Curriculum Vitae

Bushra E-mail :- cwvvho@r.postjobfree.com

M.Sc. in Biotechnology cwvvho@r.postjobfree.com

Hamdard University Female:- Single

New Delhi- 110 042 DOB:- 17th July 1985

India Contact No. +91-954*******

+91-882*******

OBJECTIVE

Looking for a challenging position in an organization, where I can enhance my skill and strengths in conjunction with the company’s goals and objective.

Professional Qualification

6 months Job Training (Hands-on) in i Life Discoveries in 2011.

M. Sc. (Biotechnology) from Hamdard University in 2009.

Cumulative Grade Point Average: 6.42.

Lab Experience and Skills

Hands-on training at i Life Discoveries, IMT Manesar, Gurgaon, in various techniques

Microarray experiments.

Phylogenetic Analysis and species identification by 16S rRNA method.

Isolation of DNA from plant, bacteria and animal tissue.

Polymer Chain Reaction (PCR), Q-PCR, RT-PCR.

Randomly amplified polymorphic DNA (RAPD).

Isolation of RNA from plant, animal tissue and blood sample.

Isolation of Plasmid DNA.

Mini prep, Midi prep and Gel purification.

For quality check Nano drop, Bioanalyzer, agarose gel electrophoresis, SDS PAGE profiling.

Competent cell preparation and storage.

Restriction mapping, restriction digestion and ligation.

Cloning, expression and transformation experiments.

Academic Qualification

1. Hands-on Job training (of 6 month) at i Life Discoveries, IMT Manesar, Gurgaon in 2011.

2. Computer course (Infoedge) from NIIT, New Delhi in 2010.

3. Master of Science (M. Sc.) in Biotechnology from the Department of Biotechnology, Jamia Hamdard University, New Delhi in 2009 with 6.42 Average Grand point.

4. Bachelor of Science (B. Sc.) with Industrial Microbiology (Major), Botany, Chemistry from KRG College, Jiwaji University, Gwalior in 2007 with 65.4% marks.

5. Diploma in Computer Application (DCA) in 2006.

6. Senior Secondary School Certificate (SSSC)/Intermediate of Science (I. Sc.) from M. P. Board in 2003 with 68.9% marks.

7. Secondary School Certificate (SSC)/Matriculation from M. P. board in 2001 with 65.4% marks.

Experimental Expertise

1. Molecular Biology:- Isolation of genomic DNA from bacteria (E. coli and Salmonella spp.) and plant tissues. Isolation of RNA, cDNA synthesis. PCR, Q-PCR. NanoDrop for Quality Check. Agarose and polyacrylamide (SDS and Native) gel electrophoresis, various blotting techniques such as Southern, Northern and Western. Extraction of Proteins from bacterial spp. and their SDS-PAGE profile. Competent cell preparation, Cloning, Transformation and expression. Mini Prep and Midi prep for plasmid isolation.

2. Bioinformatics:- Basic Windows based applications, Various Genomics and Proteomics tools, Sequence analysis, Phylogenetic analysis, familiar with different biological databases, Statistical/Graphing software like SigmaPlot and Origin, blast, fasta, primer designing, oligoanlyzer, gene annotation, gene scan and gene finding.

3. Animal cell culture:- Primary cell culture, Growth and maintenance of mammalian and insect cell lines, their transfection and selection.

4. Microbiology:- Growth and maintenance of bacterial cultures, transformation of bacteria by chemical and electroporation methods and selection, antibiotic sensitivity tests.

5. Immunology:- Study of inducible Nitric oxide synthase (iNOS) enzyme activity in peritoneal macrophages in mice, Raising the polyclonal antibodies in mice, Immunological techniques like ELISA.

6. Biochemistry:- Various chromatographic techniques like Gel filtration, Paper, TLC, Enzyme isolation from blood serum and its kinetic studies, Isoenzyme analyses, Qualitative and quantitative analyses of various macromolecules in biological samples, colorimetry & spectrophotometry.

7. Plant Biotechnology:- Regeneration of plant tissues on synthetic media, Protoplast isolation and fusion, transformation of plant cells and protoplasts and selection of transformants, Production of synthetic seed. Protein, Proline estimation, Chlorophyll estimation, NR activity and RAPD analysis of regenerants.

8. Plant physiology:- Nitrite, Nitrate and Nitrate reductase (NR) activity measurements in plants.

9. Computer knowledge:- MS office, MS word, MS Excel, advanced Excel, web designing etc.

Institutional Training

1. Hands-on job training (project) at i Life Discoveries, IMT Manesar, Gurgaon, Project worked on ‘Gene cloning and expression of ABHD11 gene HEK293 (human embryo kidney)’ and ‘Identification of bacterial species by 16s rRNA gene sequencing’ during the period of January 2011 to June 2011.

2. Dissertation (Project Major) at Department of Biotechnology, Hamdard University on ‘Effect of ZnSO4 on Taraxacum officinale and assessment of clonal fidelity through RAPD’ during the period of January 2009 to May 2009.

3. Summer training at Sanchi Dugdh Sangh, Banmore (M. P.) on ‘Microbial analysis of milk and milk products’ during May 2006 to June 2006.

Seminar Delivered

1. Presented my project work at the i Life Discoveries, IMT Manesar, Gurgaon.

2. Presented my dissertation work ‘Effect of ZnSO4 on Taraxacum Officinale and assessment of clonal fidelity through RAPD' at the Department of Biotechnology, Hamdard University, New Delhi in May 2009.

3. Delivered a seminar on 'Hypersensitivity and related diseases' during my Masters in Biotechnology at the Department of Biotechnology, Hamdard University, New Delhi in August 2008.

4. Delivered a seminar on 'Food Microbiology' during my Masters in Biotechnology at the Department of Biotechnology, Hamdard University, New Delhi in February 2008.

5. Presented my summer training work 'Microbial analysis of milk and milk products' at Department of Microbiology, KRG College, Gwalior in January 2003.

6. Delivered a seminar on 'Micro-organisms in our daily life' at Department of Microbiology, KRG College, Gwalior in January 2002.

Extra Curricular Activities

• Participated in the Nanotechnology seminar delivered by Dr. Eric M. Drexler (called Father of Nanotechnology) and seminar on Intellectual Property Rights organized by NRDC.

• Have experience of arranging various cultural programmes at school and college level.

References

• Prof. M. Z. Abdin

Head, Transgenic Plants laboratory

Department of Biotechnology, Faculty of Science

Hamdard University, New Delhi - 110 062

Email: cwvvho@r.postjobfree.com

• Vipul Bhargava, Ph. D

Associate Director, i Life Discoveries

154S, Sector 7, IMT Manesar,

Gurgaon – 122 050

Email: cwvvho@r.postjobfree.com

Personal Information

Father’s name : Mr. S. M. Afaq

Date of Birth : 17th July 1985

Nationality : Indian

Languages known : Hindi, English, Urdu

Permanent Address : N-45, Sailing Lane 2, Near Masjid Khalilullah, Batla House, Jamia Nagar,

Okhla, New Delhi – 110 025

Contact Nos. : 011-********/+91-954*******/+91-882*******

Date : (Bushra)

Place: New Delhi

Contact this candidate