Aditi Sharma

*** *. ******* ******; Apt. **** • Baltimore, MD 21201

225-***-**** • bhv1v5@r.postjobfree.com

Profile

BIOMEDICAL ENGINEER

MS in Biomedical Engineering with proven skills in assay and platform validation, collaborating with academic and industrial partners, and garnering published insights. Highly skilled in facilitating in vitro and in vivo studies, conducting cytotoxicity testing, and executing siRNA delivery studies. Expert communicator with a passion for implementing viable SOPs, effectively training research staff, and offering innovative solutions to project challenges.

Core Competencies: Research Project Planning/Implementation, Team Coordination, Analytical Problem Solving, Regulatory Compliance, Risk Management, Grant & Technical Writing Expertise, Presentations, Time Management

Laboratory Skills: Mammalian Cell Culture, PCR, HPLC, Microscopy, Histology, ELISA, Chromatography, Craniomaxillofacial Engineering, Protein Purification, Bioreactor Design, Western Blot, Northern Blot, Cellular Assay Techniques, Gel Electrophoresis, Plate readers for Fluorescence-Based Analysis

Relevant Experience

Career Note: Liaised heavily with clients in executing Phase I Environmental Assessments as Consultant for MS Environmental Consultants (1/11-Present, 8/07-8/08).

CARNEGIE MELLON UNIVERSITY

Pittsburgh, PA 8/08-12/10

Graduate Student Researcher & Lab Instructor

Analyzed and formulated growth factor and biomaterial combinations enabling maximum extent of bone regeneration for orthopedic research, developed targeted SOP documents for all assays and procedures, and garnered insights resulting in publications. Demonstrated versatility in cytotoxicity testing, biomaterial development, and grant writing for DoD-administered research grants. Collaborated effectively with lab members and offered key insights.

Key Accomplishments:

• Spurred development of nanoparticle system for siRNA delivery for treatment of heterotopic ossification through in-depth cytotoxicity testing of nanoparticles using hMSC and MC3T3 cells.

• Evaluated biocompatibility and osteogenic potential of scaffold formulations through Live/Dead stain and cell attachment evaluation via SEM image analysis, ADMET studies, mineralization and proliferation assays including calcium content, von Kossa stain, alkaline phosphatase (ALP) assay, total protein and picogreen assays.

• Developed Standard Operating Procedure (SOP) documents and trained lab members.

• Facilitated animal surgeries for in vivo studies investigating scaffold efficacy for bone regeneration in skull via implantation of biomaterial scaffolds.

• Enhanced cell culture media formulations and enriched cell cultures for selected cell types from tissue samples.

• Played key role in grant writing for DoD-administered research grants, presented at Armed Forces Institute of Regenerative Medicine (AFIRM) meetings, and produced detailed documentation on research findings.

LEADERSHIP HIGHLIGHTS:

• Managed annual research symposium for graduate BMES chapter as Vice President of Graduate Biomedical Engineering Society (8/08-10/09) for Carnegie Mellon University. Played key role in recognition of The Carnegie Mellon University GBMES chapter at national BMES meeting.

PENN BIOTECH GROUP

Philadelphia, PA 1/07-4/07

Student Consultant

Offered targeted recommendations on technological improvements with a focus on increasing product marketability for a medical device startup company. Researched and presented on competing technologies and collaboration opportunities.

NASA JET PROPULSION LABORATORY

Pasadena, CA 8/05-10/05

Space Grant Summer Research Intern

Analyzed ratios of isomers of amino acids in soil samples collected from Atacame Desert, Chile for signs of life as an analog to Martian surface. Garnered in-depth knowledge and effectively collaborated with NASA staff. Noted for successfully utilizing HPLC and purification methods to garner insights.

LOUISIANA STATE UNIVERSITY

Baton Rouge, LA 6/04-8/04

Agronomy Biotechnology Intern

Received hands-on instruction on biotechnology techniques such as gene gun usage, PCR process, and sample DNA extraction. Facilitated sugarcane genetics (microsatellite) research project and maintained detailed documentation.

Education & Advanced Training

Master of Science in Biomedical Engineering (12/10)

Thesis: Synergistic Effects of BMP-2 and PDGF-BB on Proliferation and Differentiation of Human Mesenchymal Stem Cells

Carnegie Mellon University, PA

Teaching Assistantships at Carnegie Mellon University:

• Graded exams, addressed particular student needs, and held weekly office hours for Physiology (42-202) taught by Professor Phil Campbell (Spring 2010).

• Taught laboratory classes and produced laboratory manual with background info and SOPs for Biomaterial/Host Interactions (42-419) taught by Professor Jeffrey O. Hollinger (Fall 2009).

• Offered 1-on-1 student support for Physiology (42-202) taught by Professor Kris Dahl (Spring 2009).

Master of Biotechnology (Biopharmaceutical/Engineering, Biotechnology Track) (8/07)- University of Pennsylvania, PA

Bachelor of Science in Biological Engineering (5/06)- Louisiana State University, LA

Bachelor of Science in Biological Sciences (5/05)- Louisiana State University, LA

Publications & Presentations

• Aditi Sharma, Jinku Kim, Brett Runge, Pedro Alvarez, Mahrokh Dadsetan, Michael J. Yaszemski, Jeffrey O. Hollinger. Potential of Poly(ε-caprolactone fumarate) as a Bone Tissue Engineering Scaffold. Poster presentation at the Society for Biomaterials Annual Meeting, Seattle, WA, USA.

• Mark Wong DDS, Ayesha Nawab DMD, MD, Dan Kwak, Aditi Sharma, Lyndsey Schutte, Bruce A. Doll, DDS, PhD. Chapter 3: Osseous Wound Healing; An Introduction to Biomaterials. 2nd Edition. Jeffrey O. Hollinger, DDS, PhD.

• Jinku Kim, Aditi Sharma, Runge Brett, Bruce Doll, Heather Waters, Pedro Alvarez, Dadsetan Mahrokh, Michael J. Yaszemski, and Jeffrey O. Hollinger. Osteoblast growth and bone-healing response to three-dimensional poly(ε-caprolactone fumarate) scaffolds. Journal of Tissue Engineering and Regenerative Medicine. 2011.

• Aditi Sharma, Jinku Kim, Brett Runge, Pedro Alvarez, Mahrokh Dadsetan, Michael J. Yaszemski, Jeffrey O. Hollinger. Potential of Poly(ε-caprolactone fumarate) as a Bone Tissue Engineering Scaffold. Poster presentation at the AFIRM All-Hands Meeting, Tampa, FL, USA. January 2010.

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