Algae physiologist, applied phycologist, biochemist

WITT, FEDERICO GUILLERMO

Address: Avda. de los Almogávares 72, 1º 1, E-14006, Córdoba, Spain

E-mail: **********@*****.***

Skype: fgwitt

WORK EXPERIENCE

- Research Associate / Spanish National Research Council (CSIC), at the Centre for Environmental Sciences (CCMA-CSIC, Madrid). 2003- (last years non-contractual relationship)

- Research Associate / Ministry of Science and Technology and University of Córdoba, at the University of Córdoba (UCO, Córdoba) and the Centre of Biological Research (CIB-CSIC, Madrid). 2001-2002

- Postdoctoral Fellow / Community of Madrid (CM), at the CIB-CSIC, Madrid, 2000

- Research Associate / German Research Council, DFG, at the Darmstadt University of Technology (TUD, Germany), 1995-1996 and 1998-1999

- Postdoctoral Fellow / Alexander von Humboldt Foundation, at the TUD, Germany 1996-1998

- PhD Fellow / Ministry of Education and Science, at the CIB-CSIC, Madrid, 1991-1994

- PhD Fellow / Autonomous University of Madrid (UAM), at the CIB-CSIC, Madrid, oct-dec 1990

Other occupational activities

Translator (Scientific English and German to Spanish), proofreader and editor. Freelance, agencies and publishers. 2007-2011

PATENT: ES 2 251 286. Inland water treatment by cultures of green filamentous macroalgae that take up and recycle nutrients, fix heavy metals and, at the same time, produce plant biomass. International Classification: C02F 3/32 (2006.01) (Biological treatment of water, waste water, or sewage - characterised by the animals or plants used, e.g. algae). Granted and published in 2007.

EDUCATION

Ph.D. in Biology (1990-1995)

Autonomous University of Madrid, Faculty of Sciences.

Dissertation: Photoregulation of monovalent anion uptake by the green alga Monoraphidium braunii. Action spectra of nitrate and chloride transport processes.

M.Sc. in Biology (1985-1990)

Autonomous University of Madrid, Faculty of Sciences.

Specialty of Biochemistry and Molecular Biology.

COURSES, TRAINING

Environmental Technician: Knowledge and Management of the Resources. 1999-2000. Institute for Employment and Learning of the Town of Madrid

Techniques in Photosynthesis. Postgraduate Experimental Course. 1993. University of Seville

Doctorate (postgraduate) courses (Autonomous University of Madrid):

-Plant Molecular Biology. 1990-1991

-Algal Biotechnology. 1990-1991

-Optimization of Nitrogen Nutrition. Basis and Applications. 1990-1991

-Coevolution of Plants and Insects. 1991-1992

-Microbial Ecosystems in Extreme Environments. 1991-1992

-Techniques for Microalgae Physiology and Biotechnology. 1992-1993

RESEARCH PROJECTS in which I took part

-Interactions between nitrogen and hydrogen metabolisms in phototrophs and in non-photosynthetic, nitrogen fixing organisms.

FUNDING ORGANIZATION: Spanish Directorate-General for Scientific and Technological Research (DGICYT)

1988-1991

PI: Pedro J. Aparicio

-Activity modulation of biological systems catalyzing electron transport processes

FUNDING ORGANIZATION: DGICYT

1988-1992

PI: Juan Manuel Ramírez

-Biosensor for nitrate: Nitrate reductase-based electrode

FUNDING ORGANIZATION: Community of Madrid

1990-1992

PI: Víctor Manuel Fernández

-Molecular Biology of nitrate reduction in bacteria and green algae.

FUNDING ORGANIZATION: DGICYT

1993-1995

PI: Francisco Castillo.

-Photoregulation of nitrate utilization in cyanobacteria, green algae and higher plants. Molecular characterization of cyanobacterial nitrate reductase and nitrate transport systems.

FUNDING ORGANIZATION: DGICYT

1993-1996

PI: Pedro J. Aparicio.

-Molekulare Ökophysiologie der Pflanzen. Teilprojekt A2. (Translation from German: Plant Molecular Ecophysiology. Part A2 of the project).

FUNDING ORGANIZATION: Deutsche Forschungsgemeinshaft (German Research Council).

1993-2001

PI: Wolfram R. Ullrich.

-Photoregulation of anion transport and nitrate reduction in microalgae.

FUNDING ORGANIZATION: DGICYT

1997-2000

PI: Pedro J. Aparicio.

-Molecular Biology of inorganic nitrogen reduction in bacteria and green algae.

FUNDING ORGANIZATION: DGICYT

1999-2002

PI: Emilio Fernández.

-Culture of the green alga Hydrodictyon for the possible treatment of waters in the region.

FUNDING ORGANIZATION: Community of Madrid

Jan-Dec 2000

PI: Pedro J. Aparicio.

-Studies on inland water treatment technologies based on green algae. Characterization of the systems involved in the uptake of nutrients and/or contaminants.

FUNDING ORGANIZATION: Ramón Areces Foundation

2002-2005

PI: Miguel Ángel Quiñones.

I was the director of the project:

-Water treatment systems based on green algae for rural areas.

FUNDING ORGANIZATION: Regional Government of Castile-La Mancha

2001-2004

PI: Federico G. Witt

RESEARCH LINES

- Proteins involved in assimilatory nitrate reduction by microalgae and higher plants. Kinetic and molecular characterization of cytosolic and plasma membrane-bound nitrate reductase isoforms. Isolation and identification of membrane fractions containing plasma membrane-bound nitrate reductase (PMNR) in dinoflagellates and higher plants (tobacco, corn, barley and wild grasses). Immunolocalization of PMNR and identification of two plasma membranes in dinoflagellate cells. Optimization of the in vitro conditions and extraction media to achieve maximal nitrate reductase activities. Comparison between cytosolic (soluble) NR and PMNR. Studies on the physiological factors that affect their in vivo activities (by both de novo synthesis/degradation and posttranslational modifications): nutritional status, temperature, age of the culture, light/dark ratio, light intensity, etc. Ecological role of these findings in relation to red tides and harmful algal blooms: causes of the waterblooms of Peridinium gatunense in the Sea of Galilee.

- Bioremediation of eutrophic waters and wastewater treatment with filamentous algae. Development of a technology focused towards the reclamation of water contaminated by urban sewage, agricultural runoff and/or livestock operations, by the controlled culture of filamentous algae. Development of photobioreactors, pilot plants and full-scale facilities.

- Photoregulation of monovalent anion uptakes by unicellular green algae. Identification of the blue-UVA photoreceptor involved in the positive signal for their transport. Kinetic characterization of the transport mechanisms. Determination of the environmental factors (temperature, light intensity, pH, C and N sources, light/dark cycles, etc.) that promote the synthesis of the photoreceptor and/or the membrane carrier proteins. Physiological role of the photoregulation in C fixation and N assimilatory reduction. Effects on C/N ratio under different enviromental conditions.

RESEARCH FIELDS

- Plant Cell Biology: Protein compartmentalization in different plant cell organelles. Cell lysis, subcellular fractionation, isolation of membrane vesicles, identification of extracts, protein extraction from membranes…

- Protein Biochemistry: protein extraction (soluble and membrane fractions), isolation, purification, immunochemical identification, molecular characterization, hydrophobicity, redox properties, enzyme activity…

- Bioenergetics: Driving forces for anion transport processes across plant plasma membranes. Activity and inhibition kinetics of different types of ATPases. Redox enzymes. Key proteins of the mitochondrial and tylakoidal e- transport chains. Photosynthesis/respiration.

- Photochemistry and Photobiology: Flavoprotein and pterin (Molybdenum cofactor) photochemistry. Blue-UVA plant photoreceptor (cryptochrome). Action spectra of nitrate and bicarbonate uptakes. Photomodulation of protein activities.

- Plant (algal) Physiology: Regulation of enzyme activities and transport processes involved in inorganic nitrogen assimilation. Role and relative significance of C, N and P availability for algal culture growth. Photosynthetic and respiratory pathways: enzymes connecting C and N metabolisms.

- Membrane biochemistry: Properties of membrane vesicles from plants and algae. Sidedness and enzyme latency. Isolation of different membrane fractions and their identification by means of marker enzymes. Solubilization, purification and characterization of plasma membrane bound nitrate reductase. Comparison with cytosolic (soluble) NR isoform.

- Water treatment: algae-based water treatment plants. Use of plant biomass.

METHODS AND TECHNIQUES EXPERIENCED

- Immunological techniques:

• Production of polyclonal antibodies (antisera from inoculated New Zealand rabbits) and Fab fragments.

• Immunoprecipitation with Protein A from Staphylococcus aureus, Cowan strain.

• Indirect immunofluorescence with antibodies conjugated to fluorochromes (FITC or TRITC).

• Western Blotting and Dot Blotting.

- Chromatographic techniques: Preparative and analytical column chromatographies. Classic and automatic (computerized, monitorized) methods (HPLC and FPLC). Affinity chromatographies (lectin, Blue-Sepharose, Protein A), size-exclusion chromatography (gel filtration) and ion-exchange chromatography.

- Polyacrylamide Gel Electrophoresis (PAGE): Denaturing conditions (SDS-PAGE) and native conditions (Native-PAGE). Zymographies.

- Culture media and techniques for a great variety of algae species, including mutant strains. Also fungi, protozoa and higher plants. Use of phytotrons, growth chambers and an automatic greenhouse. Design and operation of pilot plants and photobioreactors (PBR) for biomass exploitation.

- Analytical methods:

• Cuantitative measurements of proteins and nucleic acids.

• Ion (inorganic and organic) cuantitative and qualitative measurements: spectrophotometric, enzymatic, HPLC, ion-selective electrodes, etc.

• Identification and quantification of plant pigments.

- Polarographic (Clark electrodes for O2) and potentiometric (ion-selective electrodes, automatic titrations, etc.) techniques.

- Methods in Photochemistry and Photobiology:

• Use of precision optics: filters (absorption, interference, dioptric, cut-off, cut-on, neutral), opto-mechanics, monochromators, fiber optics, etc.

• Choice of proper light sources: arc lamps, tungsten incandescent lamps, LED technology, fluorescent lamps...

• Instruments for radiation measurement (quantum sensors, thermopile-type pyranometers), etc.

• Fluorescence detection.

• Action spectroscopy, absorption spectroscopy, fluorescence spectroscopy...

- Methods in Enzymology and Protein Chemistry. Extraction of soluble and membrane associated proteins after cell lysis and fractionation, protein purification and concentration, enzyme activity measurements, partial activities in multidomain enzymes, determination of protein molecular weight, Stokes radius, number of subunits, kinetic parameters (Km, Vmax, activation energy, Q10, etc.), hydrophobicity tests with different detergents and separation of proteins by Hydrophobic Interaction Chromatography (HIC).

- Centrifugation. Preparative and analytic. Ultracentrifugation, differential centrifugation and density gradient centrifugation (separation by size -rate zonal centrifugation- or by density -isopycnic centrifugation- with sucrose, silica sols like Percoll, or polysaccharides like Ficoll).

- Techniques with biomembranes:

• Isolation of right-side-out and/or inside-out plant membrane vesicles, after cell lysis and fractionation (differential centrifugation), and purification by density gradient centrifugation (sucrose or Percoll) and/or separation in aqueous (PEG-dextrane) two-phase systems or affinity chromatography with concanavalin A.

• Identification of isolated membrane vesicles by enzyme markers and other standard procedures.

• Isolation of intact chloroplasts and mitochondria and tests for integrity criteria.

• Determination of the orientation of the membrane vesicles (sidedness) after the latencies of the marker enzymes.

• Integral membrane proteins extraction by solubilization and temperature-induced phase separation with Triton X-114.

- Optical Microscopy (Light Microscopy): bright field, dark field, phase contrast (Nomarski interference), epifluorescence microscopy (for immunolocalization).

- Ecological and environmental techniques and methods:

• Standard methods for the physico-chemical analysis of water (field and laboratory measurements of ion concentrations, pH, oxygen, CO2, salinity, alkalinity, OBD, OQD, solids [total, suspended, dissolved], Secchi depth, etc.)

• Microbiological and biological examinations. Identification of phytoplankton, benthos and periphyton, zooplankton, microinvertebrates, etc.

• Monitoring and recording of environmental and meteorological parameters (wind speed and direction, air temperature, air pressure, air humidity, precipitation, haze and contents of the air, solar radiation [hours/day, irradiation energy], atmospheric pressure, etc.).

• Algal toxicity tests.

• Design, maintenance and operation of an outdoor pilot plant for water treatment.

LANGUAGES AND SOFTWARE SKILLS

Spanish: Mother tongue.

English: Good reading, understanding, writing and speaking skills.

German: Good reading and understanding skills. Medium writing and speaking skills.

-Certificate of Scientific English Course (Autonomous University of Madrid).

-TOEFL.

-Three levels of Deutsch als Fremdsprache at the Volkshochschule Heidelberg.

-More than five years experience as scientific translator (English and German to Spanish).

I can manage any program or application running in Windows OS (wide experience with word processors, Optical Character Recognition tools [OCR, like Abby FineReader], Computer-Assisted Translation tools [CAT, like SDL Trados], databases, spreadsheets, image editors, graphics software, file and mail managers, Web browsers, Content Management Systems [CMS as Joomla (Mambo), PHP-Nuke, WordPress], HTML editors, FTP tools, etc.).

CONGRESSES, MEETINGS, SYMPOSIA, WORKSHOPS (only communications to scientific events are listed)

International:

1. Blue light regulation of nitrate utilization by green algae. P.J. Aparicio and F.G. Witt. International Conference on Carbon and Nitrogen Metabolism in Photosynthesis. Pushchino, C.I.S., July 22-26 1991. Plenary Conference

2. Nitrate dependent alkalinisation induced by blue light in the outer medium of Monoraphidium braunii. F.G. Witt, T. Balandín and P.J. Aparicio. IV Portuguese-Spanish Biochemistry Congress. Abstracts 2 P38-Tu. Póvoa de Varzim, Portugal, 1991. Poster

3. The photoregulation of the inorganic nitrogen metabolism in Monoraphidium braunii. UV and/or blue light dependent monovalent anion uptake. F.G. Witt and P.J. Aparicio. 8th Congress of the Federation of European Societies of Plant Physiology. Physiologia Plantarum vol. 85(3) Part 2, A39. Amberes, Belgium, 1992. Poster

4. UV and/or blue light dependent monovalent anion uptake by Monoraphidium braunii. P.J. Aparicio and F.G. Witt. Third In¬ternational Symposium on Inorganic Nitrogen Assimilation. Abstracts, A-5. Tiberias, Israel, 1992. Closing Conference by P. Aparicio

5. UV and/or blue light requirement for both monovalent anion uptake and nitrate assimilation in Monoraphidium braunii. P.J. Aparicio and F.G. Witt. XIX Panamerican Association of Biochemical Societies Congress. Abstracts, PLS-1R. Ixtapa, Mexico, 1992. Oral Commun

6. Photoregulation of the uptake of NO3-, NO2- and Cl- by the green alga Monoraphidium braunii. Pedro J. Aparicio and Federico G. Witt. International Symposium on Plant Membrane Biology. Abstracts, L29. Lund, Suecia, 1994. Oral Commun

7. Flavin type action spectra of the NO3- and Cl- uptake in Monoraphidium braunii. F.G. Witt and P.J. Aparicio. 9th Congress of the Federation of European Societies of Plant Physiology. Biologia Plantarum, 36 (suppl.) S56, 03 23. Brno, Czech Republic, 1994. Oral Commun

8. Action spectra of the uptake of Cl- and NO3- by the green alga Monoraphidium braunii. Witt, Federico G., Quiñones, Miguel A. and Aparicio, Pedro J. II Algal Photobiology Workshop. Málaga, Oct 10-14 1994. Oral Commun

9. Regulation of nitrate uptake in unicellular algae by blue light: Differences between Monoraphidium, Hydrodictyon and Peridinium. Ullrich W.R., Aparicio P.J., Lazarová J., Witt F.G. and Ullrich-Eberius C.I. International Symposium of the Sonderforschungsbereich 199. Ecophysiology of Plants: Molecules, Membranes and Mechanisms. Abstracts pp. 64-65. Ebernburg (Nahe), Germany. 1995. Poster

10. Photoregulation of the monovalent anion uptake by Monoraphidium braunii. Aparicio P.J., Witt F.G. and Quiñones M.A. Fourth International Symposium on Inorganic Nitrogen Assimilation and First Fohs Biostress Symposium. Abstracts p. 12. Seeheim/Darmstadt, Germany. 1995. Oral Communication

11. Simultaneous restoration of the NO3- and Cl- transport systems in Monoraphidium braunii cells grown in NH4+ medium or in cells coming from dark periods. Witt, Federico G. and Aparicio, Pedro J. Fourth International Symposium on Inorganic Nitrogen Assimilation and First Fohs Biostress Symposium. Abstracts p. 138. Darmstadt, Germany. 1995. Poster

12. Las radiaciones monocromáticas y el metabolismo del nitrógeno en Monoraphidium braunii. (Monochromatic radiations and nitrogen metabolism in Monoraphidium braunii). Aparicio, P.J., Witt, F.G. and Quiñones, M.A. IV Congresso Luso-Espanhol de Fisiologia Vegetal (IV Portuguese-Spanish Plant Physiology Congress). Abstracts p. 117. Estoril, Portugal. 1995. Poster

13. Blue light activation of the monovalent anion uptake by Monoraphidium braunii. Witt, F.G., Quiñones, M.A. and Aparicio P.J. European Symposium on Photomorphogenesis in Plants. Abstracts p. 126. Sitges, Barcelona, Spain. 1995. Poster

14. Regulatory strategies for the photosynthetic nitrate assimilation in unicellular green algae: Giant-celled algae versus microalgae. F.G. Witt. Photosynthese-Workshop (Workshop on Photosynthesis). Darmstadt, Germany. 1997. Oral Commun

15. Nitrate reductase in the dinoflagellate Peridinium gatunense. Witt F.G. and Ullrich W.R. Second International Symposium of the Sonderforschungsbereich 199. Ecophysiology of Plants: Molecules, Membranes and Mechanisms. Abstracts, add. Ebernburg (Nahe), Germany. 1997. Poster

16. Incorporación de nitrato y bicarbonato en Monoraphidium braunii: dos transportadores independientes regulados por un mismo fotorreceptor de luz azul (Nitrate and bicarbonate uptakes in Monoraphidium braunii: Two independent carriers regulated by the same blue light photoreceptor). Aparicio P.J., Quiñones M.A., Giráldez N. and Witt F.G. V Reunión Nacional de Metabolismo del Nitrógeno. Biochemical Society. First Joint Meeting of the Nitrogen Metabolism Groups. Abstracts p. 37. Torremolinos, Málaga, Spain. 1999. Closing Conference

17. Nitrate Reductase Forms in the Dinoflagellate Peridinium gatunense. Witt F.G. Humboldt-kolloquium für Humboldtianer aus Portugal und Spanien anläßlich des 200 Jahrestages der abreise Alexander von Humboldts nach Südamerika (Translation from German: Humboldt colloquium for humboldtians from Portugal and Spain commemorating the 200th anniversary of the departure of Alexander von Humboldt to South America. La Coruña, Spain. 1999. Oral Communication

18. Utilisation of Hydrodictyon reticulatum for N and P removal from rural waters. Witt F.G., Mora C., Quiñones M.A. and Aparicio P.J. 1st Congress of the International Society for Applied Phycology / 9th International Conference on Applied Algology. Abstracts p. 186. Aguadulce, Almería, Spain. 2002. Oral Commun

19. Hydrodictyon reticulatum: a filamentous alga for N and P removal from rural waters. Witt F.G., Quiñones M.A. and Aparicio P.J. NAMGA2002. 5th International Symposium. Nitrate Assimilation: Molecular and Genetic Aspects. Abstracts 122-123. Córdoba, Spain. 2002. Oral Commun and poster

20. Blue light regulation of anion transport in green microalgae: Induction and activation of plasma membrane transport systems. Witt F.G., Aparicio P.J. and Quiñones M.A. The Blue Light Syndrome IV. International meeting on UV/blue light: Perception and responses in plants and microorganisms. Joint meeting: Research meeting of National Institute for Basic Biology. Abstracts p. 56. Okazaki, Japan. 2002. Poster

21. Singularidad Tecnológica (Technological Singularity). Federico G. Witt. Encuentro Fractal'09. Medellín, Colombia. 2009. Conference

22. Manipulación genética (Gene Manipulation). Federico G. Witt. Encuentro Fractal'09. Medellín, Colombia. 2009. Conference

National:

1.Alcalinización del medio externo dependiente de nitrato, nitrito y cloruro inducida por luz azul en Monoraphidium braunii (Nitrate-, nitrite- and chloride-dependent Monoraphidium braunii external medium alkalizations induced by blue light). P.J. Aparicio and F.G. Witt. IX Reunión Nacional de la Sociedad Española de Fisiología Vegetal, y II Congreso Hispano-Luso de Fisiología Vegetal (IX National Meeting of the Spanish Society of Plant Physiology, and II Spanish-Portuguese Congress of Plant Physiology) Resúmenes, p. 138. Madrid, Spain, 1991. Poster

2.Las radiaciones ultravioleta cercana y azul, señales reguladoras de la asimilación del nitrógeno inorgánico en organismos fotosintéticos eucarióticos (Blue and near UV radiations: regulatory signals for inorganic nitrogen assimilation in photosynthetic eucaryotes). P.J. Aparicio and F.G. Witt. III Congreso de la Sociedad de Biofísica de España (III Congress of the Spanish Biophysical Society). Abstracts, p. 54. Madrid, Spain, 1991. Oral Commun

3.Efecto de la luz azul en la entrada de aniones monovalentes en Monoraphidium braunii (Effect of blue light on the monovalent anions uptake by Monoraphidium braunii). F.G. Witt y P.J. Aparicio. III Congreso de la Sociedad de Biofísica de España (III Congress of the Spanish Biophysical Society). Madrid, Spain, 1991. Poster nº 63

4.Fotorregulación de la entrada de NO3-, NO2- y Cl- en Monoraphidium braunii (Photoregulation of the uptakes of NO3-, NO2- and Cl- by Monoraphidium braunii). F.G. Witt and P.J. Aparicio. I Reunión Nacional del Grupo del Metabolismo del Nitrógeno, Sociedad Española de Bioquímica (1st National Meeting of the Nitrogen Metabolism Group, Spanish Society of Biochemistry). Abstracts, p. 19. Málaga, Spain, 1992. Oral Communication

5.Fotorregulación de la incorporación de NO3-, NO2- y Cl- por el alga verde Monoraphidium braunii (Photoregulation of the uptakes of NO3-, NO2- and Cl- by the green alga Monoraphidium braunii). P.J. Aparicio and F.G. Witt. X Reunión Nacional de la Sociedad Española de Fisiología Vegetal. III Congreso Hispano-Portugués de Fisiología Vegetal (X National Meeting of the Spanish Society of Plant Physiology). Abstracts 193, p. 254. Pamplona, Spain, 1993. Poster and Oral Communication

6.Requerimiento de la luz azul para el transporte de aniones monovalentes en algas (Requirement of blue light for the transport of monovalent anions in algae). F.G. Witt and P.J. Aparicio. XVIII Congreso Nacional de Bioquímica. II Reunión Nacional del Grupo del Metabolismo del Nitrógeno (XVIII National Congress of Biochemistry. II National Meeting of the Nitrogen Metabolism Group). Abstracts, p. 38, GN. 6. San Sebastián, Spain, 1993. Oral Communication.

7.Caracterización de la incorporación de aniones monovalentes inducida por la luz azul en Monoraphidium braunii (Characterization of the uptake of monovalent anions induced by blue light in Monoraphidium braunii). F.G. Witt and P.J. Aparicio. V Simposium Nacional I Ibérico de Nutrición Mineral de las Plantas (V National and I Iberian Symposium of Plant Mineral Nutrition. In: Bases fisiológicas, bioquímicas y moleculares de la nutrición mineral de las plantas (Physiological, Biochemical and Molecular Basis of Plant Mineral Nutrition), p. 21. Badajoz, Spain, 1994. Conference.

8.El transporte de aniones monovalentes en Monoraphidium braunii, activado por un único fotorreceptor UV-azul (Transport of monovalent anions is activated by an unique Blue-UVA photoreceptor in Monoraphidium braunii). Giráldez, N., Witt, F.G., Quiñones, M.A. and Aparicio, P.J. XX Congreso Nacional de la Sociedad Española de Bioquímica y Biología Molecular (XX National Congress of the Spanish Society of Biochemistry and Molecular Biology). Madrid, Spain, 1997. Poster.

9.Stickstofferwerb bei Pflanzen: Biochemische Mechanismen zur Regulation der Nitratassimilation. Nitratreduktaseformen bei Peridinium gatunense (Dinophyceae) (Translation from German: Nitrogen uptake by plants: Biochemical mechanisms for the regulation of nitrate assimilation. Different forms of nitrate reductase in the dinoflagellate Peridinium gatunense). F.G. Witt, C. Stöhr and W.R. Ullrich. Sonderforschungsbereich 199: Molekulare Ökophysiologie der Pflanzen (Special area of investigation # 199: Plant Molecular Ecophysiology). Abstracts, pp 57-88. Darmstadt, Germany. 1998. Poster.

10.Descontaminación de aguas de usos agrícolas (Water remediation in agricultural lands). F.G. Witt. Buenas Prácticas Agrarias. Jornada de puertas abiertas de la Finca La Higueruela, CSIC (Good Agricultural Practices. Open Day in the Farm La Higueruela, Spanish Scientific Research Council). Santa Olalla, Toledo, Spain, 2002. Oral Communication

11.Descontaminación de aguas de usos agrícolas (Water remediation in agricultural lands). F.G. Witt. Buenas Prácticas Agrarias. Jornada de puertas abiertas de la Finca “La Higueruela”, CSIC (Good Agricultural Practices. Open Day in the Farm “La Higueruela”, Spanish Scientific Research Council). Santa Olalla, Toledo, Spain, 2003. Oral Communication

12.Contaminación de aguas. Sistemas biológicos para su recuperación (Water contamination. Biological systems for water reclamation). Federico Witt. Tiene futuro el secano en España? Una apuesta desde la Agricultura Ecológica. XI Jornadas técnicas de la Sociedad Española de Agricultura Ecológica (Does aridoculture have future in Spain? A bet from Ecological Agriculture. XI Technical Workshop of the Spanish Society of Ecological Agriculture). Santa Olalla, Toledo, Spain, 2005. Oral Communication

13.Singularidad tecnológica y transhumanismo: cuando la ciencia se encuentre con la ficción (Technological Singularity and Transhumanism: When Science Meets Fiction). Federico G. Witt. Semana de la Ciencia (Week of the Science). Círculo de Bellas Artes, Madrid, Spain, 2008. Conference

14.Singularidad Tecnológica (Technological Singularity). Federico G. Witt. Escuela de Ingeniería de la Universidad EAFIT. Medellín, Colombia. 2009. Seminar

PUBLICATIONS (only scientific publications on paper listed)

a) International:

1.-Aparicio P.J., Witt F.G., Ramírez J.M., Quiñones M.A. and Balandín T. Blue light induced pH changes associated with NO3-, NO2- and Cl- uptakes by the green alga Monoraphidium braunii. Plant, Cell and Environment 17, 1323-1330 (1994)

2.-Witt F.G. and Aparicio P.J. Effects of short pulses of blue light on the alkalinization associated with the uptakes of NO3- and Cl- by Monoraphidium braunii and related action spectra. Photochemistry and Photobiology 61, 619-626 (1995)

3.-Witt F.G. and Aparicio P.J. Characterization of the blue light-induced extracellular alkalinization associated with the monovalent anion uptake by Monoraphidium braunii. Competition between NO3- and Cl-. Physiologia Plantarum 94, 545-552 (1995)

4.-Witt F.G., Quiñones M.A. and Aparcio P.J. Photoregulation of the uptake of NO3-, NO2- and Cl- by the green alga Monoraphidium braunii. In: Plant Membrane Biology. (P. Brodelius, C-M. Larsson and I.M. Møller, Eds.), pp. 59-67. Oxford University Press (1996)

5.-Quiñones M.A., Giráldez N., Witt F.G. and Aparicio P.J. Blue light-dependent monovalent anion uptake. Physiologia Plantarum 100, 45-52 (1997)

6.-Ullrich W.R., Lazarová J., Ullrich C.I., Witt F.G. and Aparicio P.J. Nitrate uptake and extracellular alkalinization by the green alga Hydrodictyon reticulatum in blue and red light. Journal of Experimental Botany 49, 1157-1162 (1998)

7.-Witt F.G., Stöhr C. and Ullrich W.R. Soluble and membrane-associated nitrate reductases in the dinoflagellate Peridinium gatunense. The New Phytologist 142, 27-34 (1999)

8.-Mora C., Witt F.G., Aparicio P.J. and Quiñones M.A. Independent induction of two monovalent anion transport systems in the plasma membrane of Monoraphidium braunii. Journal of Experimental Botany 53, 1909-1918 (2002)

b) National (titles translated):

1.-Witt F.G. and Aparicio P.J. Characterization of the uptakes of NO3-, NO2- y Cl- by the green alga Monoraphidium braunii. In: Physiological, Biochemical and Molecular Basis of Plant Mineral Nutrition (C.M. Martínez Tinau, Ed.), pp. 21-29. Ediciones de la Consejería de Agricultura y Comercio de la Junta de Extremadura, Badajoz (1994)

2.-Aparicio P.J., Witt F.G., Ramírez J.M., Quiñones M.A. and Balandín T. Blue light induced uptake of NO3-, NO2- and Cl- by the green alga Monoraphidium braunii. In: Advances in Inorganic Nitrogen metabolism (Miguel G. Guerrero, Ed.), pp. 79-88. Publicaciones de la Universidad de Sevilla (1994)

3.-Witt, F.G. Photoregulation of monovalent anion uptake by the green alga Monoraphidium braunii. Action spectra of nitrate and chloride transport processes. 23 Boletín de la Sociedad Española de Fisiología Vegetal, pp.25-26, 1995

4.-Quiñones M.A., Giráldez N., Witt F.G. & Aparicio P.J. Blue light-dependent monovalent anion uptake in Monoraphidium braunii. In: Advances in Nitrogen Metabolism: From Physiology to Molecular Biology (J.M. Vega, P.J. Aparicio, F. Castillo and J.M. Maldonado, Eds.), pp. 387-402. Publicaciones de la Universidad de Sevilla (1998)

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