Assistant Data

HEVAN PATEL

**** ******* ***** • Morrisville NC *27560

cd0qcy@r.postjobfree.com • 919-***-****

RESEARCH ASSOCIATE: BIOTECHNOLOGY & MOLECULAR BIOLOGY

Proven capacity for employing analytical chemistry, molecular biology and protein purification techniques to conduct research into leading-edge biochemistry and biotechnology scientific advances. Highly adept at generating high-quality samples and compiling sophisticated data on results of analyses. Consistently remain ahead of schedule in fast-paced independent and team-based environments. Excel at operating, calibrating and maintaining inventories of sophisticated laboratory equipment. Poised, articulate and knowledgeable in delivering presentations and contributing to authoring of scientific articles. Multilingual in English, Hindi, Gujarati, and Sanskrit.

Core Capabilities & Interests

• Data Compilation / Analysis

• Good Manufacturing Practices (GMP)

• Quantitative / Qualitative Analysis

• Oncology Clinical Research • Molecular Biology Techniques / Processes

• Good Laboratory Practices (GLP)

• Molecular Genetics & Diagnostics

• Regulatory Compliance

EDUCATION AND CREDENTIALS

Master of Science, Biotechnology (2011)

NORTHEASTERN UNIVERSITY – Boston, Massachusetts

Bachelor of Science, Pharmacy (2008)

NAVODAYA PHARMACY COLLEGE – Bangalore, India

CAREER EXPERIENCE

NORTHEASTERN UNIVERSITY ANTIMICROBIAL DISCOVERY CENTRE, Boston, Massachusetts

Research Associate, 1/2011 – 12/2011

Contributed extensively to all phases of high-complexity molecular laboratory tests. Generated / compiled test results and tracked testing records. Conducted statistic evaluations of data. Reported regularly to team members and presented data at laboratory meetings. Secured and maintained inventory of testing samples. Utilized various equipment, including thermocycler / PCR machine, Qiagen kits, Invitrogen ligation kits, and autoclave machine.

Key Contributions:

• Planned and executed SOE-PCR, produced toxin-antitoxin module, and performed P. aeruginosa DNA knock out.

• Inserted knocked out toxin-antitoxin DNA in pUC 18 plasmid via Gateway cloning.

• Characterized correct insertion through gene sequencing and expression in E. coli.

• Produced toxin-antitoxin and deleted P. aeruginosa by cloning and analyzing its susceptibility to causing cystic fibrosis.

• Demonstrated capacity for performing and conducting in-depth analysis on results of highly complex molecular biology techniques while under minimum supervision.

NORTHEASTERN UNIVERSITY PHARMACEUTICAL SCIENCES LAB, Boston, Massachusetts

Graduate Research Assistant, 1/2011 – 4/2011

Rapidly gained exposure to, applied and troubleshot new molecular biology techniques. Collaborated closely with world-class team of five. Formulated weekly lab reports. Operated NanoDrop for nucleic acid quantification, Biotek synergy HT plate reader and fluorescence microscope.

Key Contributions:

• Played key role in project entailing design of new pharmaceutical for treating acute renal failure.

• Inserted transcription factor into pIRES-zsGreen1 and analyzed via gel electrophoresis.

• Cultured and maintained COS-7 cell line for cloning and expression.

• Cloned COS-7 cells and expressed desired transcription factor for analyzing use in acute renal failure.

• Consistently remained ahead of schedule within highly deadline-driven environment.

BIOTECHNOLOGY APPLICATION LAB, Boston, Massachusetts

Graduate Research Assistant, 1/2010 – 5/2010

Conducted independent research and collaborated with peers in laboratory research and presentation. Partnered with supervisor in preparing researching findings for publication. Acquired expertise in key scientific tools and techniques such as cloning, protein expression / purification and western blotting apparatus. Utilized affinity, gel filtration and ion exchange methods to conduct protein purification.

Key Contributions:

• Separated and identified proteins through use of SDS PAGE, native PAGE, ELISA, western blot and porous chromatography; additionally determined concentration of proteins via BCA assay and Bradford’s assay.

• Successfully performed protein quantification by employing HPLC and RPLC and analyzing varying classes, subclasses, and individual lipid species from biological extracts using ESI-MS.

INDICA LABORATORIES LTD., Ahmedabad, India

Laboratory Technician, 10/2008 – 6/2009

Performed a wide variety of in vitro and in vivo tests, and maintained records to ensure compliance with regulatory guidelines. Maintained and stocked laboratory inventory. Liaised with vendors to procure laboratory equipment and materials.

Key Contributions:

• Skillfully injected various formulations in mice for acute toxicity testing, extracted DNA/RNA and amplified via PCR for further analysis.

• Regularly remained well ahead of deadline while demonstrating exemplary analytical and problem-solving skills and meeting compliance objectives.

TECHNICAL PROFICIENCIES

Platforms: Windows XP/Vista/7

Software: LGC Genomics Primer Design, Gen5 Microplate Reader, SPSS, MS Office (Word, Excel, Access, Outlook, PowerPoint)

Tools: Nano Drop1000,HPLC,Fluorescence Microscope, Gel Imager Carestream 112

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