Biochemistry,Molecular Biology,Cell Culture,AssayDevelopment Scientist

OBJECTIVE

I am looking for a research position where I can effectively apply creativity and hands-on experiences to the development of new therapeutics.

QUALIFICATIONS

PhD in biochemistry and molecular biology, MS in Biochemistry

4+ years of hands-on experience on metabolic disease, cancer therapy, and neuroscience.

Superior experience on DNA cloning, RNA interference, adenovirus/retrovirus, cell culture, animal models, hybridoma, monoclonal antibody, transcriptional regulation and functional genomics, biostatistics, and computer analysis

Experience with leading research projects and mentoring technicians, graduate students, and postdoc fellows.

SKILLS

• Extensive research experiences on metabolic disease, cancer therapeutics, signal transduction and neurobiology

• Molecular biology --- DNA cloning, PCR/qPCR, RNAi (siRNA and shRNA), adenoviral and retroviral techniques, transcriptional regulation, functional genomics

• Protein and immunol bioassays --- protein engineering, protein purification and characterization, HPLC, SDS-PAGE; ELISA, immunoblot, immunoprecipitation, immunohistochemistry, monoclonal antibody, low molecular weight therapeutical peptide and protein

• Cell culture --- mammalian cell lines, primary cell culture, hybridoma, cell-based assay development

• Animal-related experience --- animal surgery, transgenic and knockout mice developing, genotyping and colony maintenance, tissue sampling, tissue-based bioassay development, diabetes and metabolism in vivo model development, in vivo metabolic analysis (MRI, metabolic chamber, GTT, ITT, etc)

• Computer analysis --- familiar with online tools and major analysis suites: NCBI, EBI, ExPASy, UCSC, SDSC, etc.; promoter analysis, phylogenetic footprinting; biostatistics; Unix/Linux

RESEARCH EXPERIENCE

Tufts University, Boston, MA

October 2007 - Present Scientist Metabolic diseases

• Analyzed metabolic consequences in lipid droplet protein deficient animals

• Established new perilipin transgenic strain

• High-throughput identified perilipin-associated proteins by immunoprecipitation and mass spec analysis

• In silico explored potential perilipin phosphorylation sites by bioinformatics analysis

Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA

January 2003 – October 2007 Research Fellow Metabolic diseases

• Discovered lipocalin2 as new cytokine in adipocytes that plays a role in obesity-induced insulin resistance

• Elucidated a set of transcriptional factors involved in adipocyte differentiation by qPCR-based DHSA high throughput screening

• In vivo analyzed adipose-selective regulatory motifs by transgenic studies

University of Iowa, Iowa City, IA

September 2001 – January 2003 Postdoctoral Research Scholar Endocrinology

• Transcription and hCG binding of LH receptors was dramatically induced by FSH or 8-Br-cAMP in primary cultured granulosa cells from DES-primed immature rats

• Regulation of LH receptor by FSH/cAMP in mammalian granulosa cell line KK-1 in similar yet distinguishable manner

National Cancer Institute, National Institute of Health, Bethesda, MD

August 1999 – September 2001 Visiting Scholar Cancer biology

• Trans-regulation of ABCG2 gene during the course of drug selection in human S1 and MCF-7 cells

• Mutation of a single amino acid residue in ABCG2 protein significantly changes substrate specificity, thus altering the drug resistance phenotype

• Transcriptional factor MZF1 possesses a repressively regulatory function in ERCC1 expression

EDUCATION

Ph.D. in Biochemistry and Molecular Biology September 1995 – August 1998

Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China

Ph.D. Dissertation: Signal Transductions Evoked by Neuropeptide ZNC(C)PR (AVP(4-8)) in Rat Brain and in Neuroglioma Cells.

M.S. in Biochemistry September 1992 – July 1995

East China Normal University, Shanghai, China

(Thesis work conducted in SIBCB, Chinese Academy of Sciences)

M.S. Thesis: Purification of a Ribosome Inactivating Protein from Luff Cylindrica Seeds and its Application in mAb-targeted Cancer Therapy.

B.S. in Biology September 1986 – July 1990

East China Normal University, Shanghai, China

B.S. Thesis: Histological Studies on Salanx ariakensis Brain Structure – a Potential Teaching Model for Fish Brain.

PROFESSIONAL SOCIETY

American Society for Biochemistry and Molecular Biology, American Diabetes Association, The Endocrine Society, Sigma Xi

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