YUMIN CHEN, PH.D.

513-***-**** • **** S. White Oak Drive Apt. 625, Waukegan, IL 60085 • c7x4d8@r.postjobfree.com

Dedicated professional with a solid background in Antioxidant Research, interested in the areas of food chemistry, free radical chemistry, biology, pharmacology, and toxicology. Adept at scientific writing and presenting research data; poised to promptly comprehend and employ new concepts and skills. Committed team-player comfortable working in a diverse environment. Key career achievements include nine presentations at international conferences, seven first-authored papers, six co-authored papers, two papers submitted for review, and one patent. Depth of professional experience includes:

 Various research techniques, including Antioxidant Characterization, Flow cytometry, HPLC, ESI MS, MALDI-TOF MS, proteomics, EPR, UV/Visible, Fluorescent, and IR spectroscopy

 Animal sample preparation and isolation of organelles (e.g. mitochondria)

 Cloning, PCR, protein purification, and other routine molecular biology techniques

 Proficient in PDQuest and GraphPad Prism as well as Microsoft Word, Excel, PowerPoint - able to learn proprietary systems / applications quickly and easily

 Serves as ad hoc reviewer of Journal of Agriculture and Food Chemistry

EDUCATION

MIAMI UNIVERSITY, Oxford, Ohio

Doctor of Biochemistry, 2004

Dissertation: Oxidation of Polymeric Polyphenols (tannins) in Biologically Relevant Systems

CHINESE ACADEMY OF SCIENCES - INSTITUTE OF OCEANOLOGY, Qingdao, China

Master of Science in Marine Chemistry, 1999

Thesis: Hydroxyl Characterization and Antioxidant Activity of Seaweed Polyphenols

SHANDONG LIGHT INDUSTRY COLLEGE, Jinan, China

Bachelor of Science in Food Engineering, 1996

Thesis: Enzymatic Preparation of Gelatin from Bovine Bone

PROFESSIONAL EXPERIENCE

VIRGINIA POLYTECHNIC INSTITUTE (VIRGINIA TECH), Blacksburg, Virginia 2007 – Present

Postdoctoral Researcher

▪ Study cytotoxicity of polyamine-based gene delivery materials in H9c2 (2-1) cells. Discovered polyamines induces the death of H9c2 (2-1) cells via a cathepsin D mediated mechanism and specific inhibition of cathepsin D promotes H9c2 (2-1) cells survival by 50%.

UNIVERSITY OF KENTUCKY, Lexington, Kentucky 2005 – 2007

Postdoctoral Researcher

▪ Discovered pathological biomarkers for anticancer drug, doxorubicin, through Redox proteomics methodology. Treated mice with doxorubicin, isolate heart or heart mitochondria for redox proteomics and enzymatic characterization of the specifically oxidized metabolic proteins.

• Conducted 2D electrophoresis of cardiac proteins, image analysis (PDQuest) of 2D gels and 2D Western blots, and identification of proteins via peptide MS fingerprinting. Characterized proteins oxidation biomarkers, including carbonyl, HNE and nitrotyrosine, and observed signaling pathways using mammalian cell culture.

▪ Studied modulation of cardiotoxicity of Doxorubicin with histone deacetylase inhibitors. Discovered in a mouse model phenylbutyrate, a histone deacetylase inhibitor can protect heart against Doxorubicin cardiotoxicity.

▪ Studied cardiac mitochondrial targeting small molecules (superoxide dismutase mimetic). Discovered Mn TE-2-PyP5+, a potent antioxidant, targets cardiac mitochondria. Observed pharmacokinetics of Mn TE-2-PyP5+ in mouse plasma, liver, kidney, spleen, heart, lung, and brain.

CHILDREN’S HOSPITAL MEDICAL CENTER, Cincinnati, Ohio 2004

Postdoctoral Researcher

▪ Studied transcription activator-DNA interaction. Applied molecular biology and protein chemistry to study a family of transcription activators (Six protein, Six 1-6) and determined protein structures responsible for DNA binding.

▪ Cloned site-directed mutagenesis to express proteins in bacteria and purify proteins for protein-DNA interaction study and crystallization. Applied EMSA assay to study protein-DNA binding.

MIAMI UNIVERSITY, Oxford, Ohio 1999 – 2004

Doctoral Researcher

▪ Studied oxidation pathways of nutritional antioxidant (polyphenols) in biological matrix. Isolated and purified polyphenols, and characterized protein-polyphenol interaction, oxidation products, and polyphenol pathways.

▪ Conducted ESI MS and MALDI-TOF MS analysis of polyphenols and polyphenol-protein complexes.

▪ Developed HPLC method for polyphenol analysis and synthesized 14C-labeled polyphenols.

▪ Created quantitative analytical method to separate / analyze polyphenol oxidation products and polyphenol-protein complexes. Discovered two pathways in polyphenol oxidation under the control of pH and protein.

Teaching Assistant

▪ Prepared and presented laboratory lectures in general chemical laboratories; participated in course development and student mentoring and evaluated student performance through exams / lab efficiency. Coordinated undergraduate students on special projects.

CHINESE ACADEMY OF SCIENCES - INSTITUTE OF OCEANOLOGY, Qingdao, China 1996 – 1999

Graduate Researcher

▪ Studied antioxidative seaweed polyphenols and their biosynthesis; isolated and purified polyphenols from seaweed; characterized polyphenol antioxidant activity and studied structure-antioxidant activity relationship.

▪ Conducted biosynthesis of seaweed polyphenols (Oceanol. Liminol. Sinica 1997. 28, 225-232) and one patent (CN1202334, Dec. 23, 1998) on application of seaweed polyphenols as food antioxidant.

▪ Developed analytical method to study the structural-antioxidant activity relationship for polyphenols.

AWARDS / AFFILIATIONS

MIAMI UNIVERSITY, Oxford, Ohio

William Hale Charch Graduate Fellowship, 2000, 2001, & 2002

AMERICAN CHEMICAL SOCIETY

SOCIETY OF FREE RADICAL BIOLOGY AND MEDICINE

Member

PRESENTATIONS

Spasojevic, I., Chen, Y., Noel, T.J., Fan P., Zhang, L., Reboucas, J.S., St. Clair, D.K., and Batinic-Haberle, I. Potent Redox Modulator of Oxidative Stress, MnTE-2-PyP5+. Pharmacokinetics in Plasma, Liver, Kidney, Lung, Heart, Spleen, and Brain. 14th Annual Meeting of the Society for Free Radical Biology and Medicine, Washington, D.C., November 14-18, 2007. (Poster)

Sompol, P., Ittarat, W., Tangpong, J., Chen, Y., Doubinskaia, I., Batinic-Haberle, I., Abdul, H.M., Butterfield, D.A., and St. Clair, D.K. Overproduction of beta-amyloid peptides induces chronic oxidative stress and suppresses MnSOD expression in mature neurons: implications for Alzheimer's disease. 14th Annual Meeting of the Society for Free Radical Biology and Medicine, Washington, D.C., November 14-18, 2007. (Poster)

Spasojevic, I., Batinic-Haberle, I., Yu, Y., Chen, Y., Noel, T.J., Cole, M.P., Zhao, Y., and St. Clair, D.K. Mn porphyrin-based SOD mimic, MnTe-2-PyP5+ (AEOL-10113), targets mouse heart mitochondria after single 10 Mg/Kg IP administration. 13th Annual Meeting of the Society for Free Radical Biology and Medicine, Denver, CO, November 15-19, 2006. (Poster)

Chen, Y., Daosukho, C., Opii, W., Klein, J.B., Butterfield, D.A., Vore, M., and St. Clair, D.K. Cardiac mitochondrion is an early target of taxol. 12th Annual Meeting of the Society for Free Radical Biology and Medicine, Austin TX, November 16-20, 2005. (Poster)

Daosukho, C., Noel, T., Lien, Y.C., Chen, Y., and St. Clair, D.K. Manganese superoxide dismutase (MnSOD) deficiency enhances adriamycin (ADR) and paclitaxel (Taxol)-induced p53 levels in heart mitochondria. 12th Annual Meeting of the Society for Free Radical Biology and Medicine, Austin TX, November 16-20, 2005. (Poster)

Chen, Y. and Hagerman, A.E. Tannin-protein interactions in the gastrointestinal milieu. 228th ACS National Meeting, Philadelphia, PA, August 22-26, 2004. (Talk)

Hagerman, A.E. and Chen, Y. Oxidation of polymeric polyphenols in biological matrix. First International Congress on Antioxidant Methods, Orlando, FL, June 16-18, 2004. (Poster)

Chen, Y. and Hagerman, A.E. Oxidation of polymeric polyphenols in biological matrix. 227th ACS National Meeting, Anaheim, CA, March 28-April 1, 2004. (Poster)

Hagerman, A. E., Niemeyer, L., Forkner, R., Chen, Y., Minto, R. E., and Ansong, G. New chemical methods for studying polymeric polyphenols (tannins). 19th Annual Meeting of the International Society of Chemical Ecology, University of Hamburg, Germany, August 3-8, 2002. (Poster)

PUBLICATIONS

Jungsuwadee, P., Nithipongvanitch, R., Chen, Y., Oberley, T.D., Butterfield, A., St. Clair, D.K., and Vore, M. Mrp1 Localization and Function in Cardiac Mitochondria Following Doxorubicin. (Submitted to Molecular Pharmacology)

Sompol, P., Ittarat, W., Tangpong, J., Chen, Y., Doubinskaia, I., Batinic-Haberle, I., Abdul, H. M., Butterfield, D. A., and St. Clair, D.K. Overproduction of beta-amyloid peptides induces chronic oxidative stress and suppresses MnSOD expression in mature neurons: Implications for Alzheimer’s disease. Journal of Neuroscience Research, 2007. (Submitted)

Dey, S., Bakthavatchalu V., Pseng, M.T., Wu, P., Stefanski, R., Grulke, E.A., Yokel, R., Dhar, S., Yang, H.S., Chen, Y., and St. Clair, D. K. Interactions between SIRT1 and AP-1 reveal a Mechanistic Insight into the Growth Promoting Properties of Alumina (Al2O3) Nanoparticles in Mouse Skin Epithelial Cells. Carcinogenesis, 2008. (Published online)

Spasojevic, I., Chen, Y., Noel, T.J., Fan, P., Zhang, L., Reboucas, J.S., St. Clair D.K., and Batinic-Haberle, I. Pharmacokinetics of the potent redox-modulating manganese porphyrin, MnTE-2-PyP(5+), in plasma and major organs of B6C3F1 mice. Free Radical Biology & Medicine, 45 (7): 943-49, 2008.

Liu, B., Chen, Y., and St. Clair, D.K. ROS and p53: versatile partnership. Free Radical Biology & Medicine, 44 (8): 1529-35, 2008.

Chen, Y., Jungsuwadee, P., Vore, M., Butterfield, D.A., and St. Clair, D.K. Oxidative stress-induced normal tissue injuries: a complication of anticancer drugs. Molecular Interventions, 7(3):147-156, 2007.

Daosukho, C., Chen, Y., Noel, T., Sompol, P., Nithipongvanitch, R., Velez, J.M., Oberley, T.D., and St. Clair, D.K. Phenyl butyrate, a histone deacetylase inhibitor, protects against Adriamycin-induced cardiac injury. Free Radical Biology & Medicine, 42 (12), 1818-1825, 2007.

Spasojevic, I., Chen, Y., Noel, T.J., Yu, Y., Cole, M.P., Zhang, L., Zhao, Y., St. Clair D.K., and Batinic-Haberle, I. Mn porphyrin-based superoxide dismutase (SOD) mimic, Mn TE-2-PyP5+, targets mouse heart mitochondria. Free Radical Biology & Medicine, 42(8), 1193-1200, 2007.

Chen, Y., Daosukho, C., Opii, W.O., Turner, D.M., Pierce, W.M., Klein, J.B., Vore, M., Butterfield, D.A., and St. Clair, D.K. Redox proteomic identification of oxidized cardiac proteins in Adriamycin-treated mice. Free Radical Biology & Medicine, 41(9), 1470-1477, 2006.

Dhar, S.K., Xu, Y., Chen, Y., and St. Clair, D. K. Specificity Protein 1-dependent p53-mediated Suppression of Human Manganese Superoxide Dismutase Gene Expression. Journal of Biological Chemistry, 281(31), 216**-*****, 2006.

Chen, Y. and Hagerman, A.E. Reaction pH and Protein Affect the Oxidation Products of β-pentagalloyl Glucose. Free Radical Research, 39(2), 117-124, 2005.

Chen, Y. and Hagerman, A.E. Quantitative Examination of Oxidized Polyphenol-Protein Complexes. Journal of Agricultural and Food Chemistry, 52(20), 6061-6067, 2004.

Chen, Y. and Hagerman, A.E. Characterizing Soluble Non-covalent Complexes between Bovine Serum Albumin and β-1,2,3,4,6-penta-O-galloyl-D-glucopyranose by MALDI-TOF Mass Spectrometry. Journal of Agricultural and Food Chemistry, 52, 4008-4011, 2004.

Chen, Y. and Hagerman, A.E., Minto, R. E. Preparation of 1,2,3,4,6-penta-O-galloyl-[U-14C]-D-glucopyranose. Journal of Labeled Compounds and Radiopharmaceuticals, 46, 99-105, 2003.

Chen, Y., Yan, X., and Fan, X. Hypothesis on Phaeophyceae Polyphenols: Their Structural Unit and Mechanism of Formation. Oceanologia et Limnologia Sinica, 28, 225-232, 1997.

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