Archana Dilip

**** **** ******** ******, ***# *13, Milwaukee, Wisconsin-53227

302-***-****

dlec0u@r.postjobfree.com

Objective

To obtain a research based position in a laboratory that will provide the right platform to learn new skills

Education

The University of Texas at Dallas, Richardson, Texas

Master of Science in Cell and Molecular Biology, Dec. 2010

Overall GPA: 3.4

Anna University, Chennai, India

Bachelor of Technology in Bio-technology, May 2008

Overall percentage: 88% - Gold medalist and State rank holder

Research Experiences

Research Technologist, Medical College of Wisconsin, Milwaukee, Aug 2011- current date

Project: Studying the effect of Mitochondrial Targeted antioxidants on Hepatocellular carcinoma

Research Assistant, University of Texas Southwestern Medical Center at Dallas, Texas

Department of Advanced Imaging Research center, Jan 2010 – Aug 2011

Project: Developing reconstituted Low density lipoproteins with Docosahexaenoic Acid (DHA - an omega 3 fatty acid) for use as a therapeutic agent for Hepatocellular cancer

Experiments conducted

• Isolated lipoproteins from human plasma, characterizing the Low density lipoproteins by biochemical assays

• Reconstituted Low Density Lipoproteins with Omega-3 fatty acids

• Cultured primary hepatocytes and hepatic carcinoma cell lines

• Conducted cell proliferation assays in tumor and normal hepatic cell lines using the LDL-DHA reconstituted molecule

• Developed orthotropic tumor models in mice and rats to evaluate the invivo effects of LDL-DHA

• Conducted biodistibution studies in mouse and LDL receptor expression/binding studies

• Performed Confocal microscopy based assays to study omega-3 fatty acid induced lipid peroxidation in cells and tissues

Research Intern, Central Leather Research Institute (CLRI), India, Dec 2007 – April 2008

Project: Phytoremediation of Chromium from tannery effluent polluted lands

• Identified two plant species namely Brassica Juncea and Glycine Max Merr capable of enhanced uptake and translocation of chromium from tannery effluent polluted lands

• Studied the effect of chelators on enhancement of plant chromium uptake

• Performed spectrophotometric analysis of chromium content in plant roots and shoots following growth in high chromium containing soil

• Conducted field trials in different parts of South India

Research Volunteer, Tuberculosis Research Center (TRC), India, May 2007- Aug 2007

• Performed ELISA assays, Immunoassays

• Performed other basic laboratory techniques and learnt to operate basic lab instruments like spectrophotometer, fluorometer and calorimeter.

• Ordered Lab supplies and Maintained records

Teaching experience

Teaching Assistant (Microbiology), July 2009-Jan 2010

• Set up laboratory experiments and performed basic microbiology techniques

• Mentored students on microbiology lab techniques

• Assisted the professor in conducting classes and evaluating exams

Technical skills

• Experienced in reverse phase and size exclusion chromatography techniques - operating HPLC, FPLC (UNICORN software), UV Spectrophotometer, Fluorometer and CD spectrometer (protein and lipoprotein studies), dynamic light scatterer

• Cell culture techniques – cultured (mouse, rat and human) normal and tumor hepatic and pancreatic cancer cell lines(TiB73, TiB75, Huh7, HepG2, Hep3B, H42E, Bxpc, Aspc, Panc1)

• Confocal Microscopy

• Rodent handling techniques –Tail vein injections, developed hepatic tumor models, mouse autopsy and organ collection

• Proficient in Gel electrophoresis, western blotting, nucleic acid extraction, Cell viability assays, Biochemical assays (Phospholipid, Cholesterol, protein, triglyceride, T-bar etc.)

• Lipoprotein isolation from human plasma by density gradient ultracentrifugation and lipoprotein reconstitution

• Handling human tissues and bio-fluids

Strengths and Achievements

• Pleasant demeanor with good interpersonal skills

• Possess excellent communication skills

• Can design and implement experiments independently and analyze scientific results

• A self- motivated, dedicated individual with the ability to perform efficiently in a team

• A competent learner with the capacity to grasp new techniques instantly

• Excellent presentation and organization skills

Achievements

• Excellent Academic records - State Rank holder in Undergraduate Biotechnology program

• Trained for 10 yrs in south Indian classical music. Have given several stage performances

• Volunteer in Banyan Asha – An organization for destitute women

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