Dr Sudha Sahay - Resume

Dr. SUDHA SAHAY

PERSONAL PROFILE

Marital Status : Married

Date of Birth : 26.11.1968

Nationality : Indian

Permanent Residence Address : House No. E-104, Kaladeep, B/h Dhananjay

Tower, inside 100 feet Road, Satellite, Ahmedabad–380051, Gujarat, India

Residence Phone No : 079-********

Mobile No : 0-971*******

E-mail : **********@**********.***

FOREIGN LANGUAGES KNOWN

I. German

II. Italian

PROFESSIONAL/ACADEMIC EXPERIENCES IN INDIA/ABROAD

1. July 2009 –June 2011:

Designation: Sr. Scientist

Institute: Sardar Patel Renewable Energy Research Institute, Vallabh Vidaya Nagar, Dist. Anand, Gujarat, India

2. Dec. 2008 – March 2009:

Designation: Research Associate

Company: Concord Biotech Ltd, Trasad Road, Dholka, Dist. Ahmedabad, Gujarat, India

3. Dec. 2007 – June 2008:

Designation: Biology Content Writer

Company: Designmate (I) Pvt Ltd., Mithakali Road, Ahmedabad, Gujarat, India.

4. June 2005 – June 2006:

Designation: Senior Scientist

Company: SGS India Pvt. Ltd. , SG Highway, Ahmedabad, Gujarat, India.

5. Nov. 2004 – June 2005:

Designation: Lecturer

Department: Department of Biotechnology, St. Xavier’s Collage, Navrangpura,

Ahmedabad, Gujarat, India

6. June 1999 – Sept. 2001:

Designation: Post Doctoral Fellow

Funded by: DAAD, Germany

Department: Institute Of Plant Biochemistry, Halle, Germany

7. Sept. 1998 – Dec. 1998:

Designation: Senior Research Fellow

Funded by: Max Planck Institute of Microbiology, Marburg, Germany

Department: Max Planck Institute of Microbiology, Marburg, Germany.

8. 1995 – 1999:

Designation: Senior Research Fellow (DBT funded project)

Organization: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

COMMERCIAL AND ACADEMIC PROJECTS DEVELOPED

S. No. Project Title Year Name of the Industry / Institute

1 Screening and improving biomass production, lipid accumulation and oil recovery of microalgae from estuary region (Khambhat, Gujarat, India) by conventional and modern approaches 2011 Sardar Patel Renewable Energy Research Institute, Vallabh Vidaya Nagar, Dist. Anand, Gujarat, India

2 Improvement of Clostridium strains co-cultured with other bacterial strains for conversion of crop residues to ethanol. 2010 Sardar Patel Renewable Energy Research Institute, Vallabh Vidaya Nagar, Dist. Anand, Gujarat, India

3 Improvement in the storage conditions to increase the shelf life of Jatropha curcas seeds in terms of recovery and quality of its oil. 2009

Sardar Patel Renewable Energy Research Institute, Vallabh Vidaya Nagar, Dist. Anand, Gujarat, India

4 Optimize the in vitro conditions for the growth of estuary microalgae and their screening for oil/fat content 2009 Sardar Patel Renewable Energy Research Institute, Vallabh Vidaya Nagar, Dist. Anand, Gujarat, India

5 Strain Improvement of Zalerion arboricola (ATCC 74030) for the production of Pneumocandin B0.

2008 Concord Biotech Ltd

Trasad Road, Dholka,

Dist. Ahmedabad

Gujrat, India

6 Method established for GMO (Genetically modified organism/transgenic) testing of food grains viz. rice, wheat, barley, soybean, caster, pulses; processed agriculture products viz. food powder, lecithin and prawn feed 2005-2006

SGS India Pvt. Ltd.

SG Highway, Ahmedabad

Gujrat, India.

7 Differentiation of Basmati and Non-Basmati rice using SSR markers 2005-2006 SGS India Pvt. Ltd.

SG Highway, Ahmedabad

Gujrat, India.

8 Characterization of Piriformospora indica at molecular level using Subtraction Hybridization Approach 1999-2001 Institute Of Plant Biochemistry, Halle, Germany

9 Effect of mycorrhiza on Tissue Culture raised medicinal plants – Bacopa monniera and Artemesia annua 1995-1999 School of Life Sciences

Jawaharlal Nehru University,

New Delhi

10 Active translocation of phosphate (P32) in rice and carrot (root organ culture) by the Piriformospora indica.

1998 Max Planck Institute of Microbiology,

Marburg, Germany.

AWARDS OBTAINED

• Qualified in Graduate Aptitude Test in Engineering (GATE) (1996)

• Awarded Senior Research Fellow in DBT funded Project (1995 to 1999)

• Awarded DAAD - Post Doctoral Fellow, Germany (1999 to 2001)

EDUCATIONAL QUALIFICATIONS

• Post Doctorate (1999-2001) from Institute of Plant Biochemistry, Halle,

Germany

Project Title: Characterization of fungus - Piriformospora indica at molecular level

• Ph. D. (1995 –1999) from School of Life Sciences, Jawaharlal Nehru University, New Delhi and Department of Biotechnology, Hamdard University, Hamdard Nagar, New Delhi, India (Joint Registration)

Project Title: Effect of mycorrhiza on tissue culture raised medicinal plants Bacopa monneria and Artemesia annua

• M. Phil. (1993 - 1994) from Department of Botany, Delhi University, New Delhi, India

Project Title: Bio-control of weed: Euphorbia geniqulata highly grown in wheat

field.

• M. Sc. (1990-1993) from Department of Botany, Delhi University, New Delhi, India

Specialization: (i) Microbiology & Plant Pathology

(ii) Advanced Ecology

• B. Sc. (1987 -1990) from Miranda House College, Delhi University, New Delhi, India.

• XIIth (1986) in Science from Delhi Senior Secondary School Examination (CBSE Board), New Delhi, India.

• Xth (1984) from Delhi Secondary School Examination (CBSE Board), New Delhi, India.

PUBLICATIONS

1. Hindav R, Kumari M, Mondal N, Paul J, Sahay NS, Sharma J, Singh A, Sudha and Varma A (1998) One kilo tropical soil = one kilo gold : this is microbial science. In: Microbes for Health, Wealth and Sustainable Environment (ed., Varma A), MPH, New Delhi, pp 1-23.

2. Sahay NS, Sudha, Singh A and Varma A (1998) Treands in endomycorrhizal research. Indian Journal of Experimental Biology, NISCOM 36: 1069-1086

3. Roy A, Sahay NS, Sudha, Singh A and Varma A (1998) Concept in myco- and photosymbionts interactions - a review. The Botanica 48: 107-115

4. Sudha, Hurek T and Varma A (1998) Active translocation of phosphate (P32) to rice and carrot by deuteromycetes Piriformospora indica. In: Second International Conference on Mycorrhiza, July 5-9, Sweden, pp. 162.

5. Sudha, Hurek T, and Varma A (1998) Translocation of phosphorus in a cultivable root colonizing fungus Piriformospora indica. ISME - 8, Halifax, Canada, pp. 316.

6. Verma S, Sahay NS, Sudha, Sharma J, Hindav R, Singh A, Meera and Varma A (1998) A novel plant promoting root fungus. In: International symposium on Trends in Life Science. December 17-19, JNU, New Delhi, pp. 54.

7. Sahay NS, Sudha and Varma A (1998) Piriformospora indica, a new symbiotic fungus. In: National symposium on management of waste land to protect environment - retrospect & prospect. At Bhagalpur University (Bihar), April 24-25, pp. 20.

8. Sudha, Sahay NS and Varma A (1998) Piriformospora indica, a new symbiotic fungus. In: National seminar on integrated management of plant resources. Chindwara (MP), January 23-24, pp. 48.

9. Varma A, Sudha, Sahay NS, Butehorn, B and Franken P. (1999) Piriformospora indica, a cultivable plant growth promoting root endophyte with similarities to arbuscualr mycorrhizal fungi. Applied and Environment Microbiology 65: 2741-2744.

10. Sudha, Kumar S, Kaur H, Narula A, Srivastava P S and Varma A (1999) Mycorrhiza aided biological hardening of Micropropagated plantlets. In: Advances in Microbial Biotechnology (eds. Tewari L, Singh G and Chamola), APH Publication Corporation, New Delhi, pp. 469-484.

11. Varma A , Sudha, Sahay NS, Singh A, Kumari M, Bharati K, Sarbhoy A K, Walter M H, Maier W, Strack D and Franken P (1999) Abatement through biological treatment of industrial effluents. Pub. Central Pollution Control Board, New Delhi, pp. 1-23

12. Varma A , Rai MK, Sudha and Sahay N (2000) Microbial-Biotechnology: New-Paradigms and role in sustainable Agriculture. In: Microbial biotechnology for sustainable development and productivity (ed Rajak RC). Scientific Publishers ( India), pp. 22-37

13. Varma A , Singh A, Sudha, Sahay N, Sharma J, Roy A, Kumari M, Rana D, Thakran S, Deka D, Bharti K, Franken P, Hurek T, Blechert O, Rexer K-H, Kost G, Hahn A, Hock B, Maier W, Walter M and Strack D, Kranner I (2001) Piriformospora indica: A cultivable mycorrhiza-like endosymbiotic fungus. In: Mycota IX, Eds. Esser, K. and Hock B, Springer Series, Germany, pp. 123-150

14. Varma A, Singh A, Sudha, Sahay N S, Kumari M, Bharati K, Sarbhoy AK, Maier W, Walter MH, Strack D, Franken P, Singh An, Malla R and Hurek T (2001) Piriformospora indica: A plant stimulator and pathogen inhibitor arbuscular mycorrhiza-like fungus. In: Microorganisms in Bioremediation, (eds) DK Markandey and NR Markandey, Capital Book Company, New-Delhi, India, pp. 71-89.

THEME OF POST DOC WORK DONE AT IPB, HALLE, GERMANY

(1999-2001)

cDNA clones was isolated as a result of subtraction hybridization to identify genes showing differential expression during the interaction between Zea mays and Piriformospora indica.

Methodology:

Total RNA from the P. indica colonized and non colonized (control) maize root was isolated. Poly A+ mRNA was purified from total RNA and followed by cDNA construction. The cDNA from control and treated plant roots were subjected to subtraction hybridization to obtain subtracted cDNA. The final subtracted PCR product were directly plated on LB-Ampicilline medium infested with X-gal and IPTG and incubated for overnight at 370C. About 1000s of blue and white colonies were formed. The selected white colonies were further followed by fast screening test to check the presence of insert. Plasmid DNA of more than 500 transformed clones were individually isolated and sequenced. All sequences were searched in gene bank to find their homology / identity. Out of these 17 interesting sequences were found.

Most important among them which were showing remarkable level of differential expression were of Calcium dependent protein kinase (CDPK) which was showing maximum homology with Oryza sativa CDPK, another was phosphate transporter gene and genes (VP14) involved in the enzymatic cleavage of carotenoid.

Northern blots were performed of total RNA with alpha P32 labeled CDPK-clone probe.

cDNA library screening:

Maize cDNA library was obtained from Dr. M.H.Walter (IPB, Halle, Germany). In cDNA library the information encoded by the maize RNA is converted in to stable DNA duplex or complementary DNA (cDNA) and then inserted in to a self replicating lambda Uni-Zap XR Vector. These ligated cDNA was inserted in to the XL1-Blue MRF’ cells. Once the information available in the form of a cDNA library, individual processed segments of the original genetic information can be isolated and examined with relative ease. By doing titration, accurate number of cells was checked and calculated number of phages were mixed with N2V medium and plated on LB medium supplemented with maltose, MgSo4 and tetracycline. Plates were incubated at 370C for 8 hrs to grow the cells. Many colonies were observed. By using filter membrane, the colonies were picked up, washed with buffer and hybridized with alpha P32 labeled CDPK DNA for overnight at 600C. After autoradiography 10 colonies/signals were observed. These colonies were further screened by same process and finally got two colonies/signals. These DNA were full length sequenced and searched in the gene bank by using NCBI-Blast and Husar Programme. These were named as CDPK1 and CDPK2. Both these CDPKs were made aligned with other CDPKs reported from the plants and analyzed them.

In the Northern blot analysis the total RNA from control roots gave strong signal as compared to treated, after when hybridized with alpha P32 labeled CDPK – clone probe. To reconfirm or ensure the results the phosphor-imager estimation test was done, with this the exact amount of signal (after Northern hybridization) present in each lane was obtained. The result also confirms the strong signal of CDPK in control as compare to treated root.

Southern hybridization:

Genomic DNA from maize leaves was isolated. The DNA was digested with many different restriction enzymes, randomly selected and runed over the gel. Different intensity bands were obtained. These bands were transferred on membrane and hybridized with alpha P32 labeled CDPK clone probe to check, whether the clone is a single segment or many segments.

Semi-Quantitative RT-PCR:

The use of PCR to amplify and quantify mRNA content allows analysis of gene expression. By using a common approach, the RT-PCR product from the expressed gene be compared with the RT-PCR product from a reference gene mRNA internal standard. Here I used Ubiquitin as an internal standard in a parallel series of reaction. The different amount of RT product performed PCR by using ubiquitin primers and then run over the gel (total 6 concentrations were used). The bands were obtained in a regular gradation. Then control and treated root RNA undergo RT PCR by using CDPK primers and compare the band intensity with standards to check the difference in treated and control CDPK expression.

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