer

● Data analysis software including: Sigma Plot, Graph Pad and Excel

● Published (Human Molecular Genetics 2010, Genes Chromosomes and Cancer 2010, Clinical Cancer Research 2009 and Cancer Research 2007)

Thomas Jefferson University College of Medicine, Philadelphia, PA

Departments of Surgery and Immunology, Kimmel Cancer Center (2004 -2005)

RESEARCH ASSOCIATE III

● Designed, managed and executed independent research projects

● Mentored, trained and supervised lab personnel including students and post-doctoral fellows

● Developed and implemented both biochemical and cellular assays

● Collaborated with the labs of C. Scott PhD and C. Croce MD at Thomas Jefferson University

● Responsible for laboratory management, including purchasing, equipment maintenance and laboratory organization

● Sequence analysis and web based software (Sequencher, Vector NTI, Primer Express, Oncomine)

● Generated data critical for NIH RO-1 funding in 2005

● Published (Oncogene, 2006 and Journal of Surgical Research, 2007)

Digital Gene Technologies, La Jolla, CA

Department of Integrative Biology (2001-2003)

RESEARCH ASSOCIATE I

● Developed and executed biochemical, molecular and cellular approaches to clone and express novel mRNA or protein fragments for the development of ribo-probes or antibodies for RNA ISH and or IHC

● Routinely performed cloning, IMAC protein purification, western, ISH, IHC and fluorescent microscopy

● Presented data in monthly group meetings

● Met or exceed research project goals

● Excelled in the team atmosphere, and also on individual based projects

● Received con-focal microscopy training

● Microsoft Office XP (Word, Excel, Outlook and Power Point)

San Diego State University, San Diego CA

Molecular Biology Institute (2000-2001)

RESEARCH ASSOCIATE III

● Researched Topoisomerase I and Cre recombinase catalysis and ligation enzyme kinetics

● Developed DNA suicide substrates for isolation reaction intermediates

● Performed HPLC, FPLC purification, mutagenesis, DNA synthesis, and modified base chemistries

● Published (Nucleic Acids Research, 2000)

Education

San Diego State University

M.S. in Cell and Molecular Biology 2001

Richard Stockton College of New Jersey

B.S. in Biology/Biochemistry 1996

Professional Memberships and Meetings

American Cancer Society 2007-present

Keystone Symposia, Molecular and Cellular Biology 2008

Publications

Calva-Cerqueira D., Dahaleleh F., Woodfeild G., Chinnathambi S., Nagy P., Laresn-Haidle J., Weigel R., and Howe J. “Discovery of the SMAD4 promoter and regulatory binding sites” Oncogene, submitted 11/2010.

Calva-Cerqueira D., Dahaleleh F., Woodfeild G., Chinnathambi S., Nagy P., Laresn-Haidle J., Weigel R., and Howe J. “Discovery of the BMPR1A promoter and germ-line mutations that cause juvenile polyposis”. Human Molecular Genetics, 9/24/2010.

Woodfield GW., Chen Y., Bair TB., Domann FE., and Weigel RJ. “ Identification of Primary gene targets of TFAP2C in hormone responsive breast carcinoma cells” Genes Chromosomes and Cancer. 2010 Oct; 49 (10):948-62

Woodfield GW., Hitchler MJ., Chen Y., Domann FE., and Weigel RJ. “ Interaction of TFAP2C with the estrogen receptor alpha promoter is controlled by chromatin structure.” Clinical Cancer Research. 2009 June 1; 15 (11):3672-9.

Woodfield GW., Horan AD., Chen Y., and Weigel RJ. “ TFAP2C controls hormone response in breast cancer cells through multiple pathways of estrogen signaling.” Cancer Research. 2007 Sept.15; 67 (18):8439-43.

McPherson LA., Woodfield GW., and Weigel RJ. “ AP2 transcription factors regulate expression of CRABPII in hormone responsive breast cancer.” Journal of Surgical Research. 2007 March.138; (1):71-8.

Stabach PR., Thiyagarajan MM., Woodfield GW., and Weigel RJ. “ AP2alpha alters the transcriptional activity and stability of p53.” Oncogene. 2006 April 6;25 (15):2148-59.

Woodfield GW., Chonghui C., Shuman S., and Burgin AB. “ Vaccinia topoisomerase and Cre recombinase catalyze direct ligation of activated DNA substrates containing a 3’-para-nitrophenyl phosphate ester.” Nucleic Acids Research. 2000 Oct.; 38 (18):3323-31.

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