Engineering Software

Degree: Master of Science ****-****

Universiy: Shahid beheshti university (Iran – Tehran www.sbu.ac.ir)

Qualification: Agriculture Engineering – Plant Biotechnology

M.Sc Thesis: 2010-2012

Cloning, expression, purification and characterization of 3-phytase in yeast

Thesis Summary:

I have honored to be one of the students of Dr. Seyed-Omid Ranaei-Siadat for doing my thesis at the Faculty of New. Technologies and Energy engineering. I transformed hansenula polymorpha with a suitable vector that have phytase for its

LABORATORY SKILLS

• Basic Laboratory Skills (Restriction Enzyme Digestion, PCR, Electrophoresis)

• Gene Cloning, Colony PCR Screening from different clones and quick check screening

• Isolation of DNA, Plasmid, Protein and RNA, RT-PCR, cDNA synthesis

• Bacterial and Yeast Transformation methods

• Familiar with the concepts of HPLC

• SDS and native gel electrophoresis

• Familiar with Real time pcr and souther blot

Bioinformatics SKILLS

• More than basic and routine skills in using few operating systems and many software's

• DNA analyzing computer software's,Vector NTI Advance 11.0, OLigo 7, Matlab (bioinformatics toolbox)

• Word Processing, Microsoft Office

• statistics Software's (Prism, MSTAT)

• Endnote Ver10

Supervisors

Dr. Seyed-Omid Ranaei-Siadat

Address Nanobiotechnology engineering lab., Biotechnology Group, Faculty of New

Technologies and Energy engineering, Shahid Beheshti University, Tehran, Iran

Website: http://www.irnbt.com

Tell: +982*********

E – Mail: ********@***.**.** & *******@*****.***

Degree: Bachelor of Science 2000-2004

University: Zabol university (Iran – zabol www.uoz.ac.ir)

Qualification: Agriculture Engineering – Plant Breeding

Professional affiliations

1- I am a member of Iranian Biotechnology Society (www.iribs.org)

2- Instructor to the Workshop on "Primer Design & PCR Principles"

Objectives:

• An introduction to PCR and molecular mechanism of the reactions

• Principles of primer design

• Familiarity with Nested PCR, Hot Start PCR, SOE PCR and Touchdown PCR

• An introduction to “Oligo” software for primer design

• Familiarity with application of PCR in various sciences

• PCR Troubleshooting

3-- Instructor to the Workshop on “Molecular Cloning & Bacterial Transformation”

Objectives:

• Different applications of genetic engineering

• Digestion, ligation and gene cloning in different vectors

• DNA gel electrophoresis

• Bacterial transformation

• Plasmid extraction and purification

• Confirmation of gene cloning by colony-PCR

4- Poster in the Third Congress on Biosafety and Genetic Engineering

5-15th person in student Olympiad in 2010

6-9th person in student Olympiad in 2011

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