Research Graduate
Resume:
YOGESH T. GANESAN
********@*****.***
OBJECTIVE
* Seeking a full-time position in the field of biotechnology, that will utilize my strong research experience in cell biology, molecular biology and biochemistry.
ACADEMIC QUALIFICATIONS
* M.S., Masters Degree in Biotechnology, Polytechnic Institute of NYU, NY, January 2011. CGPA: 3.9/4.0
* B. Tech., Bachelor's Degree in Industrial Biotechnology, Anna University, Chennai, India, June 2008 CGPA: 8.3/10
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RESEARCH EXPERIENCE
* Research Assistant, NYU College of Dentistry, NYU - September 2009 to December 2010.
* Studied the interaction between apoptotic and anti-apoptotic Bcl-2 family proteins in mammalian cell lines, which resulted in identifying the key steps in the interaction between the Bcl-2 family proteins.
* Cell tissue culture maintenance of FL5.12 cell lines.
* Developed an immuno-detection assay for BAX with flow cytometry to complement immuno-detection assay by Western blotting.
* Developed an ELISA assay for detection of BAX in mammalian cells which detects the conformational change in BAX.
* Research Assistant and Lab Manager, Chemical & Biological Sciences Department, Polytechnic Institute of NYU - February 2009 to August 2009.
* Studied the incorporation of unnatural amino acids in phosphotriesterase enzyme and investigated their activity and stability.
* Managed orders in the laboratory, storage, cataloging and handling of the chemicals that helped run the experiments in the laboratory smoothly and effectively.
* Maintained accounts and expenses of the laboratory that helped the professor keep track of the monthly expenses.
* Research Assistant, Centre for Biotechnology, Anna University, Chennai, India - December 2007 to May 2008.
Undergraduate Senior Research Thesis titled "A Comparative Study of Mycobacterium leprae and Mycobacterium tuberculosis"
* Analyzed the genome of M. tuberculosis and M. leprae using Standalone BLAST.
* Identified two previously non-annotated leprae-specific proteins ML0576 and ML1275, having a role in phosphoenolpyruvate carboxylase activity and prolipoprotein diacylglyceryl transferase activity in M. leprae.
* Research Internship, Indian Institute of Chemical Biology, Kolkata, India - May 2007 to July 2007 "Regulation of differentiation associated events by cyclic-AMP response regulatory proteins in Leishmania donovani"
* Identified and characterized LdPDEA1, a low-Km, cAMP-specific phopshodiesterase enzyme of the protozoan parasite, Leishmania donavani. The enzyme is involved in regulation of cellular processes by controlling cAMP levels.
* Determined the role of cAMP and phosphodiesterase A in the differentiation processes and polyamine biosynthesis in the life cycle of L. donovani.
* Research Internship, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India - May 2006 to July 2006.
* NusG is an important part of the transcription termination complex in Escherichia coli. It interacts with RNA polymerase and Rho factor in regulating the transcription of proteins in E. coli.
* In order to identify the site of interaction of NusG with Rho factor, cysteine derivatives of NusG was cloned using site directed mutagenesis (SDM), which could eventually be made to form a complex with Fe-BABE (p-Bromoacetamidobenzyl-EDTA, iron (III) chelate) used in protein foot-printing and labeling.
LABORATORY SKILL SETS
* Molecular Biology: Gel Electrophoresis, DNA and RNA handling and isolation, PCR, RT-PCR, mutagenesis, bacterial (E. coli) transformation, molecular DNA subcloning, protein expression and purification, Immobilized Metal Affinity Chromatography, FPLC (AKTA) and HPLC.
* Immunology/Cell-based Techniques: SDS-PAGE, Western blotting, flow cytometry (FACS), ELISA (96-well plates), Immunocytochemistry, Immunoprecipitation.
* Cell tissue culture: Culturing and maintaining sterile FL5.12 (mammalian) cell lines, an interleukin (IL)-3-dependent murine prolymphocytic cell line.
* Microbiological Techniques: Sterilization, aseptic preparation of LB agar media, media preparation, streaking and inoculation of bacteria.
* Programming Languages: C, R (for microarray data analysis), Perl, Basic bioinformatics tools, Computational analysis, Genomics, comparative genomics using Standalone BLAST, MS Office (Excel, Power point, Word), HTML (Website Maintenance) and Visual Basics.
* Maintenance and calibration of equipments, waste management and chemical inventories.
ACADEMIC ACHIEVEMENTS
* Co-authored a paper for the EMBO report, entitled "Bcl-xL increases abundance of BAX at the level of mitochondria" - In progress, to be completed by March 2011.
* Co-authored a paper for the Laboratory Investigation journal, entitled "A specific quantification of mitochondrial BAX by ELISA" - In progress, to be completed by June 2011.
* Poster Presentation on "Engineering of Phosphotriesterase to Detoxify Organophosphates in Extreme Environments" at the 3rd Annual Advances in Biomolecular Engineering: Protein Design Symposium, The New York Academy of Sciences, New York, 2009.
* Poster Presentation on "An ELISA for rapid quantification of the tumor-suppresor BAX" at the International Association for Dental Research - 89th General Session and Exhibition (March 16-19, 2011) to be held in San Diego, CA.
* Co-Author for the Poster Presentation on "Interactions between BCL-2, BCL-xL, and BAX at the Mitochondria: Keep Your Friends close, but your Enemies Closer" at the 55th Biophysical Society Annual Meeting (March 5 -9, 2011) to be held in Baltimore, Maryland.
* Indian Academy Of Sciences Fellowship 2006 and 2007.
EXTRA-CURRICULAR ACTIVITIES
* Teaching Assistant, Department of Basic Sciences, NYU College of Dentistry from April 2010 to July 2010.
* An active organizer and a member of the scientific committee of "Biotechcellence 2007", a National level Biotechnology Symposium organized at Anna University, Chennai, India, 2007.
* An active Organizer of The National Science Day held at Anna University, Chennai, India, 2006.
REFERENCES
Available upon request.
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