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Maintenance Technician

Location:
Candia, NH
Salary:
60,000/yr
Posted:
November 27, 2012

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Resume:

Jennifer Hewitson, HTL (ASCP)CMP

***********@*****.***

** ******** *******

Hampton, NH 03842

781-***-****

EDUCATION

-Certificate in Histotechnology (March, 2006)

Barry University, Miami Shores, FL GPA: 3.98

-Master of Science, Molecular and Cellular Biology (September, 2000)

University of Massachusetts, Amherst, MA GPA: 3.70

-Bachelor of Science, Biochemistry and Molecular Biology (May, 1998)

University of Massachusetts, Amherst, MA GPA: 3.56

RELAVENT EXPERIENCE

Catholic Medical Center, Manchester, NH

Histotechnologist

May 2010-present

Techniques/Responsibilities: Processing, embedding, sectioning and routine

staining of tissue. Grossing biopsies, immunohistochemistry (Leica Bond Max

and III), developing new IHC protocols

Caris-Cohen Diagnostics, Newton, MA

Histotechnologist/Grossing Technician

Aug 2008-April 2010

Techniques/Responsibilities: Processing, embedding, sectioning and routine

staining of human tissue (GI and derm specimens mainly), Grossing GI and

derm biopsies, prescreening and imaging GI and derm slides for pathologists

DNAR (now On-Q-ity), Waltham, MA

Research Scientist, Part time

May 2008-July 2009

Techniques/Responsibilities: Sectioning paraffin-embedded tissue; database

entry and image analysis/ prescreening/identifying areas of interest in

microarray tissue images for pathologist (Aperio & ImageScope),

immunohistochemistry assays (automated-LabVision)

The Lahey Clinic, Burlington, MA

Histotechnologist, Histology Lab

May 2006-July 2008

Techniques: paraffin embedding and sectioning of tissue specimens; H&E

staining and coverslipping (automated); special stains (automated-Dako);

immunohistochemistry, daily(automated-Dako); Fluorescent In Situ

Hybridization (FISH), Her2-Neu routinely, as well as new protocol

development (all by hand)

Responsibilities: maintaining laboratory notebook for experimental FISH

results, documentation of Her2-FISH reports, making buffers and solutions

for FISH, maintenance of supplies for the Immunohistochemistry and FISH

labs, general lab utility duties, including maintenance of microwave tissue

processors(Milestone Pathos), and loading and unloading specimens on

processors

Holy Cross Hospital, Fort Lauderdale, FL

Clinical Internship, Histology Lab

Aug 2005-March 2006

Techniques: embedding and sectioning paraffin-embedded tissue, including

biopsies and routines, as well as bone; cryosectioning; special stains (by

hand); immunohistochemistry (automated-Dako); H&E staining and

coverslipping (both by hand and with automation); some experience grossing;

assisted two autopsies

Oregon Health and Science University, Portland, OR (PI: Malcom Low, MD,

PhD)

Research Associate, The Vollum Institute

Nov 2002-May 2004

Techniques: dissection of the mouse brain, hypothalamus and pituitary,

cryosectioning of mouse tissue, in situ hybridization, perfusion in mice,

sectioning of fixed tissue with vibratome, immunohistochemistry,

characterization/verification of gene expression in transgenic mouse lines

using PCR and immunohistochemistry, and Southern blotting,

primary cell culture of neurons, light and fluorescence microscopy

Responsibilites: breeding and genotyping of transgenic mouse colonies,

compliance and record-keeping for chemical and radiation safety, ordering

instruments and supplies, making buffers and solutions, documentation of

experiments and results in laboratory notebook

Phylos, Inc., Lexington, MA

Research Associate II - Proteomics

April 2001-Oct 2002

Massachusetts General Hospital, Boston, MA (PI: Laurence Rahme, PhD)

Research Associate, Dept. of Molecular Biology

Aug 2000-April 2001

University of Massachusetts, Amherst (PI: Dr. Randall W. Phillis, PhD)

Undergraduate and Graduate studies, Dept. of Biology

May 1997-Aug 2000



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