Manager Technical Support

Vladimir V. KALASHNIKOV

Home address:

***** ***** *****

San Diego, CA 92130

Phone: 858-***-****

Cell: 858-***-****

E-mail: f75bdl@r.postjobfree.com

CAREER OBJECTIVE

Chemical or bio-pharmaceutical (GLP and GMP) manufacturing / R&D position

that has a potential for career growth and utilizes my knowledge of chemistry,

hands-on experience, scale-up and process improvement skills, and problem

solving skills.

EDUCATION

1970.1975 M.S./B.S. in chemistry (organic chemistry)

Moscow State University, Department of Chemistry, Moscow, Russia 117234

SUMMARY OF QUALIFICATIONS

Twenty years of peptide R&D, scale-up and GLP/cGMP production experience. 7 years of

oligonucleotide production. Key areas of specialization include:

• bio-organic chemistry

• peptide synthesis (solution and solid phase),

• oligonucleotide/DNA synthesis and purification; chemistry of nucleic acids

• peptide & protein modification and purification

• chemistry of side-chain, C- and N-protecting groups

• resins for solid phase peptide, oligonucleotide and organic syntheses

• synthetic (peptide, protein, DNA) vaccines

• conjugates, crosslinking agents

• chromatography of biomolecules (HPLC), amino acid analysis

PROFESSIONAL HISTORY AND SELECTED ACCOMPLISHMENTS

2012-now Sedna Industries, Inc.

QC Manager of Production.

Management of Production and Quality Control of proteins and enzymes from natural sources.

Work with CRO & CMO.

2009-2011 Aurora Fine Chemicals LLC, San Diego, CA

Senior Chemist.

Peptide R&D and production; solid- and solution-phase synthesis, modification and purification

of a wide spectrum of peptides and peptide-like molecules. Synthesis of reagents and resins for

solid phase peptide and organic syntheses. Conjugation of peptides with proteins and

oligonucleotides/DNA/RNA.

2001-2008 NeoMPS, Inc., San Diego, CA (now PolyPeptide Laboratories San Diego)

Manager, Technical Development.

Performed R&D for synthesis of modified, unnatural, radioactive, long, difficult, etc. peptides; peptide

analogs such as peptoids, aza-peptides, etc. Provided production leadership in custom peptide synthesis and

purification. Run pilot projects for future use in a GMP department. Lead in efforts for peptide synthesis

scale-up and process improvement. Bioconjugates.

2000.2001 American Peptide Company, Inc.; Sunnyvale, CA .

Senior Production Chemist

Provided technical leadership in synthesis on peptide synthesizers (Symphony and

robotic synthesizer). Provided technical support to peptide production management. Developed,

improved and implemented several production processes in peptide and bioconjugate production

that led to increased productivity and improved quality. Participated in special projects for

synthesis and purification of difficult, unusual peptides.

1996.2000 University of Virginia Biomolecular Research Facility, Charlottesville, VA

Research Associate

Provided scientific and technical support in custom peptide, bioconjugate and synthetic DNA

production. Diagnosed many peptide production problems and implemented many corrective and

preventive actions. Increased Facility’s peptide output from 40-50 peptides to more than 500 a year.

Reduced average peptide delivery time to 2-3 weeks. Performed R&D for p eptide synthesis,

modification and purification with specific attention to difficult, long, hydrophobic, modified,

branched, cyclic, phosphorylated (thiophosphorylated), glycosylated, isotope and dye labeled,

with unnatural amino acids, etc. peptides. Introduced a new protocol for syntheses of very

hydrophobic peptides. Provided technical support in QC of peptide and DNA synthesis (HPLC,

MALDI-TOF spectroscopy, amino acid analysis). Actively participated in the University’s inter

department projects for developing of peptide vaccines against melanoma (skin cancer).

Published a paper and presented posters at two international scientific meetings.

1994.1996 Department of Chemistry, University of Virginia, Charlottesville, VA

Research Fellow

Participated in molecular studies of DNA-binding proteins and bent DNA. Designed and

synthesized double stranded DNA molecules artificially bent with help of a specially synthesized

tether linker. Confirmed the specific structure of the synthesized molecule using electrophoresis.

Participated in development of specific DNA modification and crosslinking; design and synthesis

of special bifunctional crosslinking agents. The results were presented at 2 scientific conferences.

1991.1994 Vector-BioProduct Ltd., SPU Vector, Koltsovo, Novosibirsk region, Russia

Head of Laboratory of Peptide Synthesis.

Provided production management leadership in all aspects of custom peptide production

including synthesis, modification, purification, and QC. Supervised all and participated

personally in most of R&D projects. The laboratory included one Ph.D., two M.S. researchers,

and four specialists with B.S. degree. The Lab specialized in large scale peptide production using

an unique liquid-phase methodology. Major synthetic projects were: Adrenocorticotropic

Hormone, Luteinizing Hormone-Releasing Hormone, Oxytocin, Vasopressin and their analogs.

Scientific findings were published in 3 papers.

1983-1991 All-Union Research Institute of Molecular Biology, SPU Vector, Koltsovo,

Novosibirsk region, Russia

Senior Researcher

As a leader of a research group, provided management leadership in all aspects of custom

peptide production and actively participated in R&D projects which included peptide synthesis,

modification, purification, and analysis. The group was specialized in liquid-phase synthesis in

semi- and preparative scale of neuropeptides, fragments of viral proteins, etc. Participated in

inter department efforts for design and production of “chemical vaccines” against several viral

infections, including influenza. Published 10 research papers. Defended Ph.D. thesis in 1990.

1977-1983 All-Union Research Institute of Molecular Biology, SPU Vector, Koltsovo,

Novosibirsk region, Russia

Researcher

Provided technical support to oligonucleotide synthesis lab. Provided R&D support to

DNA production management. Participated in inter department efforts for syntheses of

biologically active genes of several proteins such as β -endorphin, interferons, etc. Selected

accomplishments are:

(1978) Awarded with special Ministry of Medical and Microbiological Industry Award

for active participation in pioneering synthesis of one of the first in the world biologically active

gene of a protein.

(1980) Developed a new method for synthetic oligonucleotide and DNA purification:

reversed-phase HPLC. Results were published in a research paper.

1975-1977 Chemistry Department, Moscow State University, Moscow, Russia

Research Fellow

Studied different approaches of oligonucleotide and DNA synthesis. Research activities

included solid- and solution-phase oligonucleotide synthesis; modification and purification of

nucleic acids.

SKILLS

Proficient in HPLC; RP, IP, IE, and SE chromatography of bio-molecules. Skilled

in mass-spectroscopy; IR, UV-Vis and NMR spectroscopy.

PUBLICATIONS

List of publications is available upon request.

REFERENCES

Justin Brandt, Supervisor, GMP Production, PolyPeptide Laboratories San Diego, Tel (858)

408 0885

Alexander Kutyrev, CEO, Aurora Fine Chemicals LLC, Tel:858-***-****

Firuz Shakoori, Director of Sales, American Peptide Co, Inc., Tel: 760-***-****

Shawn Lee, Ph.D., President & CEO, CPC Scientific, Tel: 877-***-****

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