Medical Assistant

Theodros Tadese

***** ********** *****

Silver Spring, Maryland 20904

Tel. 517-***-**** (Mobile) Email: ********@*****.***

----------------------------------------------------------------------------------------------

OBJECTIVE

Seek progressively challenging opportunity which might involve:

• Managing programs or directing clinical, technical laboratory services and research facilities involving virological/microbiological, molecular, immunological, diagnosis and characterization.

• Undertaking research projects involving vaccine development, safety and efficacy evaluation, or molecular mechanisms of pathogenesis of viral or bacterial infections of public health concern and/or zoonotic.

Education: Michigan State University, East Lansing, MI.

PhD Microbiology/Virology and Animal sciences- 1993

Master of Science in Microbiology and Public Health 1986

Haile Selassie I University, Addis Ababa, Ethiopia.

Bachelors of Science in Biology and Chemistry 1979

Work Experience Center for Microbiology Excellence, Neogen Corporation, East Lansing, MI Research

Research –Team Leader Microbiology-Pathogen Detection, R & D. 2008 -2011

•Planned, designed and implemented microbiological food safety research projects: developed and validated testing methods and protocols for detection of food-borne pathogens (eg. Salmonella, E. coli, Listeria and Campylobacter spp.) from diverse food commodities (beef, poultry, vegetable, pets, feed) and from environmental samples.

•Optimized media formulations for immuno-diagnostic techniques (eg. lateral flow chromatography) and DNA based formats (eg. Genequence) for the identification and differentiation of food-borne pathogens; developed and evaluated neutraceuticals and biologics, efficacy studies of disinfectants and anti-microbial agents.

• Lead projects; coordinated objectives and control operation of the microbiology laboratory, review quality system policies and procedures, quality system manual (QSM) and ISO manuals, developed standard operating procedure (SOP), analytical procedures and preventive maintenance (PM), evaluated and interpreted experimental data; prepared manuscripts, summary, protocols and technical bulletins; presented data at seminars and conferences.

Pathobiology and Diagnostic Investigation/ DCPAH, MSU, East Lansing, MI

Senior Research Scientist 1997- 2008

• Managed and supervised a diagnostic and research laboratory involving molecular, immunological, biochemical and serological techniques for viruses and bacteria; oversee compliance with GLPs and with federal and office of radiation, chemical and biological safety (ORCBS) policies and regulations.

• Devised, planned and undertook independent research involving molecular mechanisms of pathogenesis of vaccine viruses: identified and characterized virulence factors, contaminant or integrated foreign genetic material, specific genetic and antigenic markers of newly emerged variants, conducted cross protection studies, evaluated efficacy and safety of viral vaccines, selected novel candidate vaccine strains.

• Undertook specific research projects: pathogenesis and molecular characterization of viruses, including reticuloendotheliosis virus (REV), infectious laryngotracheitis virus (ILTV), avian poxviruses (APV, and salmonella sp.

• Performed a wide range of immunoassay and molecular biology techniques (Real-Time PCR, multiplex PCR, Southern and Western blots, Immunofluorescence (IF), Immunohistochemistry (IHC), restriction fragment length polymorphism (RFLP), fluorescent in situ hybridization (FISH), 2-D electrophoresis, ELISA, agar gel precipitation test (AGPT), agar gel enzyme assay (AGEA) etc.

• Developed and validated diagnostic assays and molecular techniques for specific experiments and applications; prepared primary tissue cultures and established cell lines.

• Trained and evaluated laboratory personnel; research assistants, lab aides and students.

• Documented findings, published results in peer-reviewed journals, presented research findings.

Microbiology Section

Research Associate 1993- 1996

• Undertook research involving molecular characterization of anaerobic bacteria and their bacteriophages from the gastrointestinal tract and rumen.

• Conducted in vitro fermentation experiments using anaerobic Hungate technique roll tube culture.

• Performed Chromatographic, electron microscopic, pulsed field gel electrophoresis (PFGE), southern hybridization, molecular cloning, cDNA, nucleic acid probes.

Graduate Student (MS, PhD) 1985-1993

• Project involved identification and isolation of a novel bacteriophage, isolation of cellulose, hemicellulose and pectin degrading bacterial strains by Hungate anaerobic techniques.

• Confirmed new bacteriophage by transmission electron microscopy and agar overlay techniques.

• Designed, conducted in vitro fermentation assays, analyzed by-products by gas chromatographic techniques, analyzed data using statistical package, SAS.

• Served as a teaching assistant for several courses in microbiology, toxicology, nutrition and molecular biology.

• Undertook research project involving analysis of polypeptide-specific immune response against porcine herpes virus (PHV) by radio-immunoprecipitation.

• Maintained cell culture of crandeel rees feline kidney cell lines; tested balb c mice for antibodies by enzyme linked immunosorbent assay (ELISA); purified virus by density gradient centrifugation.

• Performed Southern, Northern blots, agarose and gel electrophoresis.

National Institute of Health and Research, Addis Ababa, Ethiopia

Research Assistant Virology section 1979-1984

• Organized, conducted sero-epidemiological and prevalence studies and diagnosis of measles, rotavirus, hepatitis A and B viruses, rubella virus, polio virus, influenza virus, published results.

• Performed bacteriological, serological, parasitological, clinical chemistry and virological examinations of a variety of biological samples, specimens, products and food stuffs.

• Trained senior technicians and laboratory personnel in public health and virological related courses and diagnostic techniques.

• Established and maintained cell culture of African green monkey kidney (AGMK) cells.

• Performed techniques for rapid diagnosis of viral infection using hemolysis in gel (HIG), hemagglutination inhibition (HI), complement fixation (CF), immunoelectrophoresis (IEOP), enzyme linked immunosorbent assay (ELISA), neutralization test (NT) immunofluorescent techniques (IF) and LD50 test.

Computer Skills: Operating Systems: Windows, UNIX; Software: SAS, bioinformatics (Seqweb, GCG Sequencer, NCBI) Microsoft Word, Photo Adobe, Power Point, Excel.

Additional Skills: Peer-Reviewer for Journal of General Virology, Medical Science Monitor, Expert Review of Vaccines, Vaccine.

Affliations: Journal of General Virology, Journal of Medical Microbiology, American Society for Microbiology, International Journal of Systematic and Evolutionary Microbiology, American Veterinary Medical Association, American Association for Avian Pathologists.

Certifications: Building Leadership in the Workplace Certificate, June 2009

American Veterinary Medical Association certificate, July 2007

Certificate of Appreciation, Department of Pathology, 2002

Care and Use of Laboratory Animals, University Laboratory Animal Resources, Oct 1998

Professional Publications:

Rebecca Horner, Jill Feldpausch, R. Lucas Gray, Stephanie Curry, Zahid Islam, Tim Goldy, Frank Klein, Theodros Tadese, Jennifer Rice, Mark Mazola. 2011. Reveal Salmonella 2.0 test for detection of Salmonella spp. in food and environmental samples. J. of AOAC International 94: No.5. 1467-1480

Tadese, T., S. Fitzgerald and W.M. Reed. 2008. Detection and differentiation of re-emerging of fowlpox virus (FPV) strains carrying integrated reticuloendotheliosis virus (FPV-REV) by Real-Time PCR. Vet. Microbiol. 127: 39-49

Tadese, T., E. Potter, S. Fitzgerald, and W. M. Reed. 2007. Concurrent infection in chickens with fowlpox virus (FPV) and infectious laryngotracheitis virus (ILT) as detected by immunohistopathology and multiplex PCR techniques. Avian Diseases 51: 719-724.

Tadese, T., and W. M. Reed. 2003. Detection of specific reticuloendotheliosis virus sequence and protein from REV-integrated fowlpox virus strains. J. Virol. Methods 110 (1): 99-104.

Tadese, T., E. Potter, and W. M. Reed. 2003. Development of a mixed antigen agar gel enzyme assay (AGEA) for the detection of antibodies to poxvirus in chicken and turkey sera. J. Vet. Med. Sci. 65(2): 255-258

Tadese, T., and W.M. Reed. 2003. Use of restriction fragment length polymorphism (RFLP), immunoblotting (IB) and polymerase chain reaction (PCR) in the differentiation of avian poxviruses. J. Vet. Diagn. Invest 15: 141-150

Tadese, T., S. Fitzgerald, and W. M. Reed. 2012. Genetic and antigenic evidence of reticuloendotheliosis virus (REV) integrated avian poxvirus (APV-REV) infection of wild and exotic birds. To be resubmitted.

Tadese, T., and M.T. Yokoyama. 1994. Isolation and characterization of a temperate bacteriophage from Ruminococcus albus, a predominant ruminal cellulolytic anaerobe.

Tadese, T. 1985. Survey of rotavirus infection in acute gastroenteritis in Addis Ababa. A comparison between ELISA and IEOP. E. Afr. Med. J. 62: 8. 580

Tadese, T., and B.Ghiorgis. 1985. Measles immunity in children before one year of age. Ethiop. Med. J 23:17

Abstracts and Presentations:

Tadese T. 2008. Conventional versus molecular diagnostic approaches to Salmonella species, Department of Health, Public health laboratory, 300 Indian Ave, NW Washington D.C. June 12.

Tadese, T and W.M. Reed. 2006. Genetic and serologic evidence of reticuloendotheliosis virus (REV) integrated avian poxvirus (APV-REV) infection of wild and exotic birds. 143 rd American Veterinary Medical Association (AVMA) annual convention, Honolulu, Hawaii, July 15-19.

Tadese, T., E. Potter, and W.M. Reed. 2004. Real-Time PCR for the detection of Fowlpox virus (FPV) strains carrying integrated reticuloendotheliosis virus (FPV-REV). 141st American Veterinary Medical Association (AVMA) annual convention, Philadelphia, July 24-28.

Tadese, T., and W.M. Reed. 2002. Localization and profiling of reticuloendotheliosis virus (REV) protein/s in cultures infected with REV integrated fowlpox virus (FPV-REV) strains. 139th American Veterinary Medical Association (AVMA) annual convention, Nashville, July 13-17.

Tadese, T., W.M. Reed and A.M. Fadly. 2000. Detection of specific reticuloendotheliosis virus (REV) nucleotide sequences and proteins in putatively REV-integrated fowl poxvirus (FPV) strains. 137th American Veterinary Medical Association (AVMA) annual convention, Salt Lake City, Utah, July 22-26.

Tadese, T. and W.M. Reed. 1999. Polymerase Chain Reaction for the Diagnosis of Avian Poxvirus infection. 136th American Veterinary Medical Association (AVMA) annual convention, New Orleans, July 10-14.

Tadese, T., E. Potter., and W.M. Reed. 1997. Genetic and antigenic differences between vaccine strains and field isolates of fowl poxvirus. 78th annual meeting of the conference of research workers in Animal Diseases (CRWAD) proceedings, Chicago, Nov. 10-11. No. 237.

Tadese, T., and M.T.Yokoyama. 1989. Bacteriophage associated with a rumen cellulolytic bacteria Ruminococcus albus. XX Biennial conference on Rumen Function, Chicago, IL, Nov. 7-9.

Tadese, T., K.L.Klomparens., M.E.Benson., S.R.Rust., and M.T.Yokoyama. 1989. Occurrence of rumen bacteriophages with Treponema saccharophilum and Butyrivibrio fibrisolvens. Rpt of Beef Cattle -Forage Research. Farm Science, MI Agri. Experiment Station.

Yokoyama, M.T., and T. Tadese. 1987. Attachment of bacteriophages to rumen bacteria. XIX. Biennial conference on Rumen Function, Chicago, IL, Nov. 119.

Melamed, M.D., T. Tadese., E. Habtegaber., H. Kefene. 1981. Measurement of hepatitis and markers and alfa-feto protein in Ethiopia. Ethiop. Med. J. 19 (4).

Melamed, M.D., T. Tadese., and G. Adal. 1981. Measles immunity and vaccination response in Addis Ababa. Ethiop. Med. J. 19 (3).

Contact this candidate