Quality Control Engineer
Resume:
Anna Fomina
Parrish, FL *4219
***********@*****.***
WORK HISTORY
• Production Scientist, R&D Scientist, Quality Control Analyst, Life Sciences Advanced Technologies Inc., St. Petersburg, Florida: March 2011 to present
• Quality Control Analyst and R&D Scientist, Life Sciences Inc., St. Petersburg, Florida:
2008-2009
• Biochemist, College of Medicine, Department of Molecular Medicine, University of South Florida, Tampa; Principal Investigator – Gloria Ferreira, PhD, Tampa, Florida:
2007 - 2008
• Biochemistry Engineer, Laboratory of Biochemical Genetics at Institute of Biology, St. Petersburg State University, St Petersburg, Russia: 2003-2006
• Research Fellow, Department of Biochemistry, St. Petersburg Institute of Physical Culture and Sports, St. Petersburg, Russia: 2002-2003
EDUCATION
• Masters Degree in Biochemistry, Saint-Petersburg State University, Saint-Petersburg, Russia: 2002
• Bachelors Degree in Biology, Saint-Petersburg State University, Saint-Petersburg, Russia: 2000
PROFESSIONAL ACCOMPLISHMENTS
Production Scientist
Responsible for whole cycle of preparation of AMV (Avian Myeloblastosis Virus) Reverse Transcriptase from Avian Myeloblastosis Virus.
R&D Scientist
Successfully accomplished project of developing a new method of purification of T7 RNA Polymerase from E. coli.
Responsible for a project of developing a new method of purification of RNase H from E. coli.
Accomplished project of comparison of three plasmids containing three different mutations in gene of Reverse Transcriptase from Moloney murine leukemia virus (RT MMLV ) using a technique of transformation E. coli cells and further analysis of enzymatic activity of Reverse Transcriptase after partial purification.
Quality Control Analyst
Provided routine and non-routine analysis of raw materials and/or finished product (T7 RNA Polymerase, AMV Reverse transcriptase, RNase H, NASBA cocktail), as well as inspection testing of components to be used in further manufacturing for final product release.
Maintained databases for quality control and ensured all supporting tasks are completed within the lab.
Biochemist
Successfully accomplished three research projects for the Department of Molecular Medicine, University of South Florida, Tampa.
Projects consisted of creating and characterization new variants of enzyme ALAS (erythroid aminolevulinate synthase and 5-Aminolevulinate synthase), the first enzyme of the heme biosynthetic pathway in mammalian cells. Research includes producing new variants of ALAS using site-direct mutagenesis, finding methods of purification of new variants and characterization of catalytical properties of enzyme’s variants using steady-state kinetic assay.
Biochemistry Engineer
Expression of the interferon-beta gene in Pichia pastoris. Elucidated the role of aggregation caused by hydrophobic, ionic and Van der Waals interactions on stability, biological activity and immunogenicity of interferon. Elaborated the optimal conditions allowed the recombinant protein existence in the monomeric and dimmeric forms enhancing purification and providing high biological activity of it. Proposed a method of purifying high active form of interferon beta.
Research Fellow
Developed biomarkers for the monitoring of metabolic changes during exercise. Determined creatinkinase and aspartataminotransferase as a putative markers for muscles recovering. Discovered effects of media derived from muscle soleus after 1-5 days of exercise on adhesive characteristics and viability of myoblasts in cell culture. Provided investigation of myoblasts proliferation and translation activities elicited by conditioning media obtained during muscle recovering. Improved the organization and inventory control methods for the laboratory.
OBJECTIVE
Dedicated professional with strong credentials and extensive laboratory experience seeking career to maximize performance and training experience.
SUMMARY OF QUALIFICATIONS
Results-oriented, high-energy, hands-on professional, with a successful record of accomplishments in protein biochemistry. Experience in over expression of proteins in yeasts and E. coli; including utilization of radioactive labeling, enzyme kinetics, and cells culture work. Strong propensity on method development for isolation and purification of recombinant proteins inclined to pharmacy applications.
Major strengths include excellent communication skills, competent, strong team player, attention to detail, dutiful respect for compliance in all regulated environment. Possesses thorough knowledge of current research practices, and a clear vision to accomplish goals.
List of publications:
1. Sokolov I.N., Matushichev V.B., Fomina A.A. Adaptive moves in the protein pool of the Hen’s myoblasts culture induced by estradiol. THE 2th MEETING OF THE RUSSIAN ACADAMY OF SCINCE SOCIETY, Russia.- May 19-23, 1997.
2. Fomina A.A., Smirnov M.N., Padkina M.V. Getting of monomeric form of recombinant human interferon-beta from culture medium of yeast Pichia Pastoris. The third Moscow International Congress “Biotechnology: State of Art and perspects of Development”, Russia. March 14-18, 2005.
3. Fomina A.A, Smirnov M.N., Padkina M.V. Getting of monomeric high active form of recombinant human interferon-beta from culture medium of yeast Pichia Pastoris. The conference “ Genetics of microorganisms and Biotechnology”, Russia, Moscow, 2006.
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