Junxia Lu, PhD

Cell Phone: 571-***-****

Email: lujunxia@ yahoo.com

Education

*/****-*/**** **. D. in Biochemistry Miami University, Oxford, Ohio

Advisor: Gary Lorigan

Dissertation: Structural and dynamic properties of phospholipid model membranes (in gel and liquid-crystalline phases) and membrane-associated macromolecules using solid-state NMR

8/1999-6/2002 M.S. in Inorganic Chemistry Fudan University, Shanghai, China

8/1995-6/1999 B.S. in Chemistry Fudan University, Shanghai, China

Research experience

2012 Postdoctoral research associate at Pacific Northwest National Laboratory

• Study the structure of amelogenins (present and play a significant role in teeth enamel formation) and the interactions with hydroxyapatite using solid-state NMR dipolar recoupling techniques

2007-2011 Postdoctoral fellow at NIDDK, National Institute of Health

Advisor: Robert Tycko, Ph.D.

• The structure of amyloid fibrils from human brain with late-onset Alzheimer's disease: extract amyloid fibrils brain senile plaques; study the fibril conformation using multidimensional solid-state NMR 2D techniques (15N-13Cα correlation, 15N-13CO correlation, PITHIRD, REDOR or TEDOR type experiments) and transmission electron microscope (TEM)

• The structure and dynamics of amyloid peptide precursor protein transmembrane domain in different memebrane environment using Solid-state NMR 2D 13C-13C,15N-13C and relaxation study

• Study the oligomeric state of integral membrane protein vpu found in HIV-1 utilizing solid-state NMR, photo-crosslinking of protein, gel-electrophoresis, MALDI mass spectroscopy, LC/MS, HPLC/FPLC and molecular modeling

• Design and test the different strategies to clone, express and purify isotopically labeled HIV-1 vpu protein in bacteria, maximize the yield and stability of membrane protein for structural study

2002-2007 Ph. D. research at Miami University (Advisor: Gary Lorigan, Ph.D.)

• Study the structural and dynamic properties of phospholipid membranes in gel phase and liquid-crystalline phase (bicelles and bilayers) using 2H, 31P NMR and EPR

• Structural study of human phospholamban and antimicrobial memebrane peptides utilizing 15N and 13C NMR on aligned liquid-crystalline membranes (magnetically aligned bicelles or mechanically aligned bilayers on glass plates).

• Expression and purification of membrane protein phospholamban (a transmembrane protein involved in regulating heart beats) combining affinity chromatograph and reverse-phase chromatograph (HPLC/FPLC)

1999-2002 Master and bachelor research at Fudan University, China

• Construction of metalloprotein cytochrome b5 mutants expression vector (a protein involved in electron transportation in the mitochondrion)

• Expression, purification and function study of cytochrome b5 using UV-Vis, CD, stopped flow spectroscopic techniques

NMR spectroscopy experience

• Perform Nitrogen and Helium refills

• Optimize probes and instrument performance regularly

• Regularly modify and write pulse-sequences for solid-state NMR

• Assist regular users at Miami University (1H,13C NMR, COSY, HSQC, NOSEY, temperature variation and relaxation studies etc.)

Award

2006-2007 Doctoral Dissertation Scholarship Award at Miami University (only one student got

awarded each year in the department)

2006 Graduate Achievement Award of the Graduate School, Miami University

2005 47th Rocky Mountain Conference in Analytic Chemistry

- Solid-state NMR section Travel Award

2004 William H. Charch Scholarship at Miami University

2003 William H. Charch Scholarship at Miami University

Publications

1. Lu, J.X., Sharpe S., Yau, W.Y. and Tycko R. Oligomerization State and Supramolecular Structure of the HIV-1 Vpu Protein Transmembrane Segment in Phospholipid Bilayers Protein Science (2010) 19, 1877-1896

2. Lu, J.X., Yau, W.Y. and Tycko R. Evidence from solid state NMR for non-helical conformations in the transmembrane domain of the amyloid precursor protein Biophysical Journal (2010) 100, 711-719

3. Abu-Baker, S., Lu, J.X., Chu, S.D., Shetty, K.K., Gor”kov, P.L., and Lorigan, G.A. The structural topology of wild-type phospholamban in oriented lipid bilayers using N-15 solid-state NMR spectroscopy Protein Science (2007) 16, 2345-2349.

4. Abu-Baker, S., Lu, J.X., Chu, S.D., Brinn, C.C., Makaroff, C.A., and Lorigan, G.A. Side chain and backbone dynamics of phospholamban in phospholipid bilayers utilizing H-2 and N-15 solid-state NMR spectroscopy Biochemsitry (2007) 46, 116**-*****.

5. Lu, J.X., Blazyk J. and Lorigan, G. A. Exploring membrane selectivity of the antimicrobial peptide KIGAKI using Solid-State NMR spectroscopy Biochimica et biophysica Acta (2006) 1758, 1303-1313.

6. Lu, J.X., Damodaran, K., and Lorigan, G. A., “Probing Membrane Topology by 1H-13C Heteronuclear Dipolar Solid-State NMR Spectroscopy” J. Magn. Reson. (2006) 178, 283-287.

7. Lu, J.X., Damodaran, K., Blazyk, J., and Lorigan, G. A. “Solid-State NMR Relaxation Studies of the Interaction Mechanism of Antimicrobial Peptides with Phospholipid Bilayer Membranes” Biochemistry (2005) 44, 102**-*****.

8. Lu, J.X., Caporini, M. A., and Lorigan, G. A. “The Effects of Cholesterol on Magnetically Aligned Phospholipid Bilayers: A Solid-State NMR and EPR Spectroscopy Study” J. Magn. Reson. (2004) 168: 18-30.

9. Li, S., Lu, J.X., Gan, J. H., Wang, Y. H., Huang Z. X. and Xia Z. X. Structure of the F58W mutant of cytochrome b5: the mutation leads to multiple conformations and weakens stacking interactions Acta Crystallographica, Section D: Biological Crystallography (2005) D61(2), 180-189.

10. Su, H., Lu, J.X., Wang, Y. H., Ren, Y., Xie, Y. and Huang Z. X. Study on the DME-cytochrome b(5) and its mutants at site of F58 Chinese Science Bulletin (2004) 49, 18, 1914-1919

11. Wang, W. H., Lu, J.X., Yao P., Xie, Y. and Huang Z. X. The distinct heme coordination environments and heme-binding stabilities of His39Ser and His39Cys mutants of cytochrome b(5) Protein Engineering (2003) 16,12 1047-1054

12. Wang, Y. H., Lu, J.X., Wang, W. H., Ren, Y., Xie, Y. and Huang Z. X. Roles of Phe58 in Stabilizing Structure of Cytochrome b5 Chinese Science Bulletin (2002) 47, 24, 2063-2066.

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