Assistant Software

RUCHI PATEL

*** ***** ***, ************, ** *****

: 732-***-****, Email: *********@*****.***

OBJECTIVE:

Team-oriented, creative and technically experienced Molecular Biologist seeking research and development position in the Biotech industry.

EDUCATION:

Bachelor of Science in Biology with Distinction, GPA 3.4 Graduated May -2009

Temple University, Philadelphia, PA

RESEARCH SKILLS:

RNA Isolation/Purification cDNA Generation

Cell and Tissue Culture

Vector Cloning

Bacterial Cell Transformation

PCR, RT-PCR and Q-PCR

Plasmid/ Genomic/ PCR Purification/ Quantitation

Fluorometric Assays (Picogreen/Ribogreen)

Southern Blotting/ Western Blotting

Emulsion PCR and Nested PCR

DNA extraction from human and mouse samples

Agarose Gel electrophoresis

Fluorescent In Situ hybridization (FISH)

Fluorescence microscopy/ Phase Contrast/ Bright field microscopy

INSTRUMENTATION:

ABI 3730 XL DNA Sequencer

Roche 454 Genome Analyzer

BioMek FX/NX Robotics

Promega Maxwell system

Beckman Coulter Counter

/ Nanodrop 8000/1000 Spectrophotometer

Spectramax M2

Thermocyclers

Qiagen Biocube

AB 7900 HT (real time PCR high throughput detector)

EMPLOYMENT:

06/2009 – 12/2010 and current: GlaxoSmithKline, Collegeville, PA

Associate Scientist –Temp-Platform Technology & Science, DNA Sequencing Group

• Provided sequence characterization for Dengue, MUMPs, and VZV viral passages for Belgium Biologicals.

Assembled and analyzed Sanger sequences using Sequencher® 4.8 software to identify mutations.

Generated data, participated in team meetings to discuss results with Belgium team.

• Biocat Cell line Authentication studies to support BRAD:

Performed the identification and authentication of human cell lines and detection of intra-species cell line cross contamination.

Analyzed the Loss of Heterozygosity, Microsatellite and SNP genotyping results using GeneMapper® 4.0 software.

Generated scientific reports for all cell lines

• Team member contributing to Next Generation Sequencing to support ID CEDD HCV DPU (HCV polymerase pan genotype inhibitor) to identify resistant mutants in HCV replicon samples after selection compounds.

• Team member contributing to Next generation sequencing (454 & illumina) to support Rotarix vaccine characterization for Belgium Biologicals.

• Team member for DNA sequencing support to specific projects from PHL based CEDDs/DPUs including Oncology, Metabolic II, Microbiome and GSK Biologicals and contributed to target & molecule progression.

Support daily Customer Core Sequencing efforts

• Records experimental data and maintain laboratory notebook.

• Expert in experimental design and data analysis.

• Actively participate and present in MCT meetings.

• Experience writing and proof reading SOP’s

• Have ability to learn new techniques, perform multiple tasks simultaneously, keep accurate records, follow instructions, and comply with company policies.

• Have ability to work independently and as a part of team, self motivation, adaptability and positive attitude.

08/2008 – 06/2009 Temple University Research Labs, Philadelphia, PA

Research Assistant

Zebra fish Lab

• Conducted bacterial genetics techniques, recombinant DNA techniques, including the ligation of gene clones, plasmid DNA extraction, DNA digestion with restriction enzymes, Agarose gel electrophoresis, Southern blot, Western blot, RT-PCR, the preparation of competent bacteria, ELISA and aseptic technique.

• Participated in the Zebra Fish research section of the laboratory acquiring the skill of construction of expression vectors, Microinjection in fish embryo and analyzed using fluorescent microscopy.

04/2008 – 05/2009 Montgomery Community College, Pottstown, PA

Microbiology Lab Assistant

• Responsible for preparing all media, cultures, microbial slides, solutions and set up labs for microbiology classes using Aseptic techniques.

• Assist faculty with development of demonstrations and experiments.

• Responsible for checking inventory.

• Assist students in troubleshooting lab problems.

COMPUTER SKILLS:

• Microsoft Office: Word/Excel/PowerPoint

• Sequence Analysis Software: Sequencher® 4.8, Laser gene, Codon Codes, Variant Reporter, CLUSTAL W, FASTA, BLAST, GeneMapper® 4.0, Vector NTI

• Nautilus and Sequencing LIMS Systems

REFERENCE:

• Available upon request.

Contact this candidate