Project Development

Ying Qu

San Diego, California, 858-***-****, ********@*****.***,

http://www.linkedin.com/in/yingqu

Bioanalytical Mass Spectrometry

Amylin Pharmceuticals, Inc., San Diego (Investigator) 2009-2012

• Supported a variety of projects in discovery and development of biological therapeutics (peptide and protein drugs) by providing preclinical and clinical pharmacokinetic and toxicokinetic profiles.

• Supported early stage peptide and protein discovery by providing high throughput in vitro screening assay to predict in vivo stability.

• Developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for identification and quantification of peptide (protein) drugs and metabolites, as well as endogenous protein biomarkers in different biological matrices by following FDA guidance.

• Comparison of data profile which obtained by using LC-MS/MS with ELISA.

• Evaluated immunogenicity of therapeutic protein by measuring ADA bound drug and free drug in human plasma.

• Wrote research protocols and SOPs; presented data to senior leadership and other scientists with different backgrounds; documented research reports; submitted abstracts and posters to scientific conferences.

• Managed laboratory; Maintained and troubleshooted multiple online solid phase extraction (SPE) and LC-MS/MS instruments, and automatic sample processor.

1University of California San Diego (Postdoctoral fellow ) 2007-2009

2The Scripps Research Institute, San Diego (Guest Investigator) 2007-2009

• Set up in vivo microdialysis laboratories;

• Measuring Neurotransmitters in rat brain microdialysate by using capillary electrophoresis (CE) - laser induced fluorescence detection (LIF).

• Measuring Neuropeptide in rat brain microdialysate by using Radioimmunoassay (RIA).

Neuroscience Drug Discovery, Johnson & Johnson Pharmaceutical Research

& Development, L.L.C. San Diego, (Postdoctoral Fellow) 2003-2006

• Developed and validated different analytical methods, such as LC-MS/MS and high performance liquid chromatography-electrochemical detector (HPLC-ED), and CE-LIF to measure the biomarkers and free drugs in rat brain. Supported lead optimization of small molecules by evaluating efficacy (PD) and BBB penetration.

• Characterized drug candidate and its metabolites by comparing the pharmacokinetic-pharmacodynamic (PK/PD) profile;

• Recommended, evaluated, and implemented new analytical technology/instrumentation to improve drug discovery technical direction. Initiated a collaborative project within the company across different departments and with scientists outside of the company.

National Institutes of Health, Bethesda. (Postdoctoral Fellow) 1999-2003

• Initiated in vivo imaging method of brain serotonergic neurotransmission involving phospholipase A activation and arachidonic acid release in unanesthetized rats.

Catholic University of Leuven, Belgium (Free researcher, Ph.D. Study) 1993-1999

• Supported neuroscience research as an Analytical Chemist, by developing methods of HPLC, CE, ELISA and electrochemical enzyme immunoassay;

EDUCATION

Certificate ADMET Process University of California, San Diego USA

Ph.D. Science Catholic University of Leuven Belgium

MS/BS Analytical Chemistry Lanzhou University China

Professional Addendum

BOOK CHAPTERS

1. Ying Qu, Loren Olson, Xiaohiu Jiang, Leah Aluisio, Christopher King, Elliott B. Jones, Timothy W. Lovenberg, (2011) Evaluating PK/PD Relationship of CNS Drug by Using Liquid Chromatography/Tandem Mass Spectrometry Coupled With In Vivo Microdialysis. In: Applications of Tandem Mass Spectrometry. ISBN 978-953-307-870-0, Editor: Dr. Jeevan Prasain, InTech.

SELECTED PUBLICATIONS

1. Qu Y, Bellows C, Ahn J, Taylor S, Burkey J, Wang Y, Identification and quantification of Davalintide (AC2307), a novel Amylin-mimetic peptide, and its metabolite in animal plasma: LC-MS/MS versus ELISA. Analytical and Bioanalytical Chemistry 2012 (Submitted).

2. Qu Y, Aluisio L, Lord B, Boggs JD, Mazur C, Lovenberg TW. Pharmacokinetics and pharmacodynamics of norfluoxetine in rats: increasing extracellular serotonin level in frontal cortex Pharmacol Biochem Behav. 2009 92: 469-473.

3. Barbier AJ, Aluisio L, Lord B, Qu Y, Wilson SJ, Boggs JD, Bonaventure P, Miller K, Fraser I, Dvorak L, Pudiak C, Dugovic C, Shelton J, Mazur C, Letavic MA, Carruthers NI, Lovenberg TW. Pharmacological characterization of JNJ-28583867, a histamine H(3) receptor antagonist and serotonin reuptake inhibitor. Eur J Pharmacol. 2007 576(1-3):43-54.

4. Qu Y, Li Y, Vandenbussche E, Vandesande F, Arckens L. In vivo microdialysis in the visual cortex of awake cat. II: sample analysis by microbore HPLC-electrochemical detection and capillary electrophoresis-laser-induced fluorescence detection. Brain Res Brain Res Protoc. 2001 7(1):45-51.

5. Li YM, Qu Y, Vandenbussche E, Arckens L, Vandesande F. Analysis of extracellular gamma-aminobutyric acid, glutamate and aspartate in cat visual cortex by in vivo microdialysis and capillary electrophoresis-laser induced fluorescence detection. J Neurosci Methods. 2001 105(2):211-5.

6. Qu Y, Berghman LR, Vandesande F. An electrochemical enzyme immunoassay for chicken luteinizing hormone: extension of the detection limit by adequate control of the nonspecific adsorption. Anal Biochem. 1998 259(2):167-75.

7. Qu Y, Arckens L, Vandenbussche E, Geeraerts S, Vandesande F. Simultaneous determination of total and extracellular concentrations of the amino acid neurotransmitters in cat visual cortex by microbore liquid chromatography and electrochemical detection. J Chromatogr A. 1998 798(1-2):19-26.

8. Qu Y, Moons L, Vandesande F. Determination of serotonin, catecholamines and their metabolites by direct injection of supernatants from chicken brain tissue homogenate using liquid chromatography with electrochemical detection. J Chromatogr B Biomed Sci Appl. 1997 704(1-2):351-8.

9. Qu Y, Vandesande F, Arckens L. Identification and quantification of monoaminergic neuromodulators in the sub-cortical region of cat visual cortex by microbore HPLC-ED and protein assay, J. Liq. Chrom. & Rel. Technol.,2001 24(14), 2087-2100.

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