Biographical Sketch

TAPAN KUMAR MONDAL

*** ***** ***** ****

Valatie, New York-12184

E-mail: d12bqg@r.postjobfree.com

Phone #518-***-**** Fax: 518-***-****

Present Position Title Research Associate

NYS Department of Health

Wadsworth Center, Department of Infectious Disease & Host Defense,

Albany, New York-12201

OBJECTIVE

An Enthusiastic and dedicated researcher, with experience in a wide range of cutting-edge laboratory technology (Cell Biology, Immunology, Neurology, Proteomics, Pathology etc.) seeking a Research position

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EDUCATION / TRAINING

INSTITUTION AND LOCATION DEGREE/ DIPLOMA

YEAR FIELD OF STUDY

Utkal University, Bhubaneswar, Orissa, India Ph.D. 2006 Cancer ( Molecular and Cellular) Biology

Institution of Chemists (India), WB, Calcutta, India

M.Sc. (AIC)

1996 Analytical Chemistry

Jadavpur University, Calcutta, WB, India

Medical Laboratory Technology 1990 Clinical Pathology, Histopathology and Biochemistry

PROFESSIONAL TRAINING

05/1985 - 06/1996 Department of Pathology, S.S.K.M. Hospital, Calcutta University, Calcutta,

Advanced training on experimental Hematology Paracytology, Serology and

Bacteriology. Advanced training on diagnosis of pathology

07/1995 - 11/1996 Department of Pathology, S.S.K.M.Hospital and IPGMR&E,

Advanced training on histopathology and cellular image analysis

04/1990 - 07/1990 Department of Biochemistry & Molecular Biology, Bose Institute, Calcutta

Advanced training for Molecular Biology, Immunology and Clinical Biochemistry

Advanced training on immunohistochemistry and cellular image analysis

WORK EXPERIENCES

1987-1992 Research Assistant. Indian Council of Medical Research program

Department of Clinical Pathology (Hematology, Bacteriology, Serology) and Clinical

Biochemistry, Indian Post Graduate Medical Education & Research

(IPGME&R), SSKM Hospital, Calcutta, India.

1993-2000 Research Assistant/Graduate Student (Ph D), Department molecular genetics and

Immunotechnology Section, Bose Institute, Calcutta, India & Department of Chemistry, Utkal

University, Orissa, India

2001-2008 Research Associate, Experimental Endocrinology and Clinical Immunology Laboratory,

Wadsworth Center, New York State Department of health, ESP, Albany, NY

2009-present Research Affiliate, Department of Infectious Disease & Host Defense, Wadsworth Center,

New York State Department of Health, ESP, Albany, NY

HONORS

2007 Clinical Laboratory Technologist, State University of New York, Albany, New York

Clinical Laboratory Technologist Practicing license, State of New York, Albany, New York

2009 Membership, The New York Academy of Sciences, New York, New York, USA

SPECIAL COMPETENCIES AND INTERESTS

Cell biology / Immunology

• Excellent skills in Cell culture (Cell lines, whole blood, Mixed-Lymphocytes Culture, PBMC etc.) and Primary T cell isolation. Proliferation of T-cells by means [3H]-TdR incorporation. Bio-synthesis of cytokine by Radio-labeling Method. Detection of Cellular Thiol by Avidin Biotin Technology.

• Immunophenotyping by means of Flow cytometry. immunoassays, protein function assays, ELISA,

immunoprecipitation, Western / immunoblot, ELISPOT, and signal-transduction biology.

• Expertise in raising polyclonal and monoclonal antibodies (‘Hybridoma’ technology) against different antigens as well as maintaining a hybridoma core. Strong hands-on experience in using ‘Luminex’ for detection of cytokines from small volume of sample (Serum, cell culture supernatant, body fluids and tissue homogenate etc.) Experience with mouse models of autoimmune diseases. Extensive experience in using knockout mice

Proteomics

• Protein Purification and Characterization

• Purification of proteins by Column, Affinity-Chromatography

• 2D gel analysis.

• In-gel digestion for MS sample preparation.

• MALDI-TOF peptide fingerprint / Protein ID by Protein Prospector.

• Nano-ESI-LC-MS/MS (LTQ) for protein ID / PTM analysis by SEQUEST, Mascot.

• Labeling methods: SILAC, iTraq, 16O/18O.

• Sample enrichment methods: IMAC for phosphorylation, SCE.

Neuroscience

• Euthanasia of rodents, perfusion and whole brain extraction, followed by dissection into multiple coronal levels for fixation and embedding into paraffin blocks.

• Paraffin embedded tissue sectioning with microtomy and vibrotome for fresh tissue to generate sections ranging 5-100µm.

• Histological, immunocytochemical and immunofluorescent staining with multiple antigens of brain tissue, to look at region specific vascular, neuronal and/or glial cell degeneration and activation with respect to a variety of toxins, age and strain type.

• Brightfield and fluorescent microscopy and digital image acquisition.

• Use of image pro plus analytical software to quantify region specific pathological changes in brain tissue photomicrographs.

• Detection of neurotransmitters in fresh and frozen brain tissue; biogenic amines and their metaboliges: Dopamine, DOPAC, Homovanillic Acid Norepinephrine etc. using HPLC.

Molecule biology

Isolation of DNA and RNA, DNA and RNA electrophoresis, PCR, RT-PCR, situ hybridization, transfection, Southern and Northern analysis.

Microscopy

Light microscopy, fluorescent/laser confocal microscopy

Tumor biology

Animal models of tumor metastasis (intracardiac Injections, tail vein injection, mammary fat pad injection), colony formation, tumorigenecity, animal X-ray radiography, animal dissection

Pathology

• Detection of anemia, thalassemia, hemolysis, fetal hemoglobin, hemoglobin electrophoresis

(Starch-agarose), G6PD assay and other hemoglobinopathies. Malaria, filarial detection.

• Histological, serological and pathological diagnosis, techniques for sample/section preparation and

HE staining, immunohistological double/triple staining and Blood drawing.

Computer skills

Imaging and scanning by using Photoshop and NIH image software. Microsoft Word, Microsoft Excel, Power Point, Sigma plot and BLAST, comfortable on Windows

SELECTED PEER- REVIEWED PUBLICATIONS

1. Mondal TK, Gao D, Kasten-Jolly J and Lawrence DA (2011) Acute cold/restraint stress-induced inhibition of host resistance to Listeria monocytogenes infection: ER Stress response? (in preparation)

2. Mondal TK, Guo TL,Shenker BJ and Lawrence DA. (2011) Differential effects of mercury and lead on HLA-DR and invariant chain expression by human peripheral blood mononuclear cells. (in preparation)

3. Mondal TK, Gao D and Lawrence DA (2011) Differential protein synthesis and functional alterations of in vitro macrophage development by lead. (in preparation)

4. Miller VM, Lawrence DA, Coccaro GA, Mondal TK, Andrews K, Dreiem A, Seegal RF. (2010) Sex effects of interleukin-6 deficiency on neuroinflammation in aged C57Bl/6 mice Brain Res. 1318, 11-22.

5. Miller VM, Lawrence DA, Mondal TK, Seegal RF. (2009) Reduced glutathione is highly expressed in white matter and neurons in the unperturbed mouse brain--implications for oxidative stress associated with neurodegeneration. Brain Res. 1276, 22-30.

6. Mondal TK, Saha S, Miller VM, Seegal RF, Lawrence DA (2008) Autoantibody-mediated Neuroinflammation: Pathogenesis of Neuropsychiatric Systemic Lupus Erythematosus in the NZM88 Murine Model. Brain Behav. Immun. 22, 949-59.

7. Gao D, Mondal TK, Lawrence DA (2007) Lead effects on development and function of bone marrow-derived dendritic cells promotes Th2 immune responses. Toxicology and Applied Pharmacology. 222,69-79.

8. Heo Y, Mondal TK, Gao D, Kasten-Jolly J, Kishikawa H, Lawrence DA (2007) Post-Transcriptional Inhibition of Interferon-Gamma Production by Lead. Toxicol Sciences.96, 92-100.

9. Lawrence DA, Boliver VJ, Hudson C, Mondal TK and Pabello N (2007) Antibody induction of lupus-like neuropsychiatric manifestations. Journal of Neuroimmunology, 182,185-194.

10. Holub M, Lawrence DA, Mondal TK (2006) Effects of murine endotoxemia on lymphocyte subsets and clearance of staphylococcal pulmonary infection. Folia Microbiol (Praha), 51, 469-72.

11. Huber V, Mondal T, Factor SA, Seegal RF, Lawrence DA (2006) Antibodies in the sera of Parkinson's React with Neuronal Membrane Proteins and Affect Dopamine Expression by Neuronal Cells. Journal of Neuroinflamation, 3, 1-12.

12. Mondal TK, Li D, Swami K, Dean, Hauer C, Lawrence DA (2005) Mercury impairment of mouse thymocyte survival in vitro: involvement of cellular thiol. Journal of Toxicology and Environmental Health A, 68, 535-556.

13. Hudson CA, Mondal TK, Cao L, Kasten-Jolly J, Huber V, Lawrence DA (2005) The dietary supplement ephedrine induces b-adrenergic mediated exacerbation of systemic lupus erythematosus in NZM391 mice. Lupus, 14, 293-307.

14. Mondal TK, Bhatta D, Biswas S, Pal P (2002) Superantigen-induced apoptotic death of tumor cells is mediated by cytotoxic lymphocytes, cytokines, and nitric oxide. Biochem Biophys Res Commun, 290, 1336-42.

15. Mondal TK, Bhatta D, Biswas S, Pal P (2002) Repeated treatment with S. aureus superantigens expands the survival rate of Ehrlich ascites tumor bearing mice. Immunol Invest, 31,13-28.

16. Mondal TK, Bhatta D, Ray P, Pal P. (2001) Synergistic immunopotentiating effects induced by T-cell and B-cell superantigen in mice. Immunol Invest, 30, 169-80.

SELECTED LIST OF ABSTRACT AND CONFERENCE PSESENTATIONS

• Lawrence DA, Gao D and Mondal TK (2009) “Influence of cold-restraint stress on host defenses against Listeria infection’’, The 4th Cell Stress Society International (CSSI) Congress on Stress Responses in Biology and Medicine. Sapporo, Japan.

• Gao D, Mondal TK, and Lawrence DA (2006) In Vitro effects of lead on the development of bone marrow-derived dendritic cells”, The American Association of Immunologists, Annual Meeting, Boston, USA.

• Mondal TK, and Lawrence DA (2004) “Endotoxemia protects BALB/c from secondary staphylococcal infection of lungs”, Abstract Book, 17th ESCIME Annual Congress, Berlin, Germany.

• Holub M, Mondal TK, and Lawrence DA (2002) “Dis-regulation of Lung Immune responses of BALB/c Mice after Systemic Administration of Endotoxin”, Abstract Book, FOCIS Meeting, San Francisco, USA.

• Mondal TK, Li D, and Lawrence DA (2002) “Effects of Mercury and Other Heavy Metals on Apoptosis of Mouse Lymphocytes Through a thiol-dependent pathway”, Abstract Book, NESOT Annual Meeting, Pfizer, Groton, Connecticut, USA.

• Mondal TK, Mendoza A, Gao G, Andersen N, Ault J and Lawrence DA (2008) “Biomarkers signatures of biological, chemical and psychological stress”, Abstract Book, Departmental of Biomedical Sciences, Tenth Annual Research Conference, The Rensselaerville Institute, October 14th, Albany, New York, USA.

REFERENCES

• References available upon request.

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