Cell biologist - 2 yrs experience
Resume:
Archana Dilip
**** **** ******** ******, ***# *13, Milwaukee, Wisconsin-53227
*******.******@*****.***
Objective
To obtain a research based position in a laboratory that will provide the right platform to learn new skills
Education
The University of Texas at Dallas, Richardson, Texas
Master of Science in Cell and Molecular Biology, Dec. 2010
Overall GPA: 3.4
Anna University, Chennai, India
Bachelor of Technology in Bio-technology, May 2008
Overall percentage: 88% - Gold medalist and State rank holder
Research Experiences
Research Technologist, Medical College of Wisconsin, Milwaukee, Aug 2011- current date
Project: Studying the effect of Mitochondrial Targeted antioxidants on Hepatocellular carcinoma
Research Assistant, University of Texas Southwestern Medical Center at Dallas, Texas
Department of Advanced Imaging Research center, Jan 2010 – Aug 2011
Project: Developing reconstituted Low density lipoproteins with Docosahexaenoic Acid (DHA - an omega 3 fatty acid) for use as a therapeutic agent for Hepatocellular cancer
Experiments conducted
• Isolated lipoproteins from human plasma, characterizing the Low density lipoproteins by biochemical assays
• Reconstituted Low Density Lipoproteins with Omega-3 fatty acids
• Cultured primary hepatocytes and hepatic carcinoma cell lines
• Conducted cell proliferation assays in tumor and normal hepatic cell lines using the LDL-DHA reconstituted molecule
• Developed orthotropic tumor models in mice and rats to evaluate the invivo effects of LDL-DHA
• Conducted biodistibution studies in mouse and LDL receptor expression/binding studies
• Performed Confocal microscopy based assays to study omega-3 fatty acid induced lipid peroxidation in cells and tissues
Research Intern, Central Leather Research Institute (CLRI), India, Dec 2007 – April 2008
Project: Phytoremediation of Chromium from tannery effluent polluted lands
• Identified two plant species namely Brassica Juncea and Glycine Max Merr capable of enhanced uptake and translocation of chromium from tannery effluent polluted lands
• Studied the effect of chelators on enhancement of plant chromium uptake
• Performed spectrophotometric analysis of chromium content in plant roots and shoots following growth in high chromium containing soil
• Conducted field trials in different parts of South India
Research Volunteer, Tuberculosis Research Center (TRC), India, May 2007- Aug 2007
• Performed ELISA assays, Immunoassays
• Performed other basic laboratory techniques and learnt to operate basic lab instruments like spectrophotometer, fluorometer and calorimeter.
• Ordered Lab supplies and Maintained records
Teaching experience
Teaching Assistant (Microbiology), July 2009-Jan 2010
• Set up laboratory experiments and performed basic microbiology techniques
• Mentored students on microbiology lab techniques
• Assisted the professor in conducting classes and evaluating exams
Technical skills
• Experienced in reverse phase and size exclusion chromatography techniques - operating HPLC, FPLC (UNICORN software), UV Spectrophotometer, Fluorometer and CD spectrometer (protein and lipoprotein studies), dynamic light scatterer
• Cell culture techniques – cultured (mouse, rat and human) normal and tumor hepatic and pancreatic cancer cell lines(TiB73, TiB75, Huh7, HepG2, Hep3B, H42E, Bxpc, Aspc, Panc1)
• Confocal Microscopy
• Rodent handling techniques –Tail vein injections, developed hepatic tumor models, mouse autopsy and organ collection
• Proficient in Gel electrophoresis, western blotting, nucleic acid extraction, Cell viability assays, Biochemical assays (Phospholipid, Cholesterol, protein, triglyceride, T-bar etc.)
• Lipoprotein isolation from human plasma by density gradient ultracentrifugation and lipoprotein reconstitution
• Handling human tissues and bio-fluids
Strengths and Achievements
• Pleasant demeanor with good interpersonal skills
• Possess excellent communication skills
• Can design and implement experiments independently and analyze scientific results
• A self- motivated, dedicated individual with the ability to perform efficiently in a team
• A competent learner with the capacity to grasp new techniques instantly
• Excellent presentation and organization skills
Achievements
• Excellent Academic records - State Rank holder in Undergraduate Biotechnology program
• Trained for 10 yrs in south Indian classical music. Have given several stage performances
• Volunteer in Banyan Asha – An organization for destitute women
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